[1]闫乐艳,GEORGE Mann,施振旦.利用Onapristone研究绵羊子宫内膜组织中孕酮对PGF2α分泌以及COX-2表达的影响[J].江苏农业学报,2017,(03):624-629.[doi:doi:10.3969/j.issn.1000-4440.2017.03.020]
 YAN Le-yan,GEORGE Mann,SHI Zhen-dan.Regulation of progesterone in PGF2α secretion and COX-2 expression by onapristone in ovine endometrial cells[J].,2017,(03):624-629.[doi:doi:10.3969/j.issn.1000-4440.2017.03.020]
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利用Onapristone研究绵羊子宫内膜组织中孕酮对PGF2α分泌以及COX-2表达的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年03期
页码:
624-629
栏目:
畜牧兽医·水产养殖
出版日期:
2017-06-30

文章信息/Info

Title:
Regulation of progesterone in PGF2α secretion and COX-2 expression by onapristone in ovine endometrial cells
作者:
闫乐艳1GEORGE Mann 2施振旦1
(1.江苏省农业科学院畜牧研究所,江苏南京210014; 2.诺丁汉大学生物学院,英国拉夫堡LE12RD)
Author(s):
YAN Le-yan1GEORGE Mann 2SHI Zhen-dan1
(1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2. School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UK)
关键词:
孕酮受体抑制剂Onapristone前列腺素2α(PGF2α)环氧合酶-2(COX-2)孕酮绵羊
Keywords:
progesterone receptor inhibitor(onapristone)prostaglandin(PGF2α)cyclooxygenase(COX-2)progesteronesheep
分类号:
S858.26
DOI:
doi:10.3969/j.issn.1000-4440.2017.03.020
文献标志码:
A
摘要:
为了探讨孕酮通过环氧合酶-2(COX-2)/ 前列腺素2α(PGF2α)信号通路调节家畜黄体溶解的作用机制,本研究采用孕酮受体的特异抑制剂Onapristone处理体外培养的绵羊子宫内膜组织,检测分泌到培养液中的PGF2α浓度以及子宫内膜中PGF2α合成过程中关键酶基因COX-2的mRNA和蛋白质表达水平。结果显示,Onapristone 添加到子宫内膜组织培养液中后,内膜组织合成分泌的PGF2α浓度明显下降,且抑制了COX-2 mRNA和蛋白质表达水平,高剂量的Onapristone(200 μmol/L)处理72 h后COX-2的蛋白表达水平仅为对照组的23%。表明,孕酮通过其受体介导的COX-2/ PGF2α信号通路调节家畜的黄体溶解过程,且COX-2对于PGF2α的合成起到重要的作用。
Abstract:
To explore the regulatory role of progesterone in luteolysis via cyclooxygenase-2(COX-2) /prostaglandin F2α (PGF2α) signal pathway in livestock, the ovine endometrium explants were cultured and treated with specific inhibitor of progesterone receptor (onapristone). The PGF2α concentration and mRNA and protein expression levels of PGF2α synthesis-related enzyme COX-2 were detected. The applications of onapristone (02 μmol/L, 20 μmol/L or 200 μmol/L) in ovine endometrium culture medium inhibited the PGF2α secretion, especially when the explants were cultured for 72 h in the presence of 200 μmol/L onapristone (P<005). In addition, the mRNA expression of COX-2 was suppressed in endometrial explants cultured with onapristone for 24 h, 48 h and 72 h (P<001). The endometrial COX-2 protein expression was also downregulated significantly after 200 μmol/L onapristone treatment for 72 h, only about 23% of the control. In conclusion, progesterone regulates luteolysis via progesterone receptor/ COX-2 / PGF2α signal pathway in sheep, and COX-2 seems to be the primary enzyme responsible for PGF2α production.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-02-28 基金项目:国家自然科学基金项目(31601943) 作者简介:闫乐艳(1984-),女,山东济宁人,博士,助理研究员,主要从事家畜遗传育种与繁殖方面的研究。(Tel)025-84390336;(E-mail)yanleyan198469@126.com 通讯作者:施振旦,(Tel)025-84390956;(E-mail)zdshi@jaas.ac.cn
更新日期/Last Update: 2017-06-29