[1]应诗家,戴子淳,郭佳佳,等.鹅输卵管TLR家族基因差异表达的研究[J].江苏农业学报,2015,(06):1395-1399.[doi:doi:10.3969/j.issn.1000-4440.2015.06.031]
　YING Shi-jia,DAI Zi-chun,GUO Jia-jia,et al.Differential gene expressions of Toll-like receptors in goose oviduct[J].,2015,(06):1395-1399.[doi:doi:10.3969/j.issn.1000-4440.2015.06.031]
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鹅输卵管TLR家族基因差异表达的研究()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1395-1399
栏目:
畜牧兽医·水产养殖
出版日期:
2015-12-31

文章信息/Info

Title:
Differential gene expressions of Toll-like receptors in goose oviduct
作者:
应诗家1; 戴子淳12; 郭佳佳1; 李辉1; 雷明明1; 施振旦1; 沈明君3
（1.江苏省农业科学院畜牧研究所/动物品种改良和繁育重点实验室，江苏南京210014；2.南京农业大学动物科技学院，江苏南京210095；3.扬州朝天歌农牧科技有限公司，江苏扬州225000）
Author(s):
YING Shi-jia1; DAI Zi-chun12; GUO Jia-jia1; LI Hui1; LEI Ming-ming1; SHI Zhen-dan1
(1.Institute of Animal Science/Laboratory of Animal Improvement and Reproduction, Jiangsu Academy of Agricultural Science, Nanjing 210014, China;2.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;3.Yangzhou Goosing Agricultural Science and Technology Co.,Ltd., Yangzhou 225000, China)
关键词:
鹅; 输卵管; Toll样受体; 基因表达
Keywords:
goose; oviduct; Toll-like receptor (TLR); gene expression
分类号:
S835
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.031
文献标志码:
A
摘要:
本试验旨在研究已公开的10种鸟类Toll样受体（TLRs）家族基因在鹅输卵管不同功能部位的差异表达。分别采用RT-PCR和qRT-PCR技术检测输卵管基因表达谱和表达水平。结果显示除TLR1-1和TLR5分别在膨大部和子宫部组织无表达外，其他TLR家族基因在输卵管不同部位均表达，其中4个基因在阴道部高表达，2个基因分别在伞部和峡部高表达，1个基因分别在膨大部和子宫部高表达；TLR2-2和TLR21在输卵管不同功能部位间表达差异不显著 （P>0.05）；阴道部TLR1-1表达显著高于伞部 （P<0.05），TLR3表达显著高于伞部、峡部和膨大部 （P<0.05），TLR5表达显著高于伞部和膨大部 （P<0.05），TLR15表达显著高于子宫部 （P<0.05）；伞部TLR1-2和TLR2-1表达显著高于峡部和阴道部 （P<0.05）；峡部TLR4表达显著高于子宫部和阴道部 （P<0.05），TLR5显著高于伞部和膨大部 （P<0.05）；膨大部TLR2-1表达显著高于峡部和阴道部 （P<0.05）；子宫部TLR7表达显著高于伞部、峡部、膨大部和阴道部 （P<0.05）。表明鹅输卵管不同功能部位差异化表达TLR，存在抵御病原微生物侵染的非特异性免疫反应机制。
Abstract:
This study aims to investigate the gene expression of Toll-like receptors (TLRs) in different segments of goose oviduct. The profiles and levels of TLRs gene expressions in all segments of goose oviduct were examined by RT-PCR and qRT-PCR, respectively. Except for non-detectable 〖WTBX〗TLR1-1 and 〖WTBX〗TLR5 expressions in magnum and uterus, respectively, the others were all expressed in oviduct. Among 10 TLRs genes, 4 were highly expressed in vagina tissue, 2 in infundibulum and isthmus tissues, respectively, and 1 in magnum and uterus tissues, respectively. Gene expressions of 〖WTBX〗TLR2-2 and 〖WTBX〗TLR21 differed insignificantly in various segments of oviduct （P>0.05）. Compared with gene expression in vagina, 〖WTBX〗TLR1-1 mRNA expression in infundibulum, 〖WTBX〗TLR3 mRNA expressions in infundibulum, isthmus and magnum, 〖WTBX〗TLR5 mRNA expressions in infundibulum and magnum, and 〖WTBX〗TLR15 mRNA expression in uterus were significantly decreased （P<0.05）. Compared with gene expression in infundibulum, 〖WTBX〗TLR1-2 and 〖WTBX〗TLR2-1 mRNA expressions in isthmus and vagina were significantly decreased （P<0.05）. 〖WTBX〗TLR4 and 〖WTBX〗TLR5 mRNA expressions in isthmus were higher than those in uterus and vagina and those in infundibulum and magnum, respectively. 〖WTBX〗TLR2-1 mRNA expression in magnum was higher than those in isthmus and vagina, and 〖WTBX〗TLR7 mRNA expression in uterus was higher than those in the other tissues. In conclusion, differential expression of Toll-like receptors genes may play essential roles in oviductal innate immunity for host defense

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备注/Memo

备注/Memo:
收稿日期：2015-07-10 基金项目：江苏省农业科技自主创新基金项目[CX(13)5041]；江苏省自然科学基金项目（BK20130718）；国家水禽产业技术体系（CARS-43-16） 作者简介：应诗家（1984-），男，安徽巢湖人，博士，助理研究员，研究方向为家禽环境控制。（Tel）025-84390772；（E-mail）ysj@jaas.ac.cn 通讯作者：施振旦，（Tel）025-84390956;（E-mail）zdshi@jaas.ac.cn
更新日期/Last Update: 2015-12-31