[1]纪剑辉,周颖君,杨雯,等.水稻Dof转录因子家族的鉴定与生物信息学分析[J].江苏农业学报,2015,(06):1191-1198.[doi:doi:10.3969/j.issn.1000-4440.2015.06.001]
 JI Jian-hui,ZHOU Ying-jun,YANG Wen,et al.Identification and bioinformatics analysis of Dof transcription factors family in rice[J].,2015,(06):1191-1198.[doi:doi:10.3969/j.issn.1000-4440.2015.06.001]
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水稻Dof转录因子家族的鉴定与生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1191-1198
栏目:
遗传育种·生理生化
出版日期:
2015-12-31

文章信息/Info

Title:
Identification and bioinformatics analysis of Dof transcription factors family in rice
作者:
纪剑辉周颖君杨雯谢晶华慧杨立明
(淮阴师范学院生命科学学院/江苏省环洪泽湖生态农业生物技术重点实验室/江苏省区域现代农业与环境保护协同创新中心,江苏淮安223300)
Author(s):
JI Jian-huiZHOU Ying-junYANG WenXIE JingHUA HuiYANG Li-ming
(School of Life Sciences,Huaiyin Normal University/Jiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake/Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environment Protection 〈JCIC-RMAEP〉,Huai′an 223300,China)
关键词:
水稻Dof转录因子家族进化分析
Keywords:
riceDof transcription factorphylogenetic analysis
分类号:
S511.035.3
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.001
文献标志码:
A
摘要:
为了在全基因组水平上鉴定水稻Dof转录因子家族基本生物学特征,本研究对30个水稻Dof转录因子进行了聚类及功能结构域的分析。结合蛋白质氨基酸序列比对结果,明确了该家族所有基因均具有高度保守的Dof结构域;此外,对水稻、拟南芥、二穗短柄草和高粱中Dof家族同源基因的聚类分析表明该家族基因在植物中具有高度同源性。利用MeMe程序进一步分析了水稻Dof转录因子家族的基序特征,结果与聚类分析具有一致性;通过对染色体复制情况的研究,明确了部分Dof基因在水稻本身以及水稻与其他物种之间存在种内和种间的局部染色体复制;结合生物芯片数据,进一步研究了水稻Dof基因在不同组织中的表达,结果显示部分Dof基因在叶片或根、茎部位呈现高表达,部分Dof基因在生殖器官呈现高表达,推测这些Dof基因可能参与这些特定组织的发育。
Abstract:
For the genome-wide identification of rice Dof transcription factors,the functional domain of 30 rice Dof genes were clustered and analysed. Together with the protein sequences alignment,it was comfirmed that all Dof genes contained the highly conserved domain. Clustering analysis revealed the Dof genes were highly homologous in rice and other plants(Arabidopsis,Brachypodium distachyom and Sorghum bicolor). The MEME-defined motif characteristic of Dof genes family was in accordance with the results of protein clusting analysis. The existence of the intraspecific and interspecific chromosome replication between rice and other plants was confirmed by chromosome replication analysis. The expression pattern of rice Dof genes in different tissues showed that that some were upregulated in leaves,roots or stems,and some were upregulated in reproductive organs, suggesting that DOf genes might play roles in the development of specific organs.

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备注/Memo

备注/Memo:
收稿日期:2015-07-24 基金项目:国家自然科学基金项目 (31400169、30900871);江苏省自然科学基金项目(BK20140454、BK2011409);江苏高校品牌专业建设工程项目(PPZY2015A018);淮安市科技计划项目(国际科技合作研究)(HAC2014012);江苏省高校“青蓝工程”项目 作者简介:纪剑辉(1981-),男,江苏南通人,博士,讲师,研究方向为水稻分子生物学。(Tel)0517-83525993;(E-mail)jijianhui@hytc.edu.cn。周颖君为共同第一作者。 通讯作者:杨立明,(E-mail)yanglm@hytc.edu.cn
更新日期/Last Update: 2015-12-31