[1]强新涛,赵春芳,赵 凌,等.籼稻栽培品种中淀粉合成相关基因的遗传变异和群体结构分析[J].江苏农业学报,2016,(02):241-249.[doi:10.3969/j.issn.1000-4440.2016.02.001]
　QIANG Xin-tao,ZHAO Chun-fang,ZHAO Ling,et al.Analysis of genetic variation and population structure of starch synthesis related genes in indica rice cultivars[J].,2016,(02):241-249.[doi:10.3969/j.issn.1000-4440.2016.02.001]
点击复制

籼稻栽培品种中淀粉合成相关基因的遗传变异和群体结构分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
241-249
栏目:
遗传育种·生理生化
出版日期:
2016-03-20

文章信息/Info

Title:
Analysis of genetic variation and population structure of starch synthesis related genes in indica rice cultivars
作者:
强新涛1; 2;  赵春芳2;  赵 凌2;  赵庆勇2;  陈 涛2;  周丽慧2;  姚 姝2;  王才林1; 2
1.南京农业大学农学院/江苏省现代作物生产协同创新中心,江苏 南京 210095; 2.江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,江苏 南京 210014
Author(s):
QIANG Xin-tao1; 2;  ZHAO Chun-fang2;  ZHAO Ling2;  ZHAO Qing-yong2;  CHEN Tao2;  ZHOU Li-hui2;  YAO Shu2;  WANG Cai-lin1; 2
1.College of Agriculture, Nanjing Agricultural University / Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210095, China; 2.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Jiangsu High Quality Rice R&D Center / Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China
关键词:
籼稻;  淀粉合成相关基因;  遗传变异;  群体结构
Keywords:
indica rice;  starch synthesis related gene;  genetic variation;  population structure
分类号:
S511.2+1
DOI:
10.3969/j.issn.1000-4440.2016.02.001
文献标志码:
A
摘要:
选用34个淀粉合成相关基因分子标记对87份来自不同国家的籼稻栽培品种进行遗传变异和群体结构分析。结果表明,34对引物共检测到80个等位变异,平均每个位点含2.35个等位基因,品种间等位基因变异范围 2～6; Shannon's信息指数变幅为 0.303～0.796,平均为0.539; 多态信息含量(PIC)的变幅为 0.084～0.658,平均0.295; 遗传相似系数变幅为0.265～0.990,表明淀粉合成相关基因在品种间存在遗传差异,但不同品种间等位基因的变异频率不同。87份籼稻品种可以分成3个类群,类群内品种遗传背景比较相似,类群间品种遗传背景差异明显,其中,39.1%的籼稻品种遗传组分单一,而遗传背景复杂的籼稻品种达到60.9%。本研究可为水稻淀粉品质的遗传改良提供依据,为后续淀粉品质性状的关联分析奠定基础。
Abstract:
34 molecular markers of starch synthesis related genes were used to evaluate the genetic variation and population structure of 87 indica rice cultivars from different countries. A total of 80 alleles were amplified using 34 primer pairs, with an average of 2.5 alleles per primer pair. Shannon index ranged from 0.303 to 0.796, with an average of 0.539. The polymorphism information content(PIC)value ranged from 0.084 to 0.658, with an average of 0.295. The genetic similarity ranged from 0.265 to 0.990, indicating the significant genetic differences of starch synthesis related genes in the varieties, even though the variation frequency of alleles varied in different varieties. Population structure analysis showed that 87 indica rice cultivars were classified into 3 groups. The genetic differences were small within an individual group but great among the groups. This result will provide not only theoretical basis for genetic improvement of rice starch quality, but also molecular information for association analysis of rice quality-related traits in the future.

参考文献/References:

[1] 何秀英,廖耀平,程永盛,等.水稻品质研究进展与展望[J].广东农业科学,2009(1):11-16.
[2] 包劲松.稻米淀粉品质遗传与改良研究进展[J].分子植物育种,2007,6(S):1-20.
[3] NAKAMURA Y. Towards a better understanding of the metabolic system for amylopectin biosynthesis in plants: rice endosperm a model tissue[J]. Plant Cell Physiology, 2002, 43(7): 718-725.
[4] JAMES M G, DENYER K, MYERS A M, et al. Starch synthesis in the cerealendosperm[J]. Curr Opin Plant Biol, 2003,56(12):215-222.
[5] HANNAH L C, JAMES M. The complexities of starch biosynthesis in cereal endosperms[J]. Curr Opin Biotechnol, 2008, 19(24): 160-165.
[6] TIAN Z X, QIAN Q, LIU Q Q, et al. Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities[J]. PNAS, 2009, 106(51): 21760-21765.
[7] 吴洪凯,梁国华,严长杰,等.水稻不同生态型品种间直链淀粉含量的变异及其遗传分析[J].作物学报,2006,32(9):1301-1305.
[8] 康翠芳,向珣朝,龙小林,等.水稻淀粉合成相关基因SSI、SSIII-1和PUL对稻米品质的影响[J].农业生物技术学报,2015,23(3):311-319.
[9] 刘燕清,强新涛,赵春芳,等. 水稻淀粉合成相关基因分子标记的筛选与利用[J]. 江苏农业学报,2015,31(3):471-476.
[10] 陈家宽,杨 继.植物进化生物学[M].武汉:武汉大学出版社,1994:15-208.
[11] 刘志斋,吴 迅,刘海利,等.基于40个核心SSR标记揭示的820份中国玉米重要自交系的遗传多样性和群体结构分析[J].中国农业科学,2012,45(11):2107-2138.
[12] 刘 杨,黄 海,陶建蜀,等.用改良CTAB法从吴茱萸不同组织提取基因组DNA[J].贵阳医学院学报,2009,34(1):101-103.
[13] 赵 翀,刘法央,郭玉霞,等.AFLP荧光标记和银染技术的比较分析[J].甘肃农业大学学报,2007,4(2):125-129.
[14] YEH F C, YANG R C, BOYLE T B J, et al. POPGENE, the userfriendly shareware for population genetic analysis [M]. Canada: Molecular Biology and Biotechnology Centre, University of Alberta,1999.
[15] ANDERSON J A, CHURCHILL G A, AUTRIQUE J E, et al. Optimizing parental selection for genetic linkage maps[J]. Genome, 1993,36(1):181-186.
[16] ROHLF F J. Numerical taxonomy and multivariate analysis system NTSYS-PC version 2.1 user guide [M]. New York:Exeter Software Setauket, 2000.
[17] PRITCHARD J, STEPHENS M, DONNELLY P. Inference of population structure using multilocus genotype data[J]. Genetics,2000,155: 945-959.
[18] EVANNO G, REGNAUT S, GOUDET J. Dtetcting the number of clusters of individuals using the software STRUCTURE:a simulation study[J].Molecular Ecology,2005,14(8):2611-2620.
[19] WANG R, YU Y, ZHAO J, et al. Population structure and linkage disequilibrium of a mini core set of maize in bred lines in China[J].Theoritical and Applied Genetics,2008,117(7):1141-1153.
[20] 刘丽华,王丽新,赵昌平,等.光温敏二系杂交小麦恢复系遗传多样性和群体结构分析[J].中国生物化学与分子生物学报,2009,25(9):867-875.
[21] 陈 斐,魏臻武,李伟民,等.基于SSR标记的苜蓿种质资源遗传多样性与群体结构分析[J].草地学报,2013,21(4):759-768.
[22] 陈氏秋江,党小景,刘强明,等.水稻籽粒性状的SSR关联分析[J].中国水稻科学,2014,28(3):243-257.
[23] 孙晓棠,卢东东,欧阳林娟,等.水稻纹枯病抗性关联分析及抗性等位变异发掘[J].作物学报,2014,40(5):779-787.

备注/Memo

备注/Memo:
收稿日期:2015-11-09 基金项目:江苏省农业科技自主创新基金项目[CX(14)5107]; 江苏省科技支撑计划项目(BE2015335); 现代农业产业技术体系建设专项(CARS-01-47) 作者简介:强新涛(1989-),男,河南信阳人,硕士研究生,主要从事水稻遗传育种研究。(Tel)15150531273;(E-mail)qiangxintao2008@126.com
更新日期/Last Update: 2016-03-20