[1]李雅洁,张其安,陆晓民.不同外源物质对低温弱光次生盐渍化复合逆境下黄瓜幼苗生长、抗氧化系统及光合作用的影响[J].江苏农业学报,2018,(02):404-410.[doi:doi:10.3969/j.issn.1000-4440.2018.02.026]
 LI Ya-jie,ZHANG Qi-an,LU Xiao-min.Effect of different exogenous substances on the growth, antioxidant system and photosynthesis of cucumber seedling under the adverse temperature, light, salt complex condition[J].,2018,(02):404-410.[doi:doi:10.3969/j.issn.1000-4440.2018.02.026]
点击复制

不同外源物质对低温弱光次生盐渍化复合逆境下黄瓜幼苗生长、抗氧化系统及光合作用的影响()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
404-410
栏目:
园艺
出版日期:
2018-04-25

文章信息/Info

Title:
Effect of different exogenous substances on the growth, antioxidant system and photosynthesis of cucumber seedling under the adverse temperature, light, salt complex condition
作者:
李雅洁12张其安3陆晓民1
(1.安徽科技学院,安徽凤阳233100;2.怀远县农业委员会,安徽怀远233400;3.安徽省农业科学院园艺研究所,安徽合肥230031)
Author(s):
LI Ya-jie12ZHANG Qi-an3LU Xiao-min1
(1.Anhui Science and Technology University, Fengyang 233100, China;2.Agricultural Committee of Huaiyuan County, Huaiyuan 233400, China;3.Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, China)
关键词:
黄瓜低温弱光次生盐渍化复合逆境外源物质幼苗生长抗氧化系统光合作用
Keywords:
cucumberadverse temperature light salt complex conditionexogenous substanceseedling growthantioxidant systemphotosynthesis
分类号:
S642.2
DOI:
doi:10.3969/j.issn.1000-4440.2018.02.026
文献标志码:
A
摘要:
以黄瓜品种津优35号为试验材料,研究在低温弱光次生盐渍化
[16 ℃/10 ℃、(140±10)μmol/( m2· s)、50 mmol/L Ca( NO3) 2]复合逆境胁迫下分别喷施精胺、油菜素内酯、三十烷醇、褪黑素、腐胺对黄瓜幼苗生长、抗氧化系统和光合作用的影响。结果表明,低温弱光次生盐渍化复合逆境下黄瓜幼苗的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性降低,而过氧化物酶(POD)活性升高,丙二醛(MDA)累积量增加,膜透性增强,幼苗的光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)及胞间CO2浓度(Ci)显著下降,幼苗的干质量、鲜质量分别显著减少,生长受到显著抑制。叶面喷施油菜素内酯、三十烷醇、褪黑素、腐胺可有效提高低温弱光次生盐渍化复合逆境下黄瓜幼苗的抗氧化酶活性,减轻其膜脂过氧化程度,增强其光合性能,显著增加幼苗干质量、鲜质量,综合比较以三十烷醇和腐胺的喷施效果最佳,其次是油菜素内酯和褪黑素处理。
Abstract:
An experiment was conducted to investigate the effects of spermine (Spm), Epi-brassinolide (EBR), triacontanol (TA), melatonin(MT) and putrescine (Put) on growth, antioxidant system and photosynthesis of cucumber seedlings under complex stress
[16 ℃/10 ℃,140±10μmol/( m2· s),50 mmol/L Ca(NO3)2]. Compared with the control, the activity of superoxide dismutase and catalase decreased, the peroxidase activity, the malondialdehyde accumulation and cell membrane permeability significantly increased, the leaf net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), intercellular CO2 concentration (Ci), dry and fresh matter accumulation decreased in the adverse environment, respectively. However, applying EBR, TA, MT and Put could further improve the antioxidant enzyme activity,reduce the membrane permeability of seedlings,make the leaves keep a higher photosynthetic rate, promote the seedlings growth. Comprehensive comparison, TA and Put treatment were most effective, followed by the EBR and MT treatment.

参考文献/References:

[1]严斌,丁小明,魏晓明. 我国设施园艺发展模式研究[J]. 中国农业资源与区划, 2016, 37(1): 196-201.
[2]蒋卫杰,邓杰,余宏军. 设施园艺发展概况、存在问题与产业发展建议[J]. 中国农业科学, 2015,48(17): 3515-3523.
[3]魏晓明,齐飞,丁小明,等. 我国设施园艺取得的主要成就[J]. 农机化研究, 2010, 32(12): 227-231.
[4]郭世荣,孙锦,束胜,等. 我国设施园艺概况及发展趋势[J]. 中国蔬菜, 2012(18): 1-14.
[5]李怀智. 我国黄瓜栽培的现状及其发展趋势[J]. 蔬菜, 2003(8): 3-4.
[6]安志信,孟庆良,刘文明. 黄瓜的起源和传播初析[J]. 长江蔬菜, 2006(1): 39-40.
[7]李光,付海鹏,杜胜利. 我国黄瓜新品种应用和良种生产现状[J].长江蔬菜, 2007(1): 30-32.
[8]崔建云,董晨娥,左迎之,等. 外部环境气象条件对日光温室气象条件的影响[J]. 气象, 2006, 32(3): 101-106.
[9]曹齐卫,李利斌,孔素萍,等. 我国设施黄瓜新育成品种芽期耐盐性评价[J]. 植物遗传资源学报, 2014, 15(3): 546-553.
[10]陆晓民,高青海. 油菜素内酯对硝酸钙胁迫下黄瓜幼苗生长及其抗氧化同工酶表达的影响[J]. 热带作物学报, 2011, 32(11): 2104-2108.
[11]AN Y, ZHOU H, MIN Z, et al. Root proteomics reveals cucumber 24-epibrassinolide responses under Ca(NO3) 2 stress[J]. Plant Cell Reports, 2016, 35(5): 1081-1101.
[12]姜伟,王建国,靳玉荣,等. 设施土壤盐分变化规律及其相关分析研究[J]. 华北农学报, 2010, 25(2): 200-205.
[13]余海英,李廷轩,周健民. 设施土壤盐分的累积、迁移及离子组成变化特征[J]. 植物营养与肥料学报, 2007,13(4): 642-650.
[14]王瑞雪,徐智,汤利,等. 设施菜地土壤质量主要障碍因子及其修复措施研究进展[J]. 浙江农业科学, 2015, 56(8): 1300-1305.
[15]王秀红,王秀峰,杨凤娟,等. 外源精胺对NO-3胁迫下黄瓜幼苗抗氧化酶活性及光合作用的影响[J]. 植物营养与肥料学报, 2010, 16(4): 1020-1026.
[16]吴秀,陆晓民. 亚适宜温光盐环境下油菜素内酯对黄瓜幼苗抗氧化系统及光合作用的影响[J]. 应用生态学报, 2015, 26(9):2751-2757.
[17]束胜,孙锦,郭世荣,等. 外源腐胺对盐胁迫下黄瓜幼苗叶片PSⅡ光化学特性和体内离子分布的影响[J]. 园艺学报, 2010, 37(7): 1065-1072.
[18]李合生. 植物生理生化试验原理和技术[M]. 北京:高等教育出版社, 2000.
[19]陆晓民,孙锦,郭世荣,等. 油菜素内酯对低氧胁迫黄瓜幼苗根系线粒体抗氧化系统及其细胞超微结构的影响[J]. 园艺学报, 2012, 39(5): 888-896.
[20]PLUMB-DHINDSA P, THORPE T A. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of, superoxide dismutase and catalase[J]. Journal of Experimental Botany, 1981, 32(1): 93-101.
[21]王志泰,包玉. 土壤水分对大叶黄杨生长量与光合作用的影响[J]. 草业科学, 2010, 27(11): 95-100.
[22]李悦,宋士清,王久兴. 不同BR施用方式诱导黄瓜幼苗对Ca(NO3)2胁迫抗性的研究[J]. 西北植物学报, 2016, 36(2): 377-382.
[23]胡文海,师恺,曹玉林,等. 低温弱光对黄瓜幼苗地上部和根系生长与呼吸作用的影响[J]. 江西农业大学学报, 2008, 30(1): 15-19.
[24]高青海,王亚坤,陆晓民,等. 低温弱光下外源褪黑素对黄瓜幼苗生长及抗氧化系统的影响[J]. 西北植物学报, 2014, 34(8):1608-1613.
[25]张梦如,杨玉梅,成蕴秀,等. 植物活性氧的产生及其作用和危害[J]. 西北植物学报, 2014, 34(9): 1916-1926.
[26]陆晓民,杨威.油菜素内醋对氯化钠胁迫下黄瓜幼苗的缓解效应[J]. 应用生态学报, 2013, 24(5):1409-1414.
[27]BOROWSKI E, BLAMOWSKI Z K, MICHAEK W. Effects of tomatex /triacontanol/ on chlorophyll fluorescence and tomato ( Lycopersicon esculentum Mill.) yields[J]. Acta Physiologiae Plantarum, 2000, 22(3): 271-274.
[28]杨晓娟,赵璠,吕月玲. 三十烷醇对杏树光合作用的影响[J]. 西北林学院学报, 2012,27(3): 36-38.
[29]崔睿,刘喆,阚侃,等. 三十烷醇的生理作用及研究进展[J]. 黑龙江科学, 2011, 2(6):33-36.
[30]李雅洁,陆晓民. 腐胺对硝酸钙胁迫下黄瓜幼苗生长、抗氧化系统及光合作用的影响[J]. 北方园艺, 2017(3):5-10.
[31]王素平,王启明. 腐胺对弱光胁迫下黄瓜幼苗叶片SOD的调节[J]. 商丘师范学院学报, 2010, 26(12):87-89.
[32]徐向东,孙艳,孙波,等. 高温胁迫下外源褪黑素对黄瓜幼苗活性氧代谢的影响[J]. 应用生态学报, 2010, 21(10): 2580-2586.
[33]徐晓昀,郁继华,颉建明,等. 2,4-表油菜素内酯对亚适温弱光下黄瓜幼苗光合特性和抗氧化系统的影响[J].核农学报, 2017,31(5):979 -986.

相似文献/References:

[1]梁郸娜,胡其靖,曹 磊,等.蚜虫侵染对黄瓜叶片中丙二醛含量及保护酶活性的影响[J].江苏农业学报,2016,(02):278.[doi:10.3969/j.issn.1000-4440.2016.02.007]
 LIANG Dan-na,HU Qi-jing,CAO Lei,et al.Effects of aphid(Aphis gossypii Glover)infestation on MDA content and protective enzymes activities in cucumber[J].,2016,(02):278.[doi:10.3969/j.issn.1000-4440.2016.02.007]
[2]顾大路,王伟中,孙爱侠,等.不同轮作模式对日光温室黄瓜生长的影响[J].江苏农业学报,2016,(04):874.[doi:10.3969/j.issn.100-4440.2016.04.025]
 GU Da-lu,WANG Wei-zhong,SUN Ai-xia,et al.Cucumber growth in solar greenhouse affected by rotation modes[J].,2016,(02):874.[doi:10.3969/j.issn.100-4440.2016.04.025]
[3]马晓燕,高艳明,李建设.容器对基质栽培春茬黄瓜生长和产量的影响[J].江苏农业学报,2016,(04):879.[doi:10.3969/j.issn.100-4440.2016.04.026]
 MA Xiao-yan,GAO Yan-ming,LI Jian-she.Influences of containers on growth and yield of substrate-cultured spring cucumber[J].,2016,(02):879.[doi:10.3969/j.issn.100-4440.2016.04.026]
[4]张微微,潘俊松,蒋苏,等.黄瓜 RIL 群体侧枝相关性状 QTL 定位[J].江苏农业学报,2017,(01):174.[doi:10.3969/j.issn.1000-4440.2017.01.028]
 ZHANG Wei-wei,PAN Jun-song,JIANG Su,et al.Genetic mapping of QTL associated with lateral branch-related traits using recombinant inbred lines population in cucumber (Cucumis sativus L.)[J].,2017,(02):174.[doi:10.3969/j.issn.1000-4440.2017.01.028]
[5]徐心诚.弱光对温室黄瓜叶片和茎中可溶性糖含量的影响[J].江苏农业学报,2015,(06):1448.[doi:doi:10.3969/j.issn.1000-4440.2015.06.040]
 XU Xin-cheng.Soluble sugar contents in leaves and stems of greenhouse cucumber exposed to weak light[J].,2015,(02):1448.[doi:doi:10.3969/j.issn.1000-4440.2015.06.040]
[6]柯希欢,李霞,张琛,等.菇-热-酶-肥新型栽培方式对大棚黄瓜形态、生理和产量的影响[J].江苏农业学报,2015,(04):877.[doi:10.3969/j.issn.1000-4440.2015.04.026]
 KE Xi-huan,LI Xia,ZHANG Chen,et al.Effects of mushroom-heat-enzyme-fertilizer on the morphology, physiology and yield of greenhouse cucumber rotated with straw mushroom[J].,2015,(02):877.[doi:10.3969/j.issn.1000-4440.2015.04.026]
[7]李建设,刘宏久,郑佳琦,等.灌溉时间和灌水量对黄瓜穴盘苗生长及生理的影响[J].江苏农业学报,2015,(02):401.[doi:10.3969/j.issn.1000-4440.2015.02.028]
 LI Jian-she,LIU Hong-jiu,ZHENG Jia-qi,et al.Cucumber seedling growth and physiology influenced by irrigation time and amount[J].,2015,(02):401.[doi:10.3969/j.issn.1000-4440.2015.02.028]
[8]陈夕军,朱键鑫,陈羽,等.抗白粉病黄瓜品种的叶片组织结构及其生理生化[J].江苏农业学报,2015,(01):55.[doi:10.3969/j.issn.1000-4440.2015.01.008]
 CHEN Xi-jun,ZHU Jian-xin,CHEN Yu,et al.Physiological and biochemical mechanism of cucumber cultivars resistant to powdery mildew and their differences in microstructure of leaves[J].,2015,(02):55.[doi:10.3969/j.issn.1000-4440.2015.01.008]
[9]徐刚,彭天沁,高文瑞,等.氮肥水平对木薯渣复合基质栽培黄瓜生长及光合作用的影响[J].江苏农业学报,2015,(01):68.[doi:10.3969/j.issn.1000-4440.2015.01.010]
 XU Gang,PENG Tian-qin,GAO Wen-rui,et al.Effects of nitrogen fertilizer rates on growth and photosynthesis of cucumber in cassava compound substrate[J].,2015,(02):68.[doi:10.3969/j.issn.1000-4440.2015.01.010]
[10]曹玉杰,薄凯亮,程春燕,等.外源尸胺增强黄瓜幼苗耐冷性的生理机制[J].江苏农业学报,2015,(01):122.[doi:10.3969/j.issn.1000-4440.2015.01.019]
 CAO Yu-jie,BO Kai-liang,CHENG Chun-yan,et al.Physiological mechanism of exogenous cadaverine enhancing the chilling tolerance of cucumber seedlings[J].,2015,(02):122.[doi:10.3969/j.issn.1000-4440.2015.01.019]

备注/Memo

备注/Memo:
收稿日期:2017-08-20 基金项目:安徽省现代农业产业技术体系专项——蔬菜产业技术体系项目(AHCYTX-9);安徽高校省级自然科学研究项目(KJ2013Z040);校重点学科项目(AKZDXK2015C05) 作者简介:李雅洁(1992-),女,安徽省蚌埠人,硕士研究生,主要从事设施园艺及蔬菜生理生化研究。(E-mail)liyajie302@163.com 通讯作者:陆晓民, (E-mail)luxiaomin88@163.com
更新日期/Last Update: 2018-05-04