[1]苏芃,Nguyen Hac Hien,管苇,等.辐射花粉授粉技术诱导黄瓜单倍体[J].江苏农业学报,2015,(01):134-137.[doi:10.3969/j.issn.1000-4440.2015.01.021]
　SU Peng,Nguyen Hac Hien,GUAN Wei,et al.Induction of haploid cucumbers by irradiated pollen pollination[J].,2015,(01):134-137.[doi:10.3969/j.issn.1000-4440.2015.01.021]
点击复制

辐射花粉授粉技术诱导黄瓜单倍体()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
134-137
栏目:
园艺
出版日期:
2015-02-28

文章信息/Info

Title:
Induction of haploid cucumbers by irradiated pollen pollination
作者:
苏芃; Nguyen Hac Hien; 管苇; 崔利; 陈劲枫
（南京农业大学园艺学院，作物遗传与种质创新国家重点实验室,江苏南京210095）
Author(s):
SU Peng; Nguyen Hac Hien; GUAN Wei; CUI Li; CHEN Jin-feng
（College of Horticulture, Nanjing Agricultural University, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China）
关键词:
黄瓜(Cucumis sativus L.); 辐射花粉; 单倍体; 植株再生
Keywords:
cucumber (Cucumis sativus L.); pollen irradiation; haploid; plant regeneration
分类号:
S642.2
DOI:
10.3969/j.issn.1000-4440.2015.01.021
文献标志码:
A
摘要:
辐射花粉授粉是获得单倍体的有效途径。本研究旨在探讨该途径中影响单倍体再生率的关键因素。本试验利用60Coγ对不同黄瓜材料进行不同剂量的辐射处理，系统研究了辐射剂量、花粉供体基因型、母本基因型对诱导黄瓜单倍体植株再生率的影响。结果表明：3个花粉供体基因型（5211、CC3、南抗一号）花粉经150 Gy辐射处理后，花粉供体基因型5211诱导的植株再生率、单倍体再生率均高于花粉供体基因型CC3和南抗一号。花粉供体在辐射剂量150 Gy辐射处理下较250 Gy及350 Gy更有利于诱导植株再生，再生率最高可达3.92%，单倍体再生率为1.96%。10个母本基因型中6个母本基因型没有诱导出再生植株,占母本基因型总量的60%;4个母本基因型诱导出再生植株,占母本基因型总量的40%，单倍体诱导率为 0.26～0.54。
Abstract:
Irradiated pollen pollination combining embryo rescue is one of the effective ways to induce haploid and double haploid (DH). To figure out the factors affecting haploid induction, 20 different cucumber genotypes were used as maternal parents, and 3 different cucumber genotypes employed as the paternal parents were irradiated with 3 dosages (150 Gy, 250 Gy, 350 Gy) of 60Coγ. The results that the plant regeneration rate in 150 Gy treatment was higher than those in 250 Gy and 300 Gy treatments. The pollen donar 5211 in 150 Gy treatment showed the highest plant regeneration rate of 3.92% and haploid regeneration rate of 1.96%. Six maternal genotypes accounting for 60% of all maternal genotypes failed to induce plantlets, and other four genotypes regenerated plantlets, with haploid induction rate ranging from 0.26 to 0.54.

参考文献/References:

[1]吴传万,杜小凤,顾大路,等. 植物源药肥对温室黄瓜生长发育和土壤环境的影响[J]. 江苏农业学报,2014,30(1):92-99.
[2]张黎杰，周玲玲，李志强，等．菌渣复合基质栽培对目光温室黄瓜生长发育和产量品质的影响[J]．江苏农业科学，2014，42(3)：109-111.
[3]费雨兰，王晶，沈佳，等．不同砧木嫁接对黄瓜长势及果实品质的影响[J]．江苏农业科学，2013，41(12)：147-149.
[4]刘琴，王艳，徐健，等．枯草芽孢杆菌Bs-1013的抑菌活性及其包衣对黄瓜发芽生长的影响[J]．江苏农业科学，2013，41(11)：117-119.
[5]KENNARD W C, POTTER K, DIJKHUIZEN A, et al. Linkages among RFLP,RAPD, isozyme, disease resistance, and morphological markers in narrow and wide crosses of cucurnber [J]. Theoretical and Applied Genetics, 1994, 89:42-48.
[6]BRADENN J M, STAUB J E, WYE C, et al. Towards an expanded and integrated linkage map of cucumber (Cucumis sativus L.) [J].Genome, 2001, 44:111-119.
[7]李加旺,张文珠,何忠魁. 黄瓜资源筛选与育种研究及其发展趋势[J]. 天津农业科学,1999, 5(4):36-38.
[8]张献龙. 植物生物技术[M]. 北京: 科学出版社, 2004:35-69.
[9]SAUTON. A,DUMAS DE VAULX R. Obtention de plantes haplids chezle melon (Cucumis melon L.)aprèsgynogènèse induite pardu pollen irradiè[J]. Agronomie, 1987，7:141-148.
[10]陈劲枫,钱春桃 .利用几种园艺作物卷须制片鉴定染色体数目的研究[J]. 园艺学报, 2002,29(4): 378-380.
[11]CAGLAR. G,ABAK K. Progress in the production of haploid embryos, plants and doubled haploids in cucumber (Cucumis sativus L.) by gamma irradiated pollen in Turkey[J]. Acta Horticulturae, 1999，492:317-320.
[12]FICCADENTI N， VERONESEP, SESTILIS, et al. Influece of genotype on the inductuin of haploidy in Cucumis melo L. by using irradiated pollen[J].J Genet&Breed,1995，49:359-364.
[13]FARIS N M. The effectof gamma irradiation dose on cucumber (Cucumis sativus L.) haploid embryo production[J]. Acta Physiologiae Plantarum, 1999， 21(4):391-396.
[14]VISSER T， OSST E H. Pollen and pollination experminentsIII the viability of apple and pear pollen as affected by irradiation and storage[J]. Euphytuca，1981. 30:65-70.
[15]DENISSEN， CJM DENIJS A P M. Effects of gamma irradiation on in vitro pollen germination of different Cucumis species [J]. Euphytica, 1987， 36:651-658.
[16]SARI N AK. Induction of parthenogenetic haploid embryos after pollination by irradiated pollen in watermelon[J]. Hortscience, 1994，29(10):1189-1190.

备注/Memo

备注/Memo:
收稿日期：2014-05-09 基金项目：国家“863”计划项目（2012AA100102） 作者简介：苏芃（1989-），女，江苏扬州人，硕士研究生，研究方向为植物组织培养。（E-mail）2011104051@njau.edu.cn 通讯作者：陈劲枫，（E-mail）jfchen@njau.edu.cn
更新日期/Last Update: 2015-02-28