[1]陈珣,杨镇,曹君,等.人参内生菌提取物对硝酸盐胁迫下番茄幼苗生长及氮代谢相关指标的影响[J].江苏农业学报,2017,(05):1111-1116.[doi:doi:10.3969/j.issn.1000-4440.2017.05.023]
 CHEN Xun,YANG Zhen,CAO Jun,et al.Effects of extracts from Ginseng endophytic fungi on the growth and nitrogen metabolism of tomato seedlings under Ca(NO3)2 stress[J].,2017,(05):1111-1116.[doi:doi:10.3969/j.issn.1000-4440.2017.05.023]
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人参内生菌提取物对硝酸盐胁迫下番茄幼苗生长及氮代谢相关指标的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
1111-1116
栏目:
园艺
出版日期:
2017-10-30

文章信息/Info

Title:
Effects of extracts from Ginseng endophytic fungi on the growth and nitrogen metabolism of tomato seedlings under Ca(NO3)2 stress
作者:
陈珣杨镇曹君刘国丽龚娜
(辽宁省农业科学院食用菌研究所,辽宁沈阳110161)
Author(s):
CHEN XunYANG ZhenCAO JunLIU Guo-liGONG Na
(Institute of Edible Fungi, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China)
关键词:
番茄幼苗硝酸盐胁迫人参内生菌提取物氮代谢
Keywords:
tomato seedlingsCa(NO3)2 stressextracts from Ginseng endophytenitrogen metabolism
分类号:
S641.2;X53
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.023
文献标志码:
A
摘要:
以番茄幼苗为试验材料,研究不同浓度(10 μg/ml、5 μg/ml和1 μg/ml)的人参内生菌(Alternaria sp.)提取物在Ca(NO3)2胁迫(75 mmol/L)下对番茄幼苗生长及氮代谢相关指标的影响。结果表明,与清水对照相比,硝酸盐胁迫处理下番茄幼苗生长受到抑制,可溶性蛋白含量、脯氨酸含量、硝态氮含量和氮代谢相关酶活性均受到影响。人参内生菌提取物浓度为5 μg/ml的处理明显缓解了硝酸盐胁迫对番茄幼苗生长造成的伤害,显著提高了可溶性蛋白含量、硝酸还原酶(NR)活性、谷氨酸合成酶(GOGAT)活性和谷氨酸脱氢酶(GDH)活性,并显著降低了脯氨酸含量,说明5 μg/ml人参内生菌提取物处理对硝酸盐胁迫下番茄幼苗生长和氮代谢的调节效果最佳。
Abstract:
Three different concentrations of extracts from Ginseng endophyte were used to treat tomato seedlings, and the effects of extracts from Ginseng endophyte on the growth and nitrogen metabolism of tomato seedlings under Ca(NO3)2 stress were studied. The results showed that the growth of tomato seedlings under Ca(NO3)2 stress was inhibited, the soluble protein content, proline content, nitrate nitrogen content and the activity of enzymes related to nitrogen metabolism were all affected, compared with the control group. 5 μg/ml of extracts from endophyte could alleviate the injury of plant caused by Ca(NO3)2 stress, significantly improve the soluble protein content, NR activity, GOGAT activity and GDH activity, and significantly reduc the content of proline. This suggested that the treatment of irrigating 5 μg/ml endophyte extracts showed the best effects on the growth and nitrogen metabolism of tomato seedlings under nitrate stress.

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备注/Memo

备注/Memo:
收稿日期:2017-07-10 基金项目:辽宁省科学事业公益基金项目(2015002006);沈阳市科技攻关项目(17163300) 作者简介:陈珣(1979-),女,辽宁沈阳人,硕士,助理研究员,主要从事农业微生物方面的研究。(Tel) 13840424970;(E-mail)chenxun_82_ren@126.com 通讯作者:龚娜,(E-mail)doll52133@163.com
更新日期/Last Update: 2017-11-03