[1]吴旭江,臧淑江,程凯,等.小麦重组自交系群体抽穗期QTL分析[J].江苏农业学报,2015,(06):1199-1205.[doi:doi:10.3969/j.issn.1000-4440.2015.06.002]
　WU Xu-jiang,ZANG Shu-jiang,CHENG Kai,et al.QTL mapping of heading date of wheat recombinant inbred line population[J].,2015,(06):1199-1205.[doi:doi:10.3969/j.issn.1000-4440.2015.06.002]
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小麦重组自交系群体抽穗期QTL分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1199-1205
栏目:
遗传育种·生理生化
出版日期:
2015-12-31

文章信息/Info

Title:
QTL mapping of heading date of wheat recombinant inbred line population
作者:
吴旭江12; 臧淑江1; 程凯1; 张伯桥1; 张勇1
(1.江苏里下河地区农业科学研究所／农业部长江中下游小麦生物学与遗传育种重点实验室,江苏扬州225007;2.扬州大学粮食作物现代产业技术协同创新中心,江苏扬州225009)
Author(s):
WU Xu-jiang12; ZANG Shu-jiang1; CHENG Kai1; ZHANG Bo-qiao1; ZHANG Yong1
(1. Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province／Key Laboratory of Wheat Biology and Genetic Breeding in the Middle and Lower Yangtze River, Ministry of Agriculture,Yangzhou 225007,China;2.Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,China)
Keywords:
wheat(Triticum aestivum L.); heading date; QTL
分类号:
S512.103.2
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.002
文献标志码:
A
摘要:
为了对小麦抽穗期进行QTL初步定位,并进行遗传分析,以CI12633×扬麦9号重组自交系(RIL)为材料,在3年9次试验中,记载小麦抽穗期,采用复合区间作图法进行小麦抽穗期QTL分析。共检测到7个QTL,分别位于1A、4D、5B、6B和7A 5条染色体上,贡献率为5.76％～15.26％。其中位于4D染色体上分子标记Xgpw5191至Xmag1163之间的Qhd-4D基因在3年9次试验中都被检测到,是一个稳定表达的与抽穗期相关的基因。同时，不同环境条件下检测到不同的抽穗期QTL,说明环境对小麦抽穗期影响较大。
Abstract:
A recombinant inbred line (RIL) population derived from a cross between two common wheat cultivars CI12633／Yangmai9＃ was used for QTL mapping of heading date. Data was collected from 9 repeated trials in three years. Seven QTLs located on chromosomes 1A,4D,5B,6B and 7A were detected, contributing 5.76％ to 15.26％ to the total phenotypic variation by composite interval mapping(CIM). The gene Qhd-4D on 4D, between marker Xgpw5191 and Xmag1163, detected in all nine trials, was a stable expression gene related to heading date. Various QTLs were detected in different environments, suggesting that heading date is sensitive to environment.

参考文献/References:

[1]SNAPE J W,BUTTERWORTH K,WHITECHURCH E,et al. Waiting for fine times: genetics of flowering time in wheat[J]. Euphytica,2001,119(1-2):185-190.
[2]BULLRICH L,APPENDINO M,TRANQUILLI G,et al. Mapping of a thermo-sensitive earliness per se gene on Triticum monococcum chromosome 1Am[J]. Theoretical and Applied Genetics,2002,105(4):585-593.
[3]LAW C N,SUTKA J,WORLAND A J. A Genetic study of day-length response in wheat[J]. Heredity,1978,41:185-191.
[4]SCARTH R,LAW C N. The control of the day-length response in wheat by the group 2 chromosomes[J]. Heredity,1984,92(2):140-150.
[5]SCARTH R,LAW C N. A genetic study of daylength in wheat by the group 2 chromosome 2B of wheat[J]. Heredity,1983,51:607-619.
[6]WORLAND A J,BRNER A,KORZUN V,et al. The influence of photoperiod genes on the adaptability of European winter wheats[J]. Euphytica,1994,100(1-3):385-394.
[7]DANYLUK J,KANE N A,BRETON G,et al. TaVRT-1,a putative transcription factor associated with vegetative to reproductive transition in cereals[J]. Plant Physiology,2003,132(4):1849-1860.
[8]TREVASKIS B,BAGNALL D J,ELLIS M H,et al. MADS box genes control vernalization-induced flowering in cereals[J]. Proceedings of the National Academy of Sciences of the United States of America,2003,100(22):13099-13104.
[9]YAN L,LOUKOIANOV A,TRANQUILLI G,et al. Positional cloning of the wheat vernalization gene VRN1[J]. Proceedings of the National Academy of Sciences of the United States of America,2003,100(10):6263-6268.
[10]LIULING Y,ARTEM L,ANN B,et al. The wheat VRN2 gene is a flowering repressor down-regulated by vernalization[J]. Science,2004,303(5664):1640-1644.
[11]YAN L,FU D,LI C,et al. The wheat and barley vernalization gene VRN3 is an orthologue of FT[J]. Proceedings of the National Academy of Sciences of the United States of America,2006,103(51):19581-19586.
[12]YOSHIDA T,NISHIDA H,ZHU J,et al. Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat[J]. TAG Theoretical and Applied Genetics Theoretische and Angewandte Genetik,2010,120(3):543-552.
[13]YAN L,HELGUERA M,KATO K,et al. Allelic variation at the VRN-1 promoter region in polyploid wheat[J]. Theoretical and Applied Genetics,2004,109(8):1677-1686.
[14]FU D,SZCS P,YAN L,et al. Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat[J]. Molecular Genetics and Genomics,2005,274(4):442-443.
[15]VON ZITZEWITZ J,SZUCS P,DUBCOVSKY J,et al. Molecular and structural characterization of barley vernalization genes[J]. Plant Molecular Biology,2005,59(3):449-467.
[16]DISTELFELD A,TRANQUILLI G,LI C,et al. Genetic and molecular characterization of the VRN2 loci in tetraploid wheat[J]. Plant Physiology,2009,149(1):245-257.
[17]NITCHER R,DISTELFELD A,TAN C,et al. Increased copy number at the HvFT1 locus is associated with accelerated flowering time in barley[J]. Molecular Genetics and Genomics:MGG,2013,288(5-6):261-275.
[18]KIPPES N,ZHU J,CHEN A,et al. Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat[J]. Molecular Genetics andGenomics: MGG,2014,289(1):47-62.
[19]HOOGENDOORN J. A reciprocal F1 monosomic analysis of the genetic control of time of ear emergence,number of leaves and number of spikelets in wheat (Triticum aestivum L.)[J]. Euphytica,1985,34(2):545-558.
[20]王丽辉,张立平,赵昌平,等. 小麦挑旗期和抽穗期的QTL分析[J]. 分子植物育种,2008,6(4):689-694.
[21]BULLRICH L,APPENDINO L,TRANQUILLI G,et al. Mapping of a thermo-sensitive earliness per se gene on Triticum monococcum chromosome 1A(m)[J]. TAG Theoretical and Applied Genetics Theoretische and Angewandte Genetik,2002,105(4):585-593.
[22]LAURIE D A,PRATCHETT N,SNAPE J W,et al. RFLP mapping of five major genes and eight quantitative trait loci controlling flowering time in a winter x spring barley (Hordeum vulgare L.) cross[J]. Genome／National Research Council Canada=Genome／Conseil National de Recherches Canada,1995,38(3):575-585.
[23]姜宗庆, 封超年, 刘萍, 等. 施磷量对不同类型专用小麦产量及剑叶相关生理特性的影响[J]. 江苏农业科学, 2013, 41(1):76-80.
[24]SHARP P J,CHAO S,DESAI S,et al. The isolation,characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm [J]. Theoretical and Applied Genetics,1989,78(3):342-348.
[25]RODER M S,KORZUN V,WENDEHAKE K,et al. A microsatellite map of wheat[J]. Genetics,1998,149(4):2007-2023.
[26]SOMERS D J,ISAAC P,EDWARDS K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.)[J]. TAG Theoretical and Applied Genetics Theoretische and Angewandte Genetik,2004,109(6):1105-1114.
[27]PAUX E,SOURDILLE P,SALSE J,et al. A physical map of the 1-gigabase bread wheat chromosome 3B[J]. Science,2008,322(5898):101-104.
[28]ELLIS H,SPIELMEYER W,GALE R,et al. ‘Perfect’ markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat[J]. TAG Theoretical and Applied Genetics Theoretische and Angewandte Genetik,2002,105(6-7):1038-1042.
[29]FU D,SZCS P,YAN L,et al. Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat [J]. Molecular Genetics and Genomics,2005,273(1):54-65.
[30]MCCOUCH S R,CHO Y G,YANO M,et al. Report on QTL nomenclature[J]. Rice Genet Newsl,1997,14:11-14.
[31]蒋彦婕, 朱芳芳, 蔡士宾, 等. 小麦纹枯病抗性 QTL 遗传分析[J]. 江苏农业学报, 2014, 30(6):1222-1226.
[32]GRIFFITHS S,SIMMONDS J,LEVERINGTON M,et al. Meta-QTL analysis of the genetic control of ear emergence in elite European winter wheat germplasm[J]. TAG Theoretical and Applied Genetics Theoretische and Angewandte Genetik,2009,119(3):383-395.
[33]ZHANG K,TIAN J,ZHAO L,et al. Detection of quantitative trait loci for heading date based on the doubled haploid progeny of two elite Chinese wheat cultivars[J]. Genetica,2009,135(3):257-265.
[34]SOURDILLE P,CADALEN T,GUYOMARCH H,et al. An update of the Courtot x Chinese Spring intervarietal molecular marker linkage map for the QTL detection of agronomic traits in wheat[J]. TAG Theoretical and Applied Genetics Theoretische and Gngewandte Genetik,2003,106(3):530-538.
[35]SHINDO C,TSUJIMOTO H,SASAKUMA T. Segregation analysis of heading traits in hexaploid wheat utilizing recombinant inbred lines[J]. Heredity,2003,90(1):56-63.
[36]HANOCQ E,NIARQUIN M,HEUMEZ E,et al. Detection and mapping of QTL for earliness components in a bread wheat recombinant inbred lines population[J]. TAG Theoretical and Applied Genetics Theoretische and Angewandte Genetik,2004,110(1):106-115.
[37]宋彦霞,景蕊莲,霍纳新,等. 普通小麦(T. aestivnm L.)不用作图群体抽穗期QTL分析[J]. 中国农业科学, 2006,39(11):2186-2193.
[38]BENNETT D,IZANLOO A,EDWARDS J,et al. Identification of novel quantitative trait loci for days to ear emergence and flag leaf glaucousness in a bread wheat (Triticum aestivum L.) population adapted to southern Australian conditions[J]. TAG Theoretical and Applied Genetics Theoretische and Angewandte Genetik,2012,124(4):697-711.

备注/Memo

备注/Memo:
收稿日期：2015-07-29 基金项目：江苏省自然科学基金青年基金项目(BK20130457);扬州市2014自然科学基金面上项目(YZ2014067);江苏省农业科技自主创新基金项目[CX(13)2022];现代农业产业技术体系建设专项基金项目(CARS-3-1-1);国家科技支撑计划项目(2011BAD35B03) 作者简介：吴旭江(1982-),男,江苏盐城人,博士,助理研究员,主要从事小麦遗传育种研究。 通讯作者：张勇,(E-mail)wheat@wheat.org.cn
更新日期/Last Update: 2015-12-31