[1]王凤婷,靳盼盼,刘芳,等.食源性阴沟肠杆菌生物膜形成能力[J].江苏农业学报,2018,(04):943-948.[doi:doi:10.3969/j.issn.1000-4440.2018.04.033]
　WANG Feng-ting,JIN Pan-pan,LIU Fang,et al.Biofilm formation ability of a food-borne Enterobacter cloacae[J].,2018,(04):943-948.[doi:doi:10.3969/j.issn.1000-4440.2018.04.033]
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食源性阴沟肠杆菌生物膜形成能力()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
943-948
栏目:
加工贮藏·质量安全
出版日期:
2018-08-25

文章信息/Info

Title:
Biofilm formation ability of a food-borne Enterobacter cloacae
作者:
王凤婷1; 2; 靳盼盼1; 2; 刘芳1; 孙芝兰1; 王道营1; 张芳3; 许晓曦2; 徐为民1
（1.江苏省农业科学院农产品加工研究所，江苏南京210014；2.东北农业大学食品学院，黑龙江哈尔滨150030；3.江苏省农业科学院中心实验室，江苏南京210014）
Author(s):
WANG Feng-ting1; 2; JIN Pan-pan1; 2; LIU Fang1; SUN Zhi-lan1; WANG Dao-ying1; ZHANG Fang3; XU Xiao-xi2; XU Wei-min1
(1.Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.College of Food Science, Northeast Agricultural University, Harbin 150030, China；3.Central Laboratory, Jiangsu Academy of Agricultural Scie
关键词:
阴沟肠杆菌; 生物膜; 胞外分泌物质
Keywords:
Enterobacter cloacae; biofilm; the secretion of extracellar polymeric substances
分类号:
Q939.121
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.033
文献标志码:
A
摘要:
本试验对从冰鲜鸡肉中分离得到的1株阴沟肠杆菌成膜特性进行研究，通过对其在培养12 h、24 h、48 h、72 h后细菌总数的变化，微观结构观察及胞外多糖质量浓度的测定，分析其在生物膜形成期间的变化规律，进而为抑制生物膜形成的杀菌剂的研究提供基础。结果表明，阴沟肠杆菌C4在培养过程中菌数增长较快，成膜迅速，12 h后细菌总数无显著（P>0.05）变化；扫描电镜和激光共聚焦显微镜的观察结果及胞外多糖质量浓度变化结果表明，12 h至48 h细胞逐渐聚集成团，相互之间联系紧密，胞外分泌物质增多将细菌包裹，72 h胞外分泌物质减少，细胞集团间空隙减少，形成致密结构。因此，在生物膜抑制的研究中可着重关注减少胞外物质的分泌，使细菌细胞裸露在外，有助于生物膜内细菌的杀死。
Abstract:
The biofilm-forming process of Enterobacter cloacae isolated from iced chicken meat was studied, and the changes of total bacteria, micro-structure and concentration of exopolysaccharide at 12 h, 24 h, 48 h and 72 h were investigated, the changes in the formation of biofilm were analyzed to provide a basis for the study of fungicides which could inhibit biofilm formation. The results showed that the process of biofilm formation for C4 was rapidly and there was no significant variation for total bacteria after 12 hours, cells gathered into a mass from 12 h to 48 h, extracellular polymeric substance(EPS) increased and bacteria cells embedded on biofilm, EPS and lacuna among cell clusters decreased after 72 hours, bacteria cells were exposed outside, forming compact structure visualized by scanning electron microscope(SEM) and confocal laser scanning microscopy(CLSM). Therefore, in the study of biofilm inhibition, more attention should be paid to decreasing the secretion of EPS, making the bacterial cells exposed, it’s useful for killing bacteria in biofilm.

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备注/Memo

备注/Memo:
收稿日期：2017-11-14 基金项目：国家自然科学基金面上项目（31371802）；江苏省自然科学基金面上项目（BK20151367）;江苏省农业科技自主创新基金项目[CX(15)1006] 作者简介：王凤婷（1993-），女，黑龙江哈尔滨人，硕士研究生，研究方向为食品微生物。（E-mail）：526013836@qq.com 通讯作者：刘芳，（E-mail）fangliu82@163.com
更新日期/Last Update: 2018-09-04