[1]陈夕军,朱键鑫,陈羽,等.抗白粉病黄瓜品种的叶片组织结构及其生理生化[J].江苏农业学报,2015,(01):55-61.[doi:10.3969/j.issn.1000-4440.2015.01.008]
　CHEN Xi-jun,ZHU Jian-xin,CHEN Yu,et al.Physiological and biochemical mechanism of cucumber cultivars resistant to powdery mildew and their differences in microstructure of leaves[J].,2015,(01):55-61.[doi:10.3969/j.issn.1000-4440.2015.01.008]
点击复制

抗白粉病黄瓜品种的叶片组织结构及其生理生化()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
55-61
栏目:
植物保护
出版日期:
2015-02-28

文章信息/Info

Title:
Physiological and biochemical mechanism of cucumber cultivars resistant to powdery mildew and their differences in microstructure of leaves
作者:
陈夕军1; 朱键鑫1; 陈羽1; 张青12; 张家豪1; 张孝然1; 黄奔立1
（1.扬州大学园艺与植物保护学院，江苏扬州225009；2.苏州市农业科学院，江苏苏州215155）
Author(s):
CHEN Xi-jun1; ZHU Jian-xin1; CHEN Yu1; ZHANG Qing12; ZHANG Jia-hao1; ZHANG Xiao-ran1; HUANG Ben-li1
（1.College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China;2.Suzhou Academy of Agricultural Sciences, Suzhou 215155, China）
关键词:
组织结构; 生理生化机制; 白粉病; 抗病性; 黄瓜
Keywords:
tissue structure; physiological and biochemical mechanism; powdery mildew; resistance; cucumber
分类号:
S436.421.1+2
DOI:
10.3969/j.issn.1000-4440.2015.01.008
文献标志码:
A
摘要:
为明确不同黄瓜品种对白粉病的抗性机制，采用组织切片和田间接种的方法，从近130份种质中筛选出高抗（GY14A、GY4）、中抗（津春4号、津优30号）、中感（W12757、仪黄）、高感（DE843、平望乳瓜）黄瓜品种各2份，并对8份黄瓜品种的形态结构与抗性生理生化进行研究。结果表明，高抗品种叶片组织结构紧凑，栅栏组织排列整齐、紧密，且细胞壁较厚；而高感品种则细胞壁较薄，叶片组织间出现大量空隙；中抗和中感品种虽具清晰的栅栏组织，但空隙较大，说明叶片组织细胞的排列整齐和紧密度以及细胞壁的厚度与黄瓜品种对白粉病的抗性密切相关。病原菌接种后，抗性品种体内防御酶活性上升速度与幅度都明显高于感病品种，且过氧化物酶（POD）同功酶谱带较深，H2O2积累较慢。另外，抗性品种叶片中可溶性蛋白质含量明显高于感病品种，而可溶性糖含量则明显低于感病品种，还原糖含量无明显差异。
Abstract:
To define the resistance mechanism of cucumber to powdery mildew, eight cucumber cultivars, selected from near 130 cultivars with different resistance levels to powdery mildew, were involved to research the differences of microstructures and physiological and biochemical indexes. Cucumber cultivars GY14A and GY4 are highly resistant, Jinchun No.4 and Jinyou No.30 are moderately resistant, W12737 and Yihuang are moderately susceptible, and DE843 and Pingwangrugua are highly susceptible. The highly resistant cultivars showed arranged palisade tissues thick cell walls while the highly susceptible cultivars exhibited thin cell walls and a great number of interspaces among cells. The palisade tissues were clearly seen in moderately resistant and susceptible cultivars, however, with large interspaces among the cells. The results indicated that the resistance levels of cucumber cultivars were associated with the arrangement and compactness of leaf cells and the thickness of their cell walls. After inoculation with Sphaerotheca fuliginea, the increases of defense enzymes activities of resistant cultivars were greater than those of susceptible cultivars. The bands of POD isozymes of resistant cultivars were much darker, and the accumulations of H2O2 were slower. In addition, the contents of soluble proteins in the resistant cultivars were higher than those in the susceptible cultivars while the soluble sugar contents were lower. The reducing sugar contents had no obvious difference.

参考文献/References:

[1]冯东昕,李宝栋. 主要瓜类作物抗白粉病育种研究进展 [J]. 中国蔬菜, 1996(1): 55-59.
[2]李海英,倪红涛,杨庆凯. 大豆叶片结构与灰斑病抗性的研究Ⅰ: 大豆叶片气孔密度、茸毛密度与灰斑病抗性的关系 [J]. 中国油料作物学报, 2001, 23(3): 52-53.
[3]李海英,刘亚光,杨庆凯. 大豆叶片结构与灰斑病抗性的研究Ⅱ: 大豆叶片组织结构与灰斑病抗性的关系 [J]. 中国油料作物学报, 2002, 24(2): 58-60.
[4]FORD C M. Identification of seedless table grape cultivars and a bud sport berry [J]. Horticulture Science, 1993, 17(3): 366-368.
[5]NAGDY P L, BOYD D J. Binary pathway for analysis of primary infection and host response in populations of powdery mildew fungi [J]. Canadian Journal of Botany, 1993, 57: 497-511.
[6]沙爱华,黄俊斌,林兴华,等. 水稻白叶枯病成株抗性与过氧化氢含量及几种酶活性变化的关系 [J]. 植物病理学报, 2004, 34(4): 340-345.
[7]KIM KHIOOK I L, SCHNEIDER C, HELOIR M C, et al. Image analysis methods for assessment of H2O2 production and Plasmopara viticola development in grapevine leaves: application to the evaluation of resistance to downy mildew [J]. Journal of Microbiological Methods, 2013, 95(2): 235-244.
[8]马雪瑞,段玉玺,陈立杰,等. 利用抗坏血酸揭示小粒黑豆对胞囊线虫抗性的研究 [J]. 大豆科学, 2011, 30(1): 123-126.
[9]KERCHEV P I, PELLNY T K, VIVANCOS P D, et al. The transcription factor ABI4 is required for the ascorbic acid-dependent regulation of growth and regulation of jasmonate-dependent defense signaling pathways in Arabidopsis [J]. The Plant Cell, 2011, 23: 3319-3334.
[10]陈喜文,郝友进,陈德富,等. 含氮杂环化合物对黄瓜白粉病抗性的诱导作用及其与防御酶系统的关系 [J]. 植物病理学报, 2003, 33(6): 535-540.
[11]郭红莲,程根武,陈捷,等. 玉米灰斑病抗性酚类物质代谢作用的研究 [J]. 植物病理学报, 2003, 33(4): 342-346.
[12]吕淑珍,马德华,霍振荣,等. 优质抗病高产黄瓜新品种－津春4号 [J]. 中国蔬菜, 1994(2): 1-3.
[13]蔡建华,黄奔立,朱键鑫,等. 黄瓜不同种质材料白粉病田间发病调查及抗性分析 [J]. 江苏农业科学, 2006(6): 152-155.
[14]陈夕军,沈世炜,陈银凤,等. 氯化胆碱诱导黄瓜抗白粉病机理研究 [J]. 中国蔬菜, 2013(18):81-87.
[15]方中达. 植病研究方法 [M]. 北京: 中国农业出版社, 1998:91-108.
[16]孔凡明,许志刚. 水稻不育系抗白枯病与体内酶活性变化的关系 [J]. 安徽农业大学学报, 1998, 25(3): 217-223.
[17]邹琦. 植物生理学实验指导 [M]. 北京: 中国农业出版社, 2000:163-164.
[18]张妙霞,孔祥生,郭秀璞,等. 蒽酮法测定可溶性糖显色条件的研究 [J]. 洛阳农专学报, 1997, 17(4): 24-28.
[19]袁晓华,杨中汉. 植物生理生化实验 [M]. 北京: 高等教育出版社, 1993:108.
[20]波钦诺克X H. 植物生物化学分析方法 [M]. 荆家海, 丁钟荣, 译. 北京: 科学出版社, 1981: 178-181.
[21]MARKESTEIJN L, POORTER L, PAZ H, et al. Ecological differentiation in xylem cavitation resistance is associated with stem and leaf structural traits [J]. Plant, Cell ＆ Environment, 2011, 34(1): 137-148.
[22]姜淑苓,贾敬贤,王斐,等. 早金香梨抗黑星病鉴定及组织结构与抗病关系研究 [J]. 中国农学通报, 2009, 25(4): 215-217.
[23]HAO Y H, WU C F, ZHAO D W, et al. Proteomic analysis of glucohexaose induced resistance to downy mildew in ‘Cucumis Sativus’ [J]. Australian Journal of Crop Science, 2013, 7(9): 1242-1251.
[24]CHEN F, WANG M, ZHENG Y, et al. Quantitative changes of plant defense enzymes and phytohormone in biocontrol of cucumber Fusarium wilt by Bacillus subtilis B579 [J]. World Journal of Microbiology and Biotechnology, 2010, 26(4): 675-684.
[25]TAHERI P, TARIGHI S. A survey on basal resistance and riboflavin-induced defense responses of sugar beet against Rhizoctonia solani [J]. Journal of Plant Physiology, 2011, 168(10): 1114-1122.
[26]龙书生,李亚玲,张宇宏,等. 糖分含量作为抗镰刀菌茎腐病玉米品种的育种指标研究 [J]. 山东农业大学学报, 1999, 30(4): 372-376.
[27]云兴福. 黄瓜组织中氨基酸、糖和叶绿素含量与其对霜霉病抗性的关系 [J]. 华北农学报, 1993, 8(4): 52-58.
[28]景岚,王丽芳,康俊. 不同抗性的向日葵品种接种锈菌后叶片中可溶性蛋白、可溶性总糖及叶绿素含量的变化 [J]. 临沂师范学院学报, 2008, 30(6): 76-80.
[29]周博如,李永镐,刘太国,等. 不同抗性的大豆品种接种大豆细菌性疫病菌后可溶性蛋白、总糖含量的变化研究 [J]. 大豆科学, 2000, 10(2): 111-114.

相似文献/References:

[1]张坤,吴海虹,邹烨,等.超声波联合一磷酸腺苷（AMP）对鸡胸肉的嫩化效果[J].江苏农业学报,2018,(06):1362.[doi:doi:10.3969/j.issn.1000-4440.2018.06.023]
　ZHANG Kun,WU Hai-hong,ZOU Ye,et al.Optimization of ultrasonic treatment and 5′-adenosine monophosphate (AMP) on the tenderness of chicken breast[J].,2018,(01):1362.[doi:doi:10.3969/j.issn.1000-4440.2018.06.023]

备注/Memo

备注/Memo:
收稿日期：2014-07-07 基金项目：江苏省农业科技自主创新基金项目[CX(12)3019]；江苏省自然科学基金项目（BK2010305） 作者简介：陈夕军（1973-），男，江苏阜宁人，博士，副教授，专业方向为植物病害及其防控。（E-mail）xjchen@yzu.edu.cn 通讯作者：黄奔立，（E-mail）yzhbl2003@163.com；张青，（E-mail） zhangqingsz@163.com
更新日期/Last Update: 2015-02-28