[1]张微微,潘俊松,蒋苏,等.黄瓜 RIL 群体侧枝相关性状 QTL 定位[J].江苏农业学报,2017,(01):174-180.[doi:10.3969/j.issn.1000-4440.2017.01.028]
　ZHANG Wei-wei,PAN Jun-song,JIANG Su,et al.Genetic mapping of QTL associated with lateral branch-related traits using recombinant inbred lines population in cucumber (Cucumis sativus L.)[J].,2017,(01):174-180.[doi:10.3969/j.issn.1000-4440.2017.01.028]
点击复制

黄瓜 RIL 群体侧枝相关性状 QTL 定位()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年01期
页码:
174-180
栏目:
园艺
出版日期:
2017-02-28

文章信息/Info

Title:
Genetic mapping of QTL associated with lateral branch-related traits using recombinant inbred lines population in cucumber (Cucumis sativus L.)
作者:
张微微1; 2; 潘俊松2; 蒋苏2; 何欢乐2; 俞平高1; 蔡润2
(1.上海农林职业技术学院园艺园林系，上海201699；2.上海交通大学农业与生物学院，上海200240)
Author(s):
ZHANG Wei-wei1; 2; PAN Jun-song2; JIANG Su2; HE Huan-le2; YU Ping-gao1; CAI Run2
(1.Horticulture and Landscape College, Shanghai Vocational College of Agriculture and Forestry, Shanghai 201699, China;2.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China)
关键词:
黄瓜; 遗传图谱; 侧枝; QTL
Keywords:
cucumber; genetic map; lateral branch; QTL
分类号:
S642.2
DOI:
10.3969/j.issn.1000-4440.2017.01.028
文献标志码:
A
摘要:
利用侧枝长势弱、萌发早的华北类型品系S94和侧枝长势强、萌发晚的欧洲类型品系S06构建的224个F6:7代重组自交系(RIL)群体，对春、秋两季F7黄瓜侧枝相关性状表型进行研究。利用重组自交系群体构建遗传图谱，采用WinQTLcart2.5软件复合区间作图法，对4个侧枝相关性状[侧枝总长(lbtl)、侧枝均长(lbal)、侧枝数目(lbn)和第一侧枝节位(flbn)] QTL位点进行检测。结果显示，4个侧枝相关性状在秋、春两季共检测到45个QTLs位点(秋季29个，春季16个)，分布于全部7条染色体上。在侧枝相关性状中，春、秋两季相同性状都能稳定检测到的QTL有8个，其中2个QTL侧枝均长QTL lbal1.1（秋季贡献率16.4%，春季贡献率13.5%）和侧枝总长QTL lbtl1.1(秋季贡献率21.2%，春季贡献率26.9%)在两季的贡献率都高于10%，其余位点的贡献率(>10%)都在单一季节表现。
Abstract:
A set of 224 recombinant inbred lines (RILs) were derived from a cross between S94 (Northern China type with weak lateral branch growth potential and early lateral branch sprouting time) and S06 (Northern European type with strong lateral branch growth potential and late lateral branch sprouting time). The phenotypic data in different seasons (F7 in spring and F7 in autumn)were used to identify the quantitative trait loci for four lateral branch-related traits (lateral branch total length (lbtl), lateral branch average length (lbal), lateral branch number (lbn), and first lateral branch node (flbn)) using WinQTLcart2.5 software.The results showed a total of 45 QTLs for lateral branch-related traits were detected in F7 in autumn (29) and spring (16), and the QTLs distributed in all seven chromosomes. Of all lateral branch-related traits, QTLs of same traits detected both in spring and autumn were eight, of which lateral branch average length lbal1.1 (16.4% in autumn, 13.5% in spring) and lateral branch total length lbal1.1 (21.2% in autumn, 26.9% in spring) had stable position and higher contribution in both seasons, and the rest of loci showed high contribution (> 10%) in a single season.

参考文献/References:

[1]SERQUEN F C, BACHER J, STAUB J E. Mapping and QTL analysis of horticultural traits in a narrow cross in cucumber (Cucumissativas L.) using random amplified polymorphic DNA makers [J]. Molecular Breeding, 1997, 3(4): 257-268.
[2]FAZIO G, STAUB J E. Genetic mapping and QTL analysis of horticultural traits in cucumber (Cucumissativus L.) using recombinant inbred lines [J]. Theoretical and Applied Genetics, 2003, 107: 864-874.
[3]LI X Z, PAN J S,WANG G, et al. Localization of genes for lateral branch and female sex expression and construction of a molecular linkage map in cucumber (Cucumissativus L.) with RAPD markers [J]. Progress in Natural Science, 2005, 15(2): 143-148.
[4]WANG G, PAN J S, LI X Z, et al. Construction of a cucumber genetic linkage map with SRAP markers and location of the gene for lateral branch traits [J]. Science in China Ser. C: Life Sciences, 2005, 48 (3): 213-220.
[5]JIANG S, YUAN X J, PAN J S, et al. Quantitative trait locus analysis of lateral branch-related traits in cucumber (Cucumissativus L.) using recombinant inbred lines [J]. Science in China Series C: Life Sciences, 2008, 51(9):833-841.
[6]YUAN X J, LI X Z, PAN J S, et al. Genetic linkage map construction and location of QTLs for fruit-related traits in cucumber [J]. Plant Breeding, 2008a, 127(2): 180-188.
[7]YUAN X J, PAN J S, CAI R, et al.Genetic mapping and QTL analysis of fruit and flower related traits in cucumber (CucumissativusL.) using recombinant inbred lines [J]. Euphytica, 2008b, 164:473-491.
[8]袁晓君. 黄瓜永久群体遗传图谱的构建及花、果实相关性状的QTL定位[D]. 上海:上海交通大学，2008.
[9]MOHAN RH Y, SETT R. Induction of fertile male flower in genetically female Cannabis sativa plants by silver nitrate and silver thiosulphate anionic complex[J]. Theoretical and Applied Genetics, 1982, 62: 369-375.
[10]ZHANG W W, PAN J S, HE H L, et al. Construction of a high-density integrated genetic linkage map for cucumber (CucumissativusL.) [J]. Theor Appl Genet, 2012, 124(2):249-259.
[11]KONG Q, XIANG C, YU Z, et al.Mining and charactering microsatellites in Cucumismelo expressed sequence tags from sequence database[J]. Molecular Ecology Notes, 2007, 7: 281-283.
[12]CHIBA N, SUWABE K, NUNOME T, et al. Development of microsatellite markers in melon (Cucumismelo L.) and their application to major cucurbit crops[J]. Breeding Science, 2003, 53: 21-27.
[13]WATCHARAWONGPAIBOON N, CHUNWONGSE J. Development and characterization of microsatellite markers from an enriched genomic library of cucumber (Cucumissativus)[J]. Plant Breeding, 2007, 127: 74-81.
[14]朱正歌，贾继增，孙宗修. AFLP 指纹银染法显带研究[J]. 中国水稻科学, 2002, 16(1): 71-73.
[15]VAN OOIJEN J W, VOORRIPS R E. JoinMap3.0: Software for the calculation of genetic linkage maps[M].Wageningen, The Netherlands: Plant Research International BV, 2002. 
[16]WANG S, BASTEN C J, ZENG Z B. Windows QTL cartographer 2.5. department of statistics[M].Raleigh, NC: North Carolina State University, 2006.
[17]REN Y, ZHANG Z, LIU J, et al.An integrated genetic and cytogenetic map of the cucumbergenome[J]. PloS One, 2009, 4(6): 5795.
[18]LU H, ROMERO-SEVERSON J, BERNARDO R. Chromosomal regions associated with segregation distortion in maize[J]. Theoretical and Applied Genetics, 2002, 105: 622-628.
[19]MATSUSHITA S, ISEKI T, FUKUTA Y, et al. Characterization of segregation distortion on chromosome 3 induced in wide hybridization between indica and japonica type rice varieties[J]. Euphytica, 2003, 134: 27-32.[20]VOGL C, XU S. Multipoint mapping of viability and segregation distorting loci using molecular markers[J]. Genetics, 2000, 155: 1439-1447.
[21]XU Y, ZHU L, XIAO J, et al. Chromosomal regions associated with segregation distortion of molecular markers in F2,backcross,double haploid,and recombinant inbred populations in rice (Oryza sativa L.)[J]. Molecular Genetics and Genomics, 1997, 253(5):535-545.
[22]FAN Z H, ROBBINS M D, STAUB J E. Population development by phenotypic selection with subsequent marker-assisted selection for line extraction in cucumber (CucumissativusL.)[J]. Theoretical and Applied Genetics, 2006, 112(5): 843-855.
[23]LECOMTE L, DUFFE P, BURET M, et al. Marker-assisted introgression of five QTLs controlling fruit quality traits into three tomato lines revealed interactions between QTLs and genetic backgrounds[J].Theoretical and Applied Genetics, 2004, 109(3): 658-668.
[24]ZHU B G, SUN Y R. Inheritance of the four-seeded-pod trait in a soybean mutant and marker-assisted selection for this trait[J]. Plant Breeding, 2006, 125: 405-407.
[25]ASHIKARI M, SAKAKIBARA H, LIN S Y, et al.Cytokinin oxidase regulates rice grain production[J]. Science, 2005, 309(5735): 741-745.
[26]CLARK R M, WAGLER T N, QUIJADA P, et al.A distant upstream enhancer at the maize domestication gene tb1 has pleiotropic effects on plant and inflorescent architecture[J]. Nature Genetics, 2006, 38(5):594-597.

相似文献/References:

[1]梁郸娜,胡其靖,曹 磊,等.蚜虫侵染对黄瓜叶片中丙二醛含量及保护酶活性的影响[J].江苏农业学报,2016,(02):278.[doi:10.3969/j.issn.1000-4440.2016.02.007]
　LIANG Dan-na,HU Qi-jing,CAO Lei,et al.Effects of aphid(Aphis gossypii Glover)infestation on MDA content and protective enzymes activities in cucumber[J].,2016,(01):278.[doi:10.3969/j.issn.1000-4440.2016.02.007]
[2]顾大路,王伟中,孙爱侠,等.不同轮作模式对日光温室黄瓜生长的影响[J].江苏农业学报,2016,(04):874.[doi:10.3969/j.issn.100-4440.2016.04.025]
　GU Da-lu,WANG Wei-zhong,SUN Ai-xia,et al.Cucumber growth in solar greenhouse affected by rotation modes[J].,2016,(01):874.[doi:10.3969/j.issn.100-4440.2016.04.025]
[3]马晓燕,高艳明,李建设.容器对基质栽培春茬黄瓜生长和产量的影响[J].江苏农业学报,2016,(04):879.[doi:10.3969/j.issn.100-4440.2016.04.026]
　MA Xiao-yan,GAO Yan-ming,LI Jian-she.Influences of containers on growth and yield of substrate-cultured spring cucumber[J].,2016,(01):879.[doi:10.3969/j.issn.100-4440.2016.04.026]
[4]徐心诚.弱光对温室黄瓜叶片和茎中可溶性糖含量的影响[J].江苏农业学报,2015,(06):1448.[doi:doi:10.3969/j.issn.1000-4440.2015.06.040]
　XU Xin-cheng.Soluble sugar contents in leaves and stems of greenhouse cucumber exposed to weak light[J].,2015,(01):1448.[doi:doi:10.3969/j.issn.1000-4440.2015.06.040]
[5]柯希欢,李霞,张琛,等.菇-热-酶-肥新型栽培方式对大棚黄瓜形态、生理和产量的影响[J].江苏农业学报,2015,(04):877.[doi:10.3969/j.issn.1000-4440.2015.04.026]
　KE Xi-huan,LI Xia,ZHANG Chen,et al.Effects of mushroom-heat-enzyme-fertilizer on the morphology, physiology and yield of greenhouse cucumber rotated with straw mushroom[J].,2015,(01):877.[doi:10.3969/j.issn.1000-4440.2015.04.026]
[6]李建设,刘宏久,郑佳琦,等.灌溉时间和灌水量对黄瓜穴盘苗生长及生理的影响[J].江苏农业学报,2015,(02):401.[doi:10.3969/j.issn.1000-4440.2015.02.028]
　LI Jian-she,LIU Hong-jiu,ZHENG Jia-qi,et al.Cucumber seedling growth and physiology influenced by irrigation time and amount[J].,2015,(01):401.[doi:10.3969/j.issn.1000-4440.2015.02.028]
[7]陈夕军,朱键鑫,陈羽,等.抗白粉病黄瓜品种的叶片组织结构及其生理生化[J].江苏农业学报,2015,(01):55.[doi:10.3969/j.issn.1000-4440.2015.01.008]
　CHEN Xi-jun,ZHU Jian-xin,CHEN Yu,et al.Physiological and biochemical mechanism of cucumber cultivars resistant to powdery mildew and their differences in microstructure of leaves[J].,2015,(01):55.[doi:10.3969/j.issn.1000-4440.2015.01.008]
[8]徐刚,彭天沁,高文瑞,等.氮肥水平对木薯渣复合基质栽培黄瓜生长及光合作用的影响[J].江苏农业学报,2015,(01):68.[doi:10.3969/j.issn.1000-4440.2015.01.010]
　XU Gang,PENG Tian-qin,GAO Wen-rui,et al.Effects of nitrogen fertilizer rates on growth and photosynthesis of cucumber in cassava compound substrate[J].,2015,(01):68.[doi:10.3969/j.issn.1000-4440.2015.01.010]
[9]曹玉杰,薄凯亮,程春燕,等.外源尸胺增强黄瓜幼苗耐冷性的生理机制[J].江苏农业学报,2015,(01):122.[doi:10.3969/j.issn.1000-4440.2015.01.019]
　CAO Yu-jie,BO Kai-liang,CHENG Chun-yan,et al.Physiological mechanism of exogenous cadaverine enhancing the chilling tolerance of cucumber seedlings[J].,2015,(01):122.[doi:10.3969/j.issn.1000-4440.2015.01.019]
[10]张善文,谢泽奇,张晴晴.卷积神经网络在黄瓜叶部病害识别中的应用[J].江苏农业学报,2018,(01):56.[doi:doi:10.3969/j.issn.1000-4440.2018.01.008]
　ZHANG Shan-wen,XIE Ze-qi,ZHANG Qing-qing.Application research on convolutional neural network for cucumber leaf disease recognition[J].,2018,(01):56.[doi:doi:10.3969/j.issn.1000-4440.2018.01.008]

备注/Memo

备注/Memo:
收稿日期：2016-05-17基金项目：上海市自然科学基金项目（15ZR1429800）作者简介：张微微（1981-），女，黑龙江鸡西人，博士研究生，高级工程师，主要从事黄瓜分子育种。（Tel）13381798786;（E-mail）zhangww@shafc.edu.cn通讯作者：蔡润，（Tel）021-34206938;（E-mail）cairun@sjtu.edu.cn
更新日期/Last Update: 2017-04-12