[1]付言峰,李 兰,李碧侠,等.梅山猪胚胎着床期EphB6基因的mRNA和蛋白表达[J].江苏农业学报,2016,(03):575.[doi:10.3969/j.issn.1000-4440.2016.03.014]
　FU Yan-feng,LI Lan,LI Bi-xia,et al.The mRNA and protein expression patterns of EphB6 during embryo implantation in Meishan sows[J].,2016,(03):575.[doi:10.3969/j.issn.1000-4440.2016.03.014]
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梅山猪胚胎着床期EphB6基因的mRNA和蛋白表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
575
栏目:
畜牧兽医·水产养殖
出版日期:
2016-06-30

文章信息/Info

Title:
The mRNA and protein expression patterns of EphB6 during embryo implantation in Meishan sows
作者:
付言峰1; 2;  李 兰3; 4;  李碧侠1;  方晓敏1;  王学敏1;  赵为民1;  任守文1
1.江苏省农业科学院畜牧研究所/动物品种改良和繁育重点实验室/江苏省农业种质资源保护与利用平台,江苏 南京 210014; 2.伊利诺伊大学厄巴纳-香槟分校动物科学实验室,伊利诺伊 厄巴纳 61801; 3.中国农业大学动物医学院,北京 100193; 4.国家兽用生物制品工程技术研究中心,江苏 南京 210014
Author(s):
FU Yan-feng1; 2;  LI Lan3; 4;  LI Bi-xia1;  FANG Xiao-min1;  WANG Xue-min1;  ZHAO Wei-min1; REN Shou-wen1
1.Institute of Animal Science/The Key Laboratory of Animal Breeding Improvement and Reproduction, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, China; 2. Animal Science Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801,USA; 3.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; 4. National Research Center of Veterinary Biologicals Engineering and Technology,Nanjing 210014, China
关键词:
EphB6基因;  表达;  梅山猪
Keywords:
EphB6;  embryo implantation;  expression;  Meishan swine
分类号:
S828.8+1
DOI:
10.3969/j.issn.1000-4440.2016.03.014
文献标志码:
A
摘要:
为了研究促红细胞生成素产生肝细胞受体B6(EphB6)在猪胚胎着床期子宫内膜上皮细胞和胚胎之间的迁移和粘附活动中是否发挥了作用,本研究利用实时荧光定量PCR方法和蛋白免疫印迹方法,分析了EphB6基因在太湖流域梅山猪胚胎着床前期(妊娠第13 d)、中期(妊娠第18 d)和后期(妊娠第24 d)子宫内膜和卵巢组织的mRNA和蛋白表达。结果表明, EphB6在胚胎着床期猪子宫内膜着床点和着床点间的mRNA和蛋白均呈现先升高后降低的表达趋势,即着床中期(妊娠第18 d)的表达量极显著高于前期(妊娠第13 d)和后期(妊娠第24 d)(P<0.01); EphB6在胚胎中的mRNA表达也是妊娠第18 d极显著高于妊娠第24 d(P<0.01); 而EphB6在卵巢中的mRNA和蛋白表达相反:mRNA表达为先降低后升高,蛋白表达为先升高后降低。说明,EphB6基因很可能在猪胚胎着床过程中发挥着重要的调控作用,可成为潜在的猪产仔数性状候选基因。
Abstract:
To define the role of erythropoietin-producing hepatocellular receptor B6(EphB6)in the migration and attachment between embryo and endometrium during porcine embryo implantation, the mRNA and protein expressions of EphB6 in endometrium and ovary were detected in Meishan sows during pre-implantation(pregnancy 13 d, 13 d), mid-implantation(18 d)and post-implantation(24 d)using real-time quantitative PCR and Western blot. Both EphB6 mRNA and protein in endomtrium showed the same expression pattern, an increase from pre-implantation to mid-implantation and a decrease from mid-implantation to post-implantation. In embryo, mRNA expression on day 18 was significantly higher than that on day 24(P<0.01). In ovary, EphB6 mRNA expression level dropped and then picked up, which was quite the contrary for protein expression level. In conclusion, EphB6 may play an important role in swine embryo implantation, and is a candidate gene for litter size in swine.

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备注/Memo

备注/Memo:
收稿日期:2015-10-17
基金项目:国家自然科学基金项目(31201767); 江苏省农业科学院第5批留学基金项目; 国家生猪产业技术体系南京综合试验站项目(CARS-36); 江苏省农业科技自主创新基金项目[CX(13)2037]
作者简介::付言峰(1982-),男,山东聊城人,博士, 副研究员, 主要从事猪遗传育种与繁殖研究。(E-mail)yanfeng8@illinois.edu。李兰为共同第一作者。
通讯作者:任守文, 025-84390349;(E-mail)shouwenren@163.com
更新日期/Last Update: 2016-06-30