[1]苏赵,胡凯弟,朱佳雯,等.地衣芽孢杆菌B-1降解西维因的环境条件[J].江苏农业学报,2018,(03):585-590.[doi:doi:10.3969/j.issn.1000-4440.2018.03.015]
　SU Zhao,HU Kai-di,ZHU Jia-wen,et al.Environmental conditions of carbaryl degradation by Bacillus licheniformis B-1[J].,2018,(03):585-590.[doi:doi:10.3969/j.issn.1000-4440.2018.03.015]
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地衣芽孢杆菌B-1降解西维因的环境条件()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年03期
页码:
585-590
栏目:
耕作栽培·资源环境
出版日期:
2018-06-25

文章信息/Info

Title:
Environmental conditions of carbaryl degradation by Bacillus licheniformis B-1
作者:
苏赵1; 胡凯弟1; 朱佳雯1; 王兴洁1; 刘书亮1; 2
(1.四川农业大学食品学院，四川雅安625014；2.四川农业大学食品加工与安全研究所，四川雅安625014)
Author(s):
SU Zhao1; HU Kai-di1; ZHU Jia-wen1; WANG Xing-jie1; LIU Shu-liang1; 2
(1.College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;2.Research Institute of Food Processing and Security, Sichuan Agricultural University, Ya’an 625014, China)
关键词:
地衣芽孢杆菌; 西维因; 降解; 环境修复
Keywords:
Bacillus licheniformis; carbaryl; degradation; environmental remediation
分类号:
X592
DOI:
doi:10.3969/j.issn.1000-4440.2018.03.015
文献标志码:
A
摘要:
以地衣芽孢杆菌B-1(Bacillus licheniformisB-1) 为材料，采用HPLC测定不同微生物培养体系中西维因(Carbaryl)残留量，并考察菌株B-1降解谱，探究其对西维因的降解特性。结果表明，菌株B-1对西维因的降解率与其生长呈正相关，在LB培养基中培养72 h对100 mg/L西维因的降解率为91.21%。较高的温度和碱性环境有利于西维因的降解，而较高底物质量浓度和盐质量浓度均对菌株B-1的生长及西维因的降解有负面影响；Ca2+、Fe2+、Mn2+、Mg2+对其降解西维因有不同程度的刺激作用，Cu2+则有抑制作用。此外，菌株B-1对20 mg/L的丁硫克百威、叶蝉散和毒死蜱均有不同程度的降解作用。HPLC分析结果表明，初步确定1-萘酚是菌株B-1降解西维因的中间产物，且该菌株在LB培养基中培养72 h对20 mg/L 1-萘酚的降解率为48.70%。可见，菌株B-1能高效降解西维因和其他多类杀虫剂，具有一定的广谱性，可应用于环境修复。
Abstract:
Degradation characteristics of carbaryl by strain Bacillus licheniformis B-1 were studied. Concentrations of carbaryl residue in different microbial culture systems were determined through HPLC, and degradation spectrum of strain B-1 was explored. The results showed that degradation of carbaryl had a positive correlation with the growth of strain B-1. Degradation rate reached up to 91.21% when the strain was cultured in LB medium with 100 mg/L carbaryl for 72 h. Higher temperatures and alkaline circumstance were advantageous for degradation of carbaryl. Simultaneously, growth of strain B-1 and carbaryl degradation were inhibited with increasing substrate concentrations or salinity. Carbaryl degradation were stimulated when treating with metal ions such as Ca2+, Fe2+, Mn2+, Mg2+, whereas inhibited by Cu2+. In addition, this strain had degradation effect on 20 mg/L carbosulfan, isoprocarb and chlorpyrifos with different rate. HPLC results showed that 1-naphthol was an intermediate product of carbaryl, and strain B-1 could degrade 48.70% of 1-naphthol (20 mg/L) in LB medium after 72 h incubation. In conclusion, strain B-1 can efficiently degrade carbaryl and other kinds of insecticides, which shows that strain B-1 possesses a wide-spectrum degrading activity and potential for environmental remediation.

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备注/Memo

备注/Memo:
收稿日期：2017-08-13 基金项目：四川省农业科技成果转化基金项目(14NZ0012) 作者简介：苏赵(1959-)，男，四川雅安人，大学，实验师，主要从事食品加工与安全方面的教学和科研工作。(Tel)0835-2882187 通讯作者：刘书亮，(E-mail)lsliang999@163.com
更新日期/Last Update: 2018-07-04