[1]齐中强,薛延丰,张猛,等.蛇床子素对稻瘟病菌侵染的影响[J].江苏农业学报,2015,(06):1265-1269.[doi:doi:10.3969/j.issn.1000-4440.2015.06.011]
　QI Zhong-qiang,XUE Yan-feng,ZHANG Meng,et al.Effect of osthol on the invasion of Magnaporthe oryzae[J].,2015,(06):1265-1269.[doi:doi:10.3969/j.issn.1000-4440.2015.06.011]
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蛇床子素对稻瘟病菌侵染的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1265-1269
栏目:
植物保护
出版日期:
2015-12-31

文章信息/Info

Title:
Effect of osthol on the invasion of Magnaporthe oryzae
作者:
齐中强12; 薛延丰1; 张猛1; 李丽娜1; 石志琦1
（1.江苏省农业科学院食品质量安全与检测研究所，江苏南京210014；2.江苏省农业科学院植物保护研究所，江苏南京210014）
Author(s):
QI Zhong-qiang12; XUE Yan-feng1; ZHANG Meng1; LI Li-na1; SHI Zhi-qi1
(1.Institute of Food Safety,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
关键词:
蛇床子素; 稻瘟病菌; 附着胞; 细胞壁完整性
Keywords:
osthol; Magnaporthe oryzae; appressorium; cell wall integrity
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.011
文献标志码:
A
摘要:
为了探究蛇床子素对稻瘟病菌致病力的影响，采用生长速率和致病力测定的方法对其进行研究。室内生长速率测定结果表明，蛇床子素对稻瘟病菌的生长有抑制作用，32 μg/ml浓度下，菌丝生长开始受到抑制，160 μg/ml浓度下，抑制率达到65%左右；且液体培养状态下，抑制作用更加明显。扫描电镜观察发现，60 μg/ml蛇床子素处理条件下，菌丝呈现畸形，菌丝表面出现干瘪及皱缩，部分菌丝出现断裂等现象。致病性试验结果表明，20 μg/ml蛇床子素可降低稻瘟病菌对水稻的致病力，40 μg/ml蛇床子素可完全抑制稻瘟病菌的侵染；同时附着胞形成试验发现，20 μg/ml蛇床子素能够部分抑制附着胞的形成，40 μg/ml蛇床子素则能完全抑制孢子的萌发。
Abstract:
The influence of osthol on the pathogenicity of Magnaporthe oryza was studied by detecting the growth rate and morphology of mycelia. The colony growth was inhibited when treated with 32 μg/ml osthol and the inhibition rate was about 65% when treated with 160 μg/ml osthol. The scanning electron microscope revealed clear morphological alteration (abnormality,wizening and fracture) of mycelia of M.oryzae with 60 μg/ml osthol treatment.The pathogenicity was reduced by the application of 20 μg/ml osthol,and was totally lost with 40 μg/ml osthol.Appressorium formation was partially inhibited with 20 μg/ml osthol and the conidia could not germinate at all with 40 μg/ml osthol.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2015-08-04 基金项目：江苏省青年基金项目（BK20140749） 作者简介：齐中强（1986-），男，河南驻马店人，博士，助理研究员，主要从事稻瘟病菌功能基因研究。(Tel)025-84391810；(E-mail)2006@126.com 通讯作者：石志琦,(Tel)025-84391863；(E-mail)shizhiqi@jaas.ac.cn
更新日期/Last Update: 2015-12-31