[1]徐丽,陈新,宗晓娟,等.樱桃砧木PcWRKY1基因的克隆与表达分析[J].江苏农业学报,2018,(03):636-641.[doi:doi:10.3969/j.issn.1000-4440.2018.03.023]
 XU Li,CHEN Xin,ZONG Xiao-juan,et al.Cloning and expression analysis of PcWRKY1 gene in cherry rootstocks[J].,2018,(03):636-641.[doi:doi:10.3969/j.issn.1000-4440.2018.03.023]
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樱桃砧木PcWRKY1基因的克隆与表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年03期
页码:
636-641
栏目:
园艺
出版日期:
2018-06-25

文章信息/Info

Title:
Cloning and expression analysis of PcWRKY1 gene in cherry rootstocks
作者:
徐丽陈新宗晓娟朱东姿魏海蓉王甲威谭钺张力思刘庆忠
(山东省果树研究所/山东省果树生物技术重点实验室,山东泰安271000)
Author(s):
XU LiCHEN XinZONG Xiao-juanZHU Dong-ziWEI Hai-rongWANG Jia-wei TAN YueZHANG Li-siLIU Qing-zhong
(Shandong Province Key Laboratory of Fruit Tree Biotechnology /Shandong Institute of Pomology,Tai’an 271000,China)
关键词:
樱桃砧木WRKY转录因子基因表达
Keywords:
cherry rootstockWRKY transcription factorgene expression
分类号:
Q786
DOI:
doi:10.3969/j.issn.1000-4440.2018.03.023
文献标志码:
A
摘要:
WRKY转录调控因子广泛参与植物的生长发育、生物胁迫和非生物胁迫等生理过程。为明确干旱和盐等非生物胁迫对樱桃砧木PcWRKY1基因表达的影响,本研究利用RT-PCR技术克隆了樱桃砧木Gisela 6(Prunus cerasus × Prunus canescens)的WRKY基因cDNA序列,命名为PcWRKY1(GenBank登录号为KY399985)。序列分析结果表明,PcWRKY1完整开放阅读框为 1 461 bp,编码486个氨基酸,编码的蛋白质含有2个WRKY保守结构域和2个C2H2锌指结构域,属于WRKY家族第Ⅰ类成员。qRT-PCR分析PcWRKY1在200 mmol甘露醇和200 mmol NaCl胁迫下的表达情况,结果显示,甘露醇和NaCl均能诱导PcWRKY1基因表达,推测PcWRKY1基因在应对干旱和盐2种非生物胁迫方面可能具有重要作用。
Abstract:
The WRKY transcription factors are widely involved in the physiological processes related to plant growth and development, biotic and abiotic stresses. A full-length cDNA for PcWRKY1 (GenBank accession number KY399985) gene was cloned from the cherry (Prunus) rootstock Gisela 6(Prunus cerasus × Prunus canescens) using reverse transcription-polymerase chain reaction (RT-PCR). Sequence analysis results showed that the PcWRKY1 gene was found to contain an open reading frame (ORF) with 1 461 bp and encoded 486 amino acids. The protein encoded by PcWRKY1 comprised of two conserved WRKY domains and two zinc finger structures (C2H2), and thus, belonged to Group I of the WRKY family. Furthermore, the expression of PcWRKY1 under the stresses of 200 mmol mannitol and 200 mmol NaCl was analysed by quantitative RT-PCR. Both mannitol and NaCl could induce the expression of PcWRKY1 gene, suggesting that PcWRKY1 played an important role in dealing with drought and salt stress.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-09-16 基金项目:山东省农业科学院青年科研基金项目(2016YQN25);公益性行业(农业)科研专项(201203075);山东省水果产业体系创新团队建设项目(SDAIT-03-022-04) 作者简介:徐丽(1983-),女,山东曲阜人,博士,助理研究员,主要从事果树资源与分子技术研究。(E-mail)xuli1245@163.com 通讯作者:刘庆忠,(E-mail)qzliu001@126.com
更新日期/Last Update: 2018-07-04