[1]刘爱平,申文浩,王小红,等.免疫学检测中抗体固定化方法的研究现状[J].江苏农业学报,2017,(03):714-720.[doi:doi:10.3969/j.issn.1000-4440.2017.03.034]
　LIU Ai-ping,SHEN Wen-hao,WANG Xiao-hong,et al.A review on antibody immobilization in immunoassays[J].,2017,(03):714-720.[doi:doi:10.3969/j.issn.1000-4440.2017.03.034]
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免疫学检测中抗体固定化方法的研究现状()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年03期
页码:
714-720
栏目:
综述
出版日期:
2017-06-30

文章信息/Info

Title:
A review on antibody immobilization in immunoassays
作者:
刘爱平1; 申文浩1; 王小红2; 李诚1
（1.四川农业大学食品学院，四川雅安625014；2.华中农业大学食品学院，湖北武汉430070）
Author(s):
LIU Ai-ping 1; SHEN Wen-hao 1; WANG Xiao-hong 2; LI Cheng 1
(1.College of Food Science, Sichuan Agricultural University, Yaan 625014， China;2.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070， China)
关键词:
抗体; 定向固定; 生物素-亲和素; 亲和肽配基; DNA引导固定法
Keywords:
antibody; oriented-immobilization; avidin-biotin; affinity peptide ligand; DNA-directed immobilization
分类号:
Q599
DOI:
doi:10.3969/j.issn.1000-4440.2017.03.034
文献标志码:
A
摘要:
免疫学检测技术，因具有快速、灵敏、成本低等优点，被广泛应用于食品安全、环境监测等领域。抗体是免疫学检测的核心试剂，免疫学检测中常需要对抗体进行固定，高效固定抗体对提高检测灵敏度具有重要意义。本文对抗体固定化各类方法的特点及其在免疫学检测中的应用进行综述，并对抗体定向固定化技术发展趋势进行了展望。
Abstract:
Immunological assays are widely applied in food safety and environmental monitoring because they are rapid, sensitive and of low price. Antibody, the core reagent in immunological assays, usually needs to be immobilized to capture antigen. It is of great significance to improve detection sensitivity through effective immobilization of antibody. This review seeks to overview the characteristics and application of each kind of immobilization methods in immunological assays, and the developing trends of oriented-immobilization technique were prospected.

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备注/Memo

备注/Memo:
收稿日期：2016-08-22 基金项目：国家自然科学基金项目（ 31271876）；中央高校基本科研业务费专项（2013PY103、52209-814012） 作者简介：刘爱平(1986-)，男，湖北荆州人，博士，讲师，主要从事食品微生物与免疫学检测研究，（ E-mail）aipliu@outlook.com 通讯作者：李诚， （ E-mail）lichenglcp@163.com
更新日期/Last Update: 2017-06-29