[1]徐小波,章熙霞,王公金,等.猪极体的排出与退化规律及其活性保存[J].江苏农业学报,2018,(03):599-604.[doi:doi:10.3969/j.issn.1000-4440.2018.03.017]
　XU Xiao-bo,ZHANG Xi-xia,WANG Gong-jin,et al.The excretion and degeneration regularity of porcine polar bodies and its activity preservation[J].,2018,(03):599-604.[doi:doi:10.3969/j.issn.1000-4440.2018.03.017]
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猪极体的排出与退化规律及其活性保存()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年03期
页码:
599-604
栏目:
畜牧兽医·水产养殖
出版日期:
2018-06-25

文章信息/Info

Title:
The excretion and degeneration regularity of porcine polar bodies and its activity preservation
作者:
徐小波1; 章熙霞2; 王公金1; 谭小东1; 周晓龙1
(1.江苏省农业科学院畜牧研究所/农业部种养结合重点实验室，江苏南京210014；2.南京市畜牧家禽研究所，江苏南京210036)
Author(s):
XU Xiao-bo1; ZHANG Xi-xia2; WANG Gong-jin1; TAN Xiao-dong1; ZHOU Xiao-long1
(1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Crop and Livestock Integrated Farming, Ministry of Agriculture, Nanjing 210014, China;2.Institute of Animal and Poultry Science of Nanjing, Nanjing 210036, China)
关键词:
猪卵母细胞; 第一极体; 第二极体; 存活率
Keywords:
porcine oocyte; first polar body; second polar body; survival rate
分类号:
S828.1
DOI:
doi:10.3969/j.issn.1000-4440.2018.03.017
文献标志码:
A
摘要:
哺乳动物卵母细胞二次减数分裂分别产生第一极体（PbⅠ）和第二极体（PbⅡ），极体（Pb）含有与卵母细胞相似的遗传物质。为探索猪Pb的排出与退化规律，并建立有效的Pb保存方法，从屠宰后的母猪卵巢中采集卵母细胞进行体外成熟培养及体外受精，分别对PbⅠ和PbⅡ的排出与退化规律及其在不同保存温度下的存活情况进行研究。结果表明：PbⅠ在卵泡卵母细胞体外培养40 h时排出率最高，PbⅠ在39 ℃条件下保存6 h形态正常率为85.5%，但存活率只有18.2%，4 ℃保存40 h存活率为 85.0%，-20 ℃保存168 h存活率为95.0%，-196 ℃超低温保存1 344 h存活率和形态正常率分别达89.1%和97.8%。PbⅡ在卵母细胞受精后20 h排出率最高，PbⅡ在39 ℃条件下保存16 h形态正常率为69.0%，但存活率只有34.5%，4 ℃保存30 h存活率为87.5%，-20 ℃保存240 h存活率为84.6%，-196 ℃超低温保存1 344 h存活率和形态正常率分别达87.0%和95.7%。
Abstract:
The mammalian oocytes produce the first polar body（PbⅠ）and the second polar body (PbⅡ) during the two meiosis, respectively. The polar bodies (Pbs) contain some genetic materials, which are similar to the oocytes. To explore the regularity of excretion and degeneration of pig Pb and establish effective method of Pb preservation, the oocytes were collected from the porcine ovaries for in vitro maturation and in vitro fertilization. The excretion and degeneration of PbⅠ and PbⅡ and their survival condition at different storage temperatures were investigated. The results showed that the excretion rate of PbⅠ was highest at 40 h in vitro culture, the survival rate and morphologically normal rate of PbⅠ were 18.2% and 85.5% when cultured at 39 ℃ for 6 h, the survival rate of PbⅠ was 85.0% when conserved at 4 ℃ for 40 h, the survival rate of PbⅠ was 95.0% when conserved at -20 ℃ for 168 h, the survival rate and morphologically normal rate were 89.1% and 97.8% when cryopreserved at -196 ℃ for 1 344 h, respectively. PbⅡ had the highest excretion rate at 20 h after oocyte fertilization, the survival rate and morphologically normal rate of PbⅡ were 34.5% and 69.0%, respectively, when cultured at 39 ℃ for 16 h, the survival rate of PbⅡ was 87.5% when conserved at 4 ℃ for 30 h, the survival rate of PbⅡ was 84.6% when conserved at -20 ℃ for 240 h, the survival rate and morphologically normal rate were 87.0% and 95.7%, respectively, when cryopreserved at -196 ℃ for 1 344 h.

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备注/Memo

备注/Memo:
收稿日期：2017-09-14 基金项目：农业部转基因生物新品种培育基金项目(2014ZX08006-004) 作者简介：徐小波(1964-)，男，江苏镇江人，硕士，研究员，主要从事猪种质资源保存利用与开发研究。(E-mail) 119649103@qq.com 通讯作者：章熙霞，(E-mail) zxx6618@sohu.com
更新日期/Last Update: 2018-07-04