[1]赵桂华,刘国华,赵楠.中国铁皮石斛茎腐病的病原鉴定[J].江苏农业学报,2016,(04):780-785.[doi:10.3969/j.issn.100-4440.2016.04.011]
　ZHAO Gui-hua,LIU Guo-hua,ZHAO Nan.Pathogen identification of stem rot disease in Dendrobium officinale of China[J].,2016,(04):780-785.[doi:10.3969/j.issn.100-4440.2016.04.011]
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中国铁皮石斛茎腐病的病原鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
780-785
栏目:
植物保护
出版日期:
2016-10-31

文章信息/Info

Title:
Pathogen identification of stem rot disease in Dendrobium officinale of China
作者:
赵桂华; 刘国华; 赵楠
江苏农林职业技术学院，江苏 句容 212400
Author(s):
ZHAO Gui-hua; LIU Guo-hua; ZHAO Nan
Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400, China
关键词:
铁皮石斛; 可可球二孢; 致病性; 发生规律
Keywords:
Dendrobium officinale;  Lasiodiplodia theobromae;  pathogenicity; occurrence regularity
分类号:
S567.23
DOI:
10.3969/j.issn.100-4440.2016.04.011
文献标志码:
A
摘要:
为了研究铁皮石斛茎腐病的发生与防治，2013年4月至2015年8月，观察了铁皮石斛茎腐病症状、并进行了病原菌分离、致病性试验和病原菌鉴定。结果表明，该病害仅为害铁皮石斛茎秆，严重发生时，可导致病斑以上的茎秆死亡；TP-1菌株分离得率可达65.7%；接种病原菌14 d检查全部发病，出现水渍状坏死病斑，直径约3 mm，症状与自然发病相似，再分离得到了原菌株。经形态学和DNA分子测序鉴定，确定TP-1为可可球二孢，它是引起铁皮石斛茎腐病的主要病原菌。这由可可球二孢造成铁皮石斛的茎腐病，属国内外首次报道。
Abstract:
For the control of stem rot disease in Dendrobium afficinale, the symptoms were observed, pathogen was isolated and identified in April 2013 to August 2015. The pathogen only infected the stem of D. officinale，resulting in the stem death above diseased spot. Strain TP-1 accounted for 65.7% of the isolated fungi colonies. After inoculation of TP-1 for 14 d, watery lesions about 3 mm in diameter occurred, which was similar to natural infections, and original strain was re-isolated. Morphological and sequencing identified that TP-1 is Lasiodiplodia theobromae, This is the first report of stem rot disease in D. afficinalecaused by L. theobromae in China.

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备注/Memo

备注/Memo:
收稿日期：2015-12-21基金项目：江苏农林职业技术学院科技创新团队项目(2015kj036)；江苏省科技支撑计划项目(BE2014385)作者简介：赵桂华（1952-），男，山东费县人，本科，教授，主要从事植物病害研究。(E-mail)1723686916@qq.com
更新日期/Last Update: 2016-11-01