[1]郭梦鸽,秦孝天,陈瑞丹.6个朱砂梅品种花色苷合成结构基因及转录因子编码基因的表达模式分析[J].江苏农业学报,2024,(02):367-375.[doi:doi:10.3969/j.issn.1000-4440.2024.02.019]
　GUO Meng-ge,QIN Xiao-tian,CHEN Rui-dan.Analysis of the expression pattern of structural genes and transcription factors encoding genes related to the anthocyanin synthesis in six cultivars of Prunus mume Cinnabar Purple Group[J].,2024,(02):367-375.[doi:doi:10.3969/j.issn.1000-4440.2024.02.019]
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6个朱砂梅品种花色苷合成结构基因及转录因子编码基因的表达模式分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年02期
页码:
367-375
栏目:
园艺
出版日期:
2024-02-25

文章信息/Info

Title:
Analysis of the expression pattern of structural genes and transcription factors encoding genes related to the anthocyanin synthesis in six cultivars of Prunus mume Cinnabar Purple Group
作者:
郭梦鸽1; 秦孝天1; 陈瑞丹1; 2; 3; 4; 5; 6
(1.北京林业大学园林学院,北京100083；2.花卉种质创新与分子育种北京市重点实验室,北京100083；3.国家花卉工程技术研究中心,北京100083；4.城乡生态环境北京实验室,北京100083；5.园林环境教育部工程研究中心,北京100083；6.林木花卉遗传育种教育部重点实验室,北京100083)
Author(s):
GUO Meng-ge1; QIN Xiao-tian1; CHEN Rui-dan1; 2; 3; 4; 5; 6
(1.School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China；2.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing 100083, China；3.National Engineering Research Center for Floriculture,Beijing 100083, China；4.Beijing Laboratory of Urban and Rural Ecological Environment, Beijing 100083, China；5.Engineering Research Center of Landscape Environment of Ministry of Education, Beijing 100083, China；6.Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing 100083, China)
关键词:
梅花; 花色苷; 结构基因; 转录因子; 荧光定量PCR
Keywords:
Prunus mume; anthocyanin; structural genes; transcription factors; qRT- PCR
分类号:
S685.17
DOI:
doi:10.3969/j.issn.1000-4440.2024.02.019
摘要:
为明晰朱砂梅品种群梅花花色表型差异形成的分子调控机制,以花色由浅至深呈梯度变化的6个朱砂梅品种为试验材料,利用色差仪和分光光度计测定不同品种盛花期花瓣的花色表型及花色苷含量,通过荧光定量PCR及相关性分析,对花色苷合成结构基因(PmCHS、PmCHI、PmF3H、PmF3′H、PmDFR、PmANS、PmUFGT)及转录因子编码基因(PmMYB1、PmbHLH3)的转录特性进行探究。结果表明,各品种间花瓣花色苷含量与花色红度的变化趋势一致；PmMYB1、PmbHLH3、PmF3′H、PmDFR、PmUFGT的相对表达量与花色苷含量呈极显著正相关；转录因子编码基因PmMYB1与PmbHLH3的相对表达量呈极显著正相关,且均与结构基因PmF3′H、PmDFR、PmUFGT的表达水平呈显著或极显著正相关。推测转录因子编码基因PmMYB1PmbHLH3通过正向调控结构基因PmF3′H、PmDFR和PmUFGT的表达从而调控朱砂梅花瓣的呈色。
Abstract:
To clarify the molecular regulatory mechanism for the formation of phenotypic differences in flower color of Prunus mume Cinnabar Purple Group, six varieties of P. mume Cinnabar Purple Group with gradient changes in flower color from light to dark were used as the test materials. The floral color phenotypes and anthocyanin contents of petals of different varieties at full-bloom stage were determined by colorimeter and spectrophotometer, respectively. The transcriptional characteristics of the structural genes for anthocyanin synthesis (PmCHS, PmCHI, PmF3H, PmF3′H, PmDFR, PmANS, PmUFGT) and the transcription factors encoding genes (PmMYB1, PmbHLH3) were analyzed by qRT-PCR and correlation analysis. The results showed that the change trend of anthocyanin content in petals among the varieties was consistent with that of flower color redness. The relative expression of PmMYB1, PmbHLH3, PmF3′H, PmDFR and PmUFGT were highly significantly and positively correlated with anthocyanin content. The relative expression of transcription factors encoding genes PmMYB1 and PmbHLH3 were highly significantly and positively correlated, and all of them were significantly or highly significantly and positively correlated with the expression levels of the structural genes PmF3′H, PmDFR and PmUFGT. It is hypothesized that the transcription factors encoding genes PmMYB1 and PmbHLH3 regulate the expression of the structural genes PmF3′H, PmDFR and PmUFGT and thus regulate the color presentation of the petals of P. mume Cinnabar Purple Group.

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备注/Memo

备注/Memo:
收稿日期：2023-02-17基金项目：北京园林绿化增彩延绿科技创新工程项目(2019KJC0210)作者简介：郭梦鸽(1998-),女,河南平顶山人,硕士研究生,研究方向为花卉种质资源与遗传育种。(E-mail)513735355@qq.com通讯作者：陈瑞丹,(E-mail)chenruidan@163.com
更新日期/Last Update: 2024-03-17