[1]冯娇,侯旭东,董礼花,等.GA3与CPPU对葡萄果锈相关物质合成及基因表达的影响[J].江苏农业学报,2018,(03):642-650.[doi:doi:10.3969/j.issn.1000-4440.2018.03.024]
　FENG Jiao,HOU Xu-dong,DONG Li-hua,et al.Effects of GA3 and CPPU on the synthesis of related substances and gene expression of berry russet of grapes[J].,2018,(03):642-650.[doi:doi:10.3969/j.issn.1000-4440.2018.03.024]
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GA3与CPPU对葡萄果锈相关物质合成及基因表达的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年03期
页码:
642-650
栏目:
园艺
出版日期:
2018-06-25

文章信息/Info

Title:
Effects of GA3 and CPPU on the synthesis of related substances and gene expression of berry russet of grapes
作者:
冯娇1; 侯旭东1; 董礼花2; 陶建敏1
（1.南京农业大学园艺学院，江苏南京210095；2.灌南县蔬菜办公室，江苏灌南223500）
Author(s):
FENG Jiao1; HOU Xu-dong1; DONG Li-hua2; TAO Jian-min1
(1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Vegetable Office of Guannan County, Guannan 223500, China)
关键词:
阳光玫瑰; 果锈; GA3+CPPU处理; 基因表达; 次生代谢物
Keywords:
Shine Muscat; fruit russet; the treatment of GA3 and CPPU; gene expression;  secondary metabolites
分类号:
S663.1
DOI:
doi:10.3969/j.issn.1000-4440.2018.03.024
文献标志码:
A
摘要:
为探究葡萄的防锈措施，本研究以阳光玫瑰葡萄为试验材料，于花后14 d，用25 mg/L的GA3分别和0 mg/L、5 mg/L、10 mg/L、15 mg/L的CPPU组合处理果穗，以清水处理为对照（CK）。待果实进入转色期后分别采集不同成熟阶段的葡萄果实，通过实时荧光定量PCR测定与果锈生成相关的苯丙氨酸解氨酶（PAL）基因和白藜芦醇合酶（RS）基因的表达水平，测定葡萄果皮果锈发生率、果皮中次生代谢物含量等指标。结果表明，果实成熟过程中，与对照相比，GA3+CPPU处理后，PAL和RS基因表达水平降低，PAL基因表达峰值、RS基因表达水平均与CPPU处理浓度呈负相关。与对照相比，GA3+CPPU处理显著降低了果皮中白藜芦醇、总酚和木质素含量，咖啡酸、香豆酸、丁香酸等酚酸含量随CPPU浓度增加显著下降，儿茶素和没食子酸含量随CPPU处理浓度增大先升后降。GA3+CPPU处理的葡萄果皮果锈发生率明显降低。与GA3+5 mg/L CPPU处理的葡萄果实相比，GA3+10 mg/L CPPU和GA3+15 mg/L CPPU处理的葡萄果实成熟期推迟，风味下降，商品性显著降低。因此，生产实践上用GA3+CPPU处理阳光玫瑰葡萄果穗的适宜质量浓度为25 mg/L GA3＋5 mg/L CPPU。
Abstract:
In order to explore the russet prevention measures of grapes, table grape cultivar Shine Muscat was used as material. On the 14th day after anthesis, grape fruit clusters were treated with 25 mg/L GA3（Gibberellin A3）+0 mg/L CPPU（forchlorfenuron）， 25 mg/L GA3+5 mg/L CPPU， 25 mg/L GA3+10 mg/L CPPU and 25 mg/L GA3+15 mg/L CPPU， respectively, and treated with water as control(CK). After the fruit entered veraison, grape fruits in different stages of maturity were collected separately. The expression levels of phenylalanine ammonia lyase (PAL) gene related to the formation of fruit russet and resveratrol synthase (RS) gene were measured by real-time fluorescence quantitative PCR. The fruit skin russet level and secondary metabolites content in grape peel were determined. The results show that, compared with the control, the gene expression level of PAL and RS after CPPU treatment were decreased during the process of fruit ripening. The expression peak value of PAL gene and the expression level of RS gene negatively correlated with the concentration of CPPU treatment. To compared with those in control, the contents of total phenol, resveratrol and lignin in pericarp of berries were significantly decreased in the treatment of GA3 and CPPU. With the increase of CPPU concentration, the content of phenolic acids such as caffeic acid, fenugreek acid and clove acid decreased significantly however, the content of catechin and gallic acid increased first and then decreased. The incidence of berry russet was reduced significantly under the treatment of GA3 and CPPU. Compared with that of the grape berries treated with GA3 and 5 mg/L CPPU, the maturity of the grape berries treated with GA3 and 10 mg/L CPPU or 15 mg/L CPPU was delayed, the flavor was decreased and the marketability was decreased significantly. Therefore, the optimum treatment was 25 mg/L GA3 combined with 5 mg/L CPPU.

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相似文献/References:

[1]冯娇,王武,侯旭东,等.基于转录组测序技术探究GA3和CPPU抑制葡萄果锈产生的机理[J].江苏农业学报,2017,(04):895.[doi:doi:10.3969/j.issn.1000-4440.2017.04.026]
　FENG Jiao,WANG Wu,HOU Xu-dong,et al.Underlying mechanism of GA3 and CPPU inhibiting grape fruit russet discovered based on transcriptome sequencing[J].,2017,(03):895.[doi:doi:10.3969/j.issn.1000-4440.2017.04.026]

备注/Memo

备注/Memo:
收稿日期：2017-09-26 基金项目：国家现代农业葡萄产业技术体系项目（CARS-29）；国家“948”重点项目（2016-X19）；江苏省农业三新工程项目[SXGC(2017)274、SXGC(2016)001]; 江苏省农业重大新品种创制项目（PZCZ201723） 作者简介：冯娇（1992-），女，山西运城人，硕士,助理农艺师，主要从事果树生理与分子技术研究。（E-mail）478710492@qq.com 通讯作者：陶建敏，（E-mail）taojianmin@njau.edu.cn
更新日期/Last Update: 2018-07-04