[1]程亮,郭青云.内生真菌HL-1的除草活性及对作物的安全性[J].江苏农业学报,2015,(05):1012-1016.[doi:doi:10.3969/j.issn.1000-4440.2015.05.011]
　CHENG Liang,GUO Qing-yun.Herbicidal activity of fungal endophyte HL-1 against weeds and its safety to crops[J].,2015,(05):1012-1016.[doi:doi:10.3969/j.issn.1000-4440.2015.05.011]
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内生真菌HL-1的除草活性及对作物的安全性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1012-1016
栏目:
植物保护
出版日期:
2015-10-31

文章信息/Info

Title:
Herbicidal activity of fungal endophyte HL-1 against weeds and its safety to crops
作者:
程亮12; 郭青云12
（1.青海省农林科学院植物保护研究所/青海省农业有害生物综合治理重点实验室,青海西宁810016；2.农业部西宁作物有害生物科学观测实验站,青海西宁810016）
Author(s):
CHENG Liang12; GUO Qing-yun12
(1.Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province/Institute of Plant Protection, Qinghai Academy of Agriculture and Forestry Science, Xining 810016, China;2.Scientific Observation and Experimental Station of Crop Pest in Xining, Ministry of Agriculture, Xining 810016, China)〖JP〗
关键词:
内生真菌; 杂草; 除草活性; 作物安全性
Keywords:
fungal endophyte; filtrate; weed control; herbicidal activity; safety to crop
分类号:
S482.4+9
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.011
文献标志码:
A
摘要:
从青海省化隆县发病刺儿菜叶片上分离获得菌株HL-1，其发酵滤液对供试阔叶杂草猪殃殃、藜、冬葵、酸模叶蓼的发病率分别为98.10%、90.20%、56.67%和66.67%，病情指数为98.01、88.33、56.71和47.46；鲜质量防效达到69.31%、71.83%、65.54%和56.10%，表现出较好的除草活性。作物安全性试验结果显示，其发酵滤液对小麦、蚕豆安全，对油菜、豌豆和青稞有轻微影响。对该菌株的培养特性和16S rDNA基因序列进行分析，确定其为内生真菌。内生真菌菌株HL-1可以在以猪殃殃和藜为优势杂草的春小麦和蚕豆田中作为微生物源除草剂使用。
Abstract:
Strain HL-1 isolated from diseased Cirsium setosum (Willd.) MB. leaves in Hualong, Qinghai province, was evaluated for its herbicidal activity to target weeds and its safety to crops. The disease incidences of Galium aparine L., Chenopodium album L., Malva crispa L., and Polygonum lapathifolium L.applied with HL-1 filtrate were 98.10%, 90.20%, 56.67%, and 66.67%, the disease indexes were 98.01, 88.33, 56.71, and 47.46, and the fresh weight reductions were 69.31%, 71.83%, 65.54%, 56.10%, respectively. The filtrate was safe to wheat and broad bean and slightly pathogenic to rapeseed, pea and highland barly. The strain was identified as fungal endophyte by cultural characteristic and 16SrDNA sequencing. These results indicated that fungal endophyte HL-1 could be a potential microbial herbicide for the control of G. aparine L. and C. album L. dominating in wheat and broad bean fields.

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备注/Memo

备注/Memo:
收稿日期：2015-04-15 基金项目：国家高技术研究发展计划（863计划）项目（2011AA10A206）；国家自然科学基金项目（30860165、31160371）;国家“十二五”科技支撑计划（2012BAD19B02）〖JP〗 作者简介：程亮（1978-），男，河南林州市人，硕士，副研究员，主要从事农田杂草生物防治研究。(Tel)0971-5313283；(E-mail)liangcheng1979@163.com
更新日期/Last Update: 2015-10-31