[1]马娜,王金彦,阚家亮,等.一个响应盐胁迫的梨凝集素受体蛋白激酶PcLRLK066 的功能分析[J].江苏农业学报,2017,(02):404-411.[doi:doi:10.3969/j.issn.1000-4440.2017.02.026]
　MA Na,WANG Jin-yan,KAN Jia-liang,et al.Functional analysis of a salt-induced lectin receptor-like kinase PcLRLK066 of pear[J].,2017,(02):404-411.[doi:doi:10.3969/j.issn.1000-4440.2017.02.026]
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一个响应盐胁迫的梨凝集素受体蛋白激酶PcLRLK066 的功能分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年02期
页码:
404-411
栏目:
园艺
出版日期:
2017-04-30

文章信息/Info

Title:
Functional analysis of a salt-induced lectin receptor-like kinase PcLRLK066 of pear
作者:
马娜12; 王金彦3; 阚家亮2; 蔺经2; 常有宏12
(1.南京农业大学园艺学院，江苏南京210095；2.江苏省农业科学院园艺研究所，江苏南京210014；3.江苏省农业科学院农业生物技术研究所，江苏南京210014)
Author(s):
MA Na12; WANG Jin-yan3; KAN Jia-liang2; LIN Jing2; CHANG You-hong12
(1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China；2.Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；3.Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
凝集素受体蛋白激酶; 膜定位蛋白; 非生物胁迫
Keywords:
LecRLK; membrane protein; abiotic stress
分类号:
S661.2;Q783
DOI:
doi:10.3969/j.issn.1000-4440.2017.02.026
文献标志码:
A
摘要:
为了探讨梨凝集素受体蛋白激酶(LecRLK)对非生物胁迫(盐、渗透胁迫)的响应情况，以梨PcLRLK066基因为研究对象，研究LRK066在梨和其他物种间的进化关系及其在豆梨不同组织中的表达情况，并观察了其在细胞中的定位情况，此外还研究了转PcLRLK066 拟南芥在不同盐浓度和甘露醇浓度处理下的发芽情况。结果显示，豆梨中PcLRLK066 与苹果进化距离最近，与野生番茄最远；PcLRLK066 在不同组织器官中表达差异显著；通过亚细胞定位发现PcLRLK066可能定位在细胞膜上；转入PcLRLK066 基因后的拟南芥在盐和渗透胁迫处理下发芽率均低于野生型拟南芥，表明转基因拟南芥对盐和干旱更加敏感，PcLRLK066 可能参与了盐胁迫和渗透胁迫。
Abstract:
To explore the role of pear LecRLK in response to abiotic stress such as salt and osmotic stress, the evolutionary relationship of PcLRLK066 in pear and other species were studied. The phylogenetic tree showed that PcLRLK066 in pear and apple had the nearest evolutionary distance to apple but the farthest to while wild tomato. PT-PCR revealed that PcLRLK066 were differentially expressed in different organs of pear. PcLRLK066 was subcellularly localized on plasma membrane. The germination rates of transgenic Arabidopsis plants under salt and osmotic stress were lower than those in wild type Arabidopsis plants, indicating that PcLRLK066-transgenic Arabidopsis is more sensitive to abiotic stress and PcLRLK066 might be involved in the responses.

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备注/Memo

备注/Memo:
收稿日期：2016-07-11 基金项目：国家自然科学基金项目（31372051）；江苏省农业科技自主创新基金项目[CX(14)5018] 作者简介：马娜（1989-），河北唐山人，硕士研究生，主要从事果树逆境生理和分子生物学研究。（E-mail）mn506381141@163.com 通讯作者：常有宏，（E-mail）cyh@jaas.ac.cn
更新日期/Last Update: 2017-05-02