[1]崔凯,葛静,柴阳阳,等.噻虫嗪对水稻内生真菌群落组成的影响[J].江苏农业学报,2017,(04):802-808.[doi:doi:10.3969/j.issn.1000-4440.2017.04.012]
　CUI Kai,GE Jing,CHAI Yang-yang,et al.Changes of endophytic fungal community in rice plants exposed to thiamethoxam[J].,2017,(04):802-808.[doi:doi:10.3969/j.issn.1000-4440.2017.04.012]
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噻虫嗪对水稻内生真菌群落组成的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年04期
页码:
802-808
栏目:
植物保护
出版日期:
2017-08-30

文章信息/Info

Title:
Changes of endophytic fungal community in rice plants exposed to thiamethoxam
作者:
崔凯1; 2; 葛静2; 柴阳阳1; 2; 杨丽璇2; 程江峰1; 余向阳2
（1.青岛科技大学化工学院，山东青岛266042；2.江苏省农业科学院食品质量安全与检测研究所，江苏南京210014）
Author(s):
CUI Kai1; 2; GE Jing2; CHAI Yang-yang1; 2; YANG Li-xuan2; CHENG Jiang-feng1; YU Xiang-yang2
(1.College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;2.Institute of Food Safety and Inspection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
噻虫嗪; 水稻; 内生真菌; 高通量测序
Keywords:
thiamethoxam; rice; endophytic fungi; high-throughput sequencing
分类号:
S511.2+2
DOI:
doi:10.3969/j.issn.1000-4440.2017.04.012
文献标志码:
A
摘要:
为了研究噻虫嗪胁迫对水稻内生真菌的影响，采用室内盆栽、土壤处理的方法，利用Illumina MiSeq高通量测序技术分析了水稻根、茎和叶的内生真菌群落变化。噻虫嗪土壤处理浓度为0 mg/kg、1 mg/kg、10 mg/kg，分别标记为CK、FR、10×FR。本研究获得用于分析的优质序列和操作分类单元（OTU）数分别为757 058和456。α多样性指数分析结果表明，水稻根部真菌多样性随噻虫嗪处理浓度的增加而增加，而其茎、叶的真菌多样性只在高浓度噻虫嗪处理下增加，低浓度处理下则无明显变化。在属的水平上对代表性OTU进行注释，发现水稻根、茎、叶中内生真菌组成受噻虫嗪影响发生了明显变化。表明噻虫嗪对水稻内生真菌群落组成的影响显著。
Abstract:
A pot experiment was carried out to study the impact of thiamethoxam on endophytic fungal community changes in rice roots, stems and leaves by Illumina MiSeq high-throughput sequencing technique. Three levels of thiamethoxan were applied， 0 (CK), 1 mg/kg (FR), 10 mg/kg (10×FR). Totally 757 058 effective sequences and 456 operational taxonomic units (OTUs) were identified. Alpha diversity index revealed that the fungal diversities were getting higher as the concentration of thiamethoxam went up. However, the fungal diversities of rice stems and leaves increased only when exposed to high concentration of thiamethoxam. The annotation of representative OTU showed that thiamethoxam altered the structure of endophytic fungal communities in roots, stems and leaves of rice at the genus level.

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备注/Memo

备注/Memo:
收稿日期：2017-03-16 基金项目：国家自然科学基金项目（31601660、31572032）;江苏省农业自主创新基金项目[CX(14)2060] 作者简介：崔凯（1991-）,男,山东临沂人，硕士研究生，主要从事微生物多样性研究工作。（Tel）15895845930；（E-mail）ck1991ly@163.com 通讯作者：余向阳,(E-mail）yuxy@jaas.ac.cn
更新日期/Last Update: 2017-09-01