[1]周建涛,姜雪婷,李 慧,等.钙调素及其抗血清对梨花粉萌发和花粉管生长的影响[J].江苏农业学报,2016,(02):437-441.[doi:10.3969/j.issn.1000-4440.2016.02.030]
　ZHOU Jian-tao,JIANG Xue-ting,LI Hui,et al.Germination and growth of pear pollen in response to calmodulin and its antiserum[J].,2016,(02):437-441.[doi:10.3969/j.issn.1000-4440.2016.02.030]
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钙调素及其抗血清对梨花粉萌发和花粉管生长的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
437-441
栏目:
园艺
出版日期:
2016-03-20

文章信息/Info

Title:
Germination and growth of pear pollen in response to calmodulin and its antiserum
作者:
周建涛1; 2;  姜雪婷2;  李 慧1;  蔺 经1;  张绍铃2
1.江苏省农业科学院园艺研究所/江苏省高效园艺作物遗传改良重点实验室,江苏 南京 210014; 2南京农业大学梨工程技术研究中心,江苏 南京 210095
Author(s):
ZHOU Jian-tao1; 2;  JIANG Xue-ting2;  LI Hui1;  LIN Jing1;  ZHANG Shao-ling2
1.Institute of Horticulture, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China; 2.Pear Engineering Research Centre, Nanjing 210095, China
关键词:
钙调素;  梨;  自交不亲和;  花粉特性
Keywords:
calmodulin;  pear;  selfing incompatibility;  pollen characteristic
分类号:
S661.2
DOI:
10.3969/j.issn.1000-4440.2016.02.030
文献标志码:
A
摘要:
以砂梨品种今村秋及丰水为试验材料,在离体及活体条件下,研究了外源施加不同浓度钙调素及其抗血清对梨花粉萌发及花粉管生长的影响。离体培养条件下,施加10-7 mol/L 钙调素对今村秋花粉萌发及花粉管生长的促进作用最明显,而稀释200倍的钙调素抗血清则有明显的抑制作用。自交不亲和性反应与活体试验结果进一步证实钙调素在梨花粉萌发与花粉管生长过程的促进作用,而钙调素抗血清的作用与之截然相反,显著抑制花粉萌发及花粉管生长。说明,钙调素处理可以有效促进梨自交不亲和过程花粉萌发及花粉管生长。
Abstract:
The influences of different concentrations of exogenous calmolulin and its antiserum on the pollen germination and growth of pear were studied on Pyrus pyrifolia varieties Imamuraaki and Hosui in vitro and in vivo. The application of 10-7 mol/L calmodulin could promote pollen germination and pollen tube growth in vitro, however, 200-times diluted calmodulin antiserum showed inhibitory effect. The functions of calmodulin and its antiserum in pollen germination and pollen tube growth were further proved by selfing incompatibility reaction and in vivo experiment. The results suggest that calmodulin treatment can effectively boost pollen germination and pollen tube growth of pear during selfing incompatibility process.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-08-24 基金项目:国家现代农业(梨)产业技术体系项目(CARS-29) 作者简介:周建涛(1965-),男,博士,研究员,主要从事果树种质资源研究。(E-mail)102949231@qq.com 通讯作者:张绍铃,(E-mail)nnzsl@njau.edu.cn
更新日期/Last Update: 2016-03-20