[1]管莉,张阿英.CaM 与 ZmCCaMK 相互作用参与 BR 诱导的玉米叶片抗氧化防护[J].江苏农业学报,2015,(01):10-15.[doi:10.3969/j.issn.1000-4440.2015.01.002]
　GUAN Li,ZHANG A-ying.CaM-ZmCCaMK interaction involved in brassinosteroid-induced antioxidant defense in leaves of maize[J].,2015,(01):10-15.[doi:10.3969/j.issn.1000-4440.2015.01.002]
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CaM 与 ZmCCaMK 相互作用参与 BR 诱导的玉米叶片抗氧化防护()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
10-15
栏目:
遗传育种·耕作栽培·生理生化
出版日期:
2015-02-28

文章信息/Info

Title:
CaM-ZmCCaMK interaction involved in brassinosteroid-induced antioxidant defense in leaves of maize
作者:
管莉; 张阿英
（南京农业大学生命科学学院，江苏南京210095）
Author(s):
GUAN Li; ZHANG A-ying
（College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China）
关键词:
玉米; 油菜素内酯; 钙调素; 抗氧化酶; H2O2; ZmCCaMK
Keywords:
maize (Zea mays L.); brassinosteroid; calmodulin; antioxidant enzyme; hydrogen peroxide; ZmCCaMK
分类号:
S513.01
DOI:
10.3969/j.issn.1000-4440.2015.01.002
文献标志码:
A
摘要:
以玉米（Zea mays L.）为材料，通过酶联免疫法、实时荧光定量PCR技术、免疫共沉淀激酶反应以及原生质体瞬时表达体系等方法研究了在油菜素内酯（Brassinosteroids, BR）诱导的玉米叶片抗氧化防护过程中钙调素（Calmodulin, CaM）的作用以及CaM和Ca2+/CaM依赖的蛋白激酶（ZmCCaMK）之间的关系。结果显示：外源BR处理显著提高了玉米叶片叶肉细胞原生质体中的CaM含量，CaM拮抗剂的预处理几乎完全抑制了BR诱导的抗氧化防护酶活性，表明CaM参与了BR诱导的抗氧化防护。而且ZmCCaMK原生质体瞬时表达及瞬时沉默结果显示ZmCCaMK影响BR诱导的CaM含量，CaM拮抗剂的预处理也显著抑制了BR诱导的H2O2的产生、ZmCCaMK基因表达以及激酶活性。说明BR诱导产生的CaM增强H2O2的积累，进一步诱导抗氧化防护酶活性增加，并且CaM和ZmCCaMK之间存在互动机制。
Abstract:
In this study, enzyme-linked immunosorbent assay (ELISA), real-time PCR, immunoprecipitation and in-gel kinase assay and protoplast transient expression system were used to investigate the role of calmodulin (CaM) in brassinosteroids (BR)-induced antioxidant defense and the relationship between CaM and Ca2+/CaM-dependent protein kinase (ZmCCaMK) in leaves of maize (Zea mays L.) plants. The results showed that treatment with BR led to significant increase in the content of CaM in protoplasts of maize plants and pre-treatment with CaM antagonists blocked the activities of antioxidant enzymes induced by BR treatment in leaves of maize, which suggest that CaM is involved in BR-induced antioxidant defense. Moreover, transient expression or transient silencing of ZmCCaMK in protoplasts resulted in a significant enhancement or block in the content of CaM induced by BR treatment. Pre-treatment with CaM antagonists significant inhibited the production of H2O2, the expression and the activity of ZmCCaMK induced by BR in the leaves of maize. The results suggest that the BR-induced CaM increased H2O2 accumulation, increased the activities of antioxidant enzymes, and a cross-talk may exist between CaM and ZmCCaMK in BR signaling in the leaves of maize plants.

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备注/Memo

备注/Memo:
收稿日期：2014-05-20 基金项目：教育部新世纪优秀人才支持计划项目（NCET-10-0498） 作者简介：管莉（1987-），女，吉林白山人，硕士研究生，主要从事植物生理学研究。（Tel）18260081503; (E-mail) guanli9487@163.com 通讯作者：张阿英，（Tel）025-84396372; (E-mail) ayzhang@njau.edu.cn
更新日期/Last Update: 2015-02-28