[1]李碧侠,赵芳,任守文,等.SIRT1 基因对猪卵巢颗粒细胞中生殖激素受体基因表达量的影响[J].江苏农业学报,2016,(01):123-127.[doi:10.3969/j.issn.1000-4440.2016.01.019 ]
 LI Bi-xia,ZHAO Fang,REN Shou-wen,et al.Role of SIRT1 gene in expression of reproductive hormone receptor genes in porcine ovarian granulosa cells[J].,2016,(01):123-127.[doi:10.3969/j.issn.1000-4440.2016.01.019 ]
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SIRT1 基因对猪卵巢颗粒细胞中生殖激素受体基因表达量的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年01期
页码:
123-127
栏目:
畜牧兽医·水产养殖
出版日期:
2016-01-08

文章信息/Info

Title:
Role of SIRT1 gene in expression of reproductive hormone receptor genes in porcine ovarian granulosa cells
作者:
李碧侠赵芳任守文付言峰
(江苏省农业科学院畜牧研究所/江苏省农业种质资源保护与利用平台,江苏南京210014)
Author(s):
LI Bi-xiaZHAO FangREN Shou-wenFU Yan-feng
(Institute of Animal Husbandry, Jiangsu Academy of Agricultural Sciences/ Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing210014, China)
关键词:
卵巢颗粒细胞SIRT1生殖激素受体
Keywords:
porcineovarian granulosa cellSIRT1reproductive hormone receptor
分类号:
S634.3
DOI:
10.3969/j.issn.1000-4440.2016.01.019
文献标志码:
A
摘要:
为了探讨SIRT1基因在猪卵巢颗粒细胞生殖激素分泌中的作用,采用白藜芦醇和尼克酰胺处理猪卵巢颗粒细胞,检测猪卵巢颗粒细胞中SIRT1基因和生殖激素受体表达水平,分析SIRT1基因与生殖激素受体表达量的相关性。结果表明,白藜芦醇和尼克酰胺可剂量依赖性地调节猪卵巢颗粒细胞中SIRT1基因表达,SIRT1基因表达量的升高可引起ER2、FSHR、LHR表达量上调,其相关系数分别为 0.820 6、0.519 7和 0.140 7。说明,SIRT1基因参与猪卵巢颗粒细胞生殖激素受体表达调控,可影响猪卵巢颗粒细胞生殖激素分泌。
Abstract:
To explore the role of SIRT1 gene in secretion of reproductive hormones in porcine ovarian granulosa cells,quantitative real-time PCR was employed to detect the mRNA expressions of SIRT1 gene and reproductive hormone receptors genes in granulosa cells treated with resveratrol and nicotinamide. The correlation between expression levels of SIRT1 and reproductive hormone receptors genes was also analysised by SPSS software. Results showed that expression of SIRT1 gene was regulated by resveratrol and nicotinamide in granulosa cells in a dose-dependent manner. With the upregulation of SIRT1 mRNA expression, mRNA expressions of ER2, FSHR, LHR were increased. Correlations between SIRT1 and ER2expressions, between SIRT1 and FSHR expressions and between SIRT1 and LHR expressions were positively linear, with the correlation coefficients of 0.820 6, 0.519 7 and 0.140 7, respectively. These results suggest that SIRT1 may take part in the regulation of reproductive hormone receptors expressions, thereby affecting the secretion of reproductive hormones in porcine ovarian granulosa cells.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-07-06 基金项目:国家生猪现代产业技术体系南京综合试验站项目(CARS-36);江苏省农业种质资源库项目(BM2011134); 江苏省农业科技自主创新基金项目[CX(14)2069] 作者简介:李碧侠(1979-),女,江苏泗阳人,博士,副研究员,主要从事动物遗传育种与繁殖研究。(E-mail)bxli790@ sina.com
更新日期/Last Update: 2017-05-08