[1]蔡之军,周德银,高荣村,等.水稻抗褐飞虱基因Bph14和Bph15在粳稻育种上的应用[J].江苏农业学报,2016,(02):257-261.[doi:10.3969/j.issn.1000-4440.2016.02.003]
　CAI Zhi-jun,ZHOU De-yin,GAO Rong-cun,et al.Application of brown planthopper-resistant genes Bph14 and Bph15 in Japonica rice breeding[J].,2016,(02):257-261.[doi:10.3969/j.issn.1000-4440.2016.02.003]
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水稻抗褐飞虱基因Bph14和Bph15在粳稻育种上的应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
257-261
栏目:
遗传育种·生理生化
出版日期:
2016-03-20

文章信息/Info

Title:
Application of brown planthopper-resistant genes Bph14 and Bph15 in Japonica rice breeding
作者:
蔡之军1;  周德银2;  高荣村1;  王建华3 ;  李金军1
1.浙江省嘉兴市农业科学研究院,浙江 嘉兴 314016; 2.江苏省兴化市农业委员会,江苏 兴化 225700; 3.江苏省里下河地区农业科学研究所,江苏 扬州 225007
Author(s):
CAI Zhi-jun1;  ZHOU De-yin2;  GAO Rong-cun 1;  WANG Jian-hua3;  LI Jin-jun1
1.Jiaxing Academy of Agricultural Sciences, Zhejiang Province Jiaxing 314016, China; 2.Xinghua Agricultural Commission, Xinghua 225700, China; 3.Institute of Agricultural Sciences of the Lixiahe Districtin Jiangsu Province, Yangzhou 225007, China
关键词:
粳稻;  褐飞虱;  导入系;  分子标记辅助选择
Keywords:
Japonica rice;  brown planthopper;  introgression line;  marker-assisted selection(MAS)
分类号:
S511.034
DOI:
10.3969/j.issn.1000-4440.2016.02.003
文献标志码:
A
摘要:
以B5为褐飞虱抗性供体亲本,以粳稻品种(品系)嘉隆17B和嘉58为受体亲本,通过杂交、回交和标记选择,获得Bph14-嘉隆17B、Bph15-嘉隆17B、Bph14/Bph15 -嘉隆17B及Bph14 -嘉58、Bph15-嘉58、Bph14/Bph15-嘉58导入系。苗期和抽穗期抗性鉴定结果显示,在苗期Bph14/Bph15-嘉隆17B或Bph14/Bph15-嘉58的抗性级别为1.0级(高抗),Bph14 -嘉隆17B或Bph14 -嘉58、Bph15 -嘉隆17B或Bph15 -嘉58抗性为 3.0～7.0级(抗、中抗、中感),双基因导入系抗性显著高于单基因导入系; 同时发现Bph14-嘉隆17B抗性水平(4.56级)低于Bph14-嘉58(2.50级),Bph15-嘉隆17B抗性水平(3.80级)高于Bph15-嘉58(5.86级); 抽穗期的抗性水平与苗期表现一致或接近。说明Bph14 、Bph15在粳稻背景中能够表达,但表达水平因背景不同而有差异,水稻苗期的抗性可作为抗性品种选育的参考依据,利用分子标记技术辅助选育抗褐飞虱粳稻品种是切实可行的。
Abstract:
A rice line B5, with Bph14 and Bph15 genes controlling the resistance to brown planthopper(BPH), was crossed with Jialong17B and Jia58, two Japonica varieties developed in Zhejiang province. The introgression lines, Bph14-Jia58, Bph15-Jia58, Bph14/Bph15-Jia58 and Bph14-Xiushui134, Bph15-Xiushui134, Bph14/Bph15-Xiushui134, were obtained via crossing, backcrossing and marker-assisted selection. The resistances to BPH were higher in double-gene introgression lines Bph14/Bph15- Jialong17B and Bph14/Bph15- Jia58(highly resistant)than those in single gene introgression lines(resistant, moderately resistant and moderately susceptible). Line Bph14-Jia58 was more resistant than line Bph14-Jialong17B, but line Bph15-Jialong17B was more resistant than line Bph15-Jia58 at seedling stage. At heading stage, the resistance levels of most introgression lines were consistent with those at seedling stage. This study indicates that Bph14 and Bph15 genes from B5 could be expressed in Japonica, and expression levels varied with the background. The selection of BPH-resistance could be conducted at seedling stage for marker-assisted selection of BPH-resistant Japonica.

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备注/Memo

备注/Memo:
收稿日期:2015-12-03 基金项目:浙江省嘉兴市重点项目(2012AZ1004); 浙江省重大项目(2010C1202) 作者简介:蔡之军(1978-),男,安徽泗县人,硕士,农艺师,主要从事杂交粳稻育种研究。(Tel)13067625579;(E-mail)czj982002@126.com 通讯作者:李金军,(E-mail)lijinjunjx@163.com
更新日期/Last Update: 2016-03-20