[1]李强子,朱国强,刘吴鑫,等.荷斯坦牛TLR6基因CDS区的生物信息学分析[J].江苏农业学报,2016,(03):608.[doi:10.3969/j.issn.1000-4440.2016.03.019]
　LI Qiang-zi,ZHU Guo-qiang,LIU Wu-xin,et al.Bioinformatics analysis of CDS of TLR6 gene in Holstein cattle[J].,2016,(03):608.[doi:10.3969/j.issn.1000-4440.2016.03.019]
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荷斯坦牛TLR6基因CDS区的生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
608
栏目:
畜牧兽医·水产养殖
出版日期:
2016-06-30

文章信息/Info

Title:
Bioinformatics analysis of CDS of TLR6 gene in Holstein cattle
作者:
李强子;  朱国强;  刘吴鑫;  刘丽霞;  张 丽
西北民族大学生命科学与工程学院,甘肃 兰州 730030
Author(s):
LI Qiang-zi;  ZHU Guo-qiang;  LIU Wu-xin;  LIU Li-xia;  ZHANG Li
College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, China
关键词:
荷斯坦牛;  TLR6基因;  生物信息学
Keywords:
Holstein cattle;  TLR6 gene;  bioinformatics
分类号:
S823.2
DOI:
10.3969/j.issn.1000-4440.2016.03.019
文献标志码:
A
摘要:
采用生物信息学方法分析和预测了荷斯坦牛TLR6基因编码蛋白质的理化特性、结构功能及其同源进化关系。结果表明,荷斯坦牛TLR6基因编码793个氨基酸,组成蛋白为不稳定的水溶性蛋白,二级结构为混合型,三级结构由α-螺旋、β-折叠、β-转角和无规则卷曲组成。蛋白质功能显示信号转导、胁迫应答、免疫应答和生长因子的几率相对较高。荷斯坦牛TLR6基因氨基酸序列与牦牛的同源性较高。说明TLR6基因能在荷斯坦牛免疫应答和生长调节过程中发挥重要作用,可能对牛的抗病力和生产性能起作用。
Abstract:
In order to provide a theoretical basis for disease resistance molecular breeding and genetic screening of economic traits of cattle, the physical and chemical properties, the structure and function of the encoded product and homologous evolutionary relationships of TLR6 gene in Holstein cattle were analyzed and predicted by means of bioinformatics. TLR6 gene encodes 793 amino acids. The TLR6 gene-encoded protein is a kind of instable and soluble protein, with mixed-type secondary structure and tertiary structure consisting of alpha helix, extended strand, beta turn and random coil. The TLR6 protein was predicted to play a major role in signal transduction, stress response, immune response and growth factor. The amino acid sequence of Holstein cattle TLR6 shared high homology with that of yaks. Due to its role in the immune response and growth adjustment process, TLR6 gene may be responsible for disease resistance and production performance of Holstein cattle.

参考文献/References:

[1] TAKEDA K, KAISHO T, AKIRA S. Toll-like receptors[J].Annu Rev Immunol,2003,21:335-376.
[2] 吴 双,朱善元,王永娟,等. 鸡Toll 样受体2 的克隆表达及多克隆抗体的制备[J]. 江苏农业学报,2014,30(3):586-589.
[3] HIGGS R, CORMICAN P, CAHALANE S, et al. Induction of a novel chicken Toll-like receptor following salmonella enterica serovar typh in urium infection[J].Infect Immun,2006,74(3):1692-1698.
[4] 梁利国,陈 凯,谢 骏. Toll 样受体及其对水生动物疾病调控作用的研究进展[J]. 江苏农业科学,2015,43(5): 12-15.
[5] CAROLYN S J, NATHALIE A, JOSHUA M, et al. TLR signaling tailors innate immune responses in human microglia and astrocytes[J].The Journal of Immunology, 2005, 175(7): 4320-4330.
[6] VIKKI M A, PAULOMI B A, SHAUN P M, et al.TLR6 Modulates first trimester trophoblast responses to peptidoglycan[J]. The Journal of Immunology, 2008, 180(6): 6035-6043.
[7] MRODE R A, SWANSON G J T. Genetic and sta tistical properties of somatic cell count and its suitability as an indirect means of reducing the incidence of mastitis in dairy cattle[J].Animal Breeding Abstracts,1996,64(11):847-857.
[8] AXFORD R F E, BISHOP S C, NICHOLAS F W, et al.Breeding for disease resistance in farm animals [M].2nd Edition.Wallingford:CABI Publishers,2000:243-252.
[9] 林宝山,兰道亮,黄 偲,等.牦牛TLR6基因的克隆、序列分析及其表达分布研究[J].东北农业大学学报,2014,45(7):91-97.
[10] SHINKAL H, MUNETA Y, SUZUKI K, et al. Porcine Toll-like receptor 1,6,and 10 genes: complete sequencing of genomic region and expression analysis[J].Molecular Immunology,2006,43(9):1474-1480.
[11] OPSAL M A, VAGE D I, HAYES B, et al.Genomic organization and transcript profiling of the bovine Toll-like receptor gene cluster TLR6-TLR1-TLR10[J].Gene,2006,384:45-50.
[12] GURUPRASAD K, REDDY B V, PANDIT M W. Correlation between stability of a protein and its dipeptide composition:a novel approach for predicting in vivo stability of aprotein from its primary sequence[J]. Protein Engineering,1990,4(2):155 -161.
[13] NAKAI K, HORTON P. PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization[J]. Trends Biochem Sci,1999,24:34-36.
[14] ROST B, SANDER C. Combining evolutionary information and neural networks to predict protein secondary structure[J].Proteins,1994,19(1):55-72.
[15] PICCININI A M, MIDWOOD K S. DAM Pening inflammation by modulating TLR signaling[J]. Mediators Inflamm, 2010,2010(7):1-21.
[16] TANTISIRA K, KLIMECKI W T, LAZARUS R, et al. Toll-like receptor 6gene(TLR6): Single-nucleotide polymorphism frequencies andpreliminary association with the diagnosis of asthma[J]. Genesand Immunity, 2004, 5(456): 343-346.
[17] 魏 麟,陈 斌,黎晓英,等.猪TLR6基因MspⅠ多态性与生长性状相关性研究[J].中国农学通报, 2011, 27(11): 5-9.
[18] ISAMU S, HIROYUKI Y, CHUANYOU L, et al. Mycobacterial infectionin TLR2 and TLR6 knockout mice[J]. Microbiol Immunol, 2003,47(5): 327-336.
[19] 赵令斋,陈伟烈,胡凤玉,等. HIV/AIDS患者外周血白细胞TLR-1、-2、-4 和-6 表达的初步研究[J].中国病毒病杂志,2011, 1(5): 354-357.
[20] 韩猛立,黄 新,何延华,等.牛Toll样受体荧光定量PCR检测方法的建立[J].农业生物技术学报, 2011, 19(3): 521-529.
[21] 赵一萍,白东义,李 蓓,等.马Toll样受体表达水平SYBR Green I荧光定量RT-PCR检测方法的建立[J].畜牧兽医学报, 2013,44(2): 220-227.
[22] JUNG S C, GEORGE C R, OLIVER J, et al. Molecular cloning and characterization of Toll-like receptors 1-10 in sheep[J]. Veterinary Immunology and Immunopathology, 2009, 127(333): 94-105.
[23] IZARD J W, DOUGHTY M B, KENDALL D A. Physical and conformational properties of synthetic idealized signal sequences parallel their biologicalfunctior[J]. Biochemistry,1995,23(12): 9904-9906.
[24] 杨运桂,徐京宁,胡泰山.信号肽疏水性的提高促进青霉素G酰化酶分泌[J]. 生物化学与生物物理学报, 2000, 32(2): 163-168.
[25] LITYNSKA A, PRZYBYUULO M, POCHEC E, et al. Comparison of the lectin-binding pattern in different human melanoma cell lines[J].Melanoma Research,2001,11(3):205-212.
[26] 徐 飞,成述儒,罗玉柱.绵羊DRB1基因生物信息学分析[J].生物技术通报,2011(1):113-118.

备注/Memo

备注/Memo:
收稿日期:2015-12-21
基金项目:西北民族大学引进人才科研项目(xbmuyjrc201316); 西北民族大学国家级大学生创新创业训练计划项目(201510742084)
作者简介::李强子(1993-),男,河北赵县人,本科,主要从事分子生物学研究。(Tel)15751949497;(E-mail)lqz2015@sina.cn
通讯作者:张 丽,(Tel)13919959122;(E-mail)shiningstar2013@sina.cn
更新日期/Last Update: 2016-06-30