[1]谭业平,赵静,郭霄峰,等.一株携带犬细小病毒VP2融合基因的重组狂犬病毒的构建[J].江苏农业学报,2018,(04):866-870.[doi:doi:10.3969/j.issn.1000-4440.2018.04.022]
　TAN Ye-ping,ZHAO Jing,GUO Xiao-feng,et al.Construction of a recombinant rabies virus carrying canine parvovirus VP2 fusion gene[J].,2018,(04):866-870.[doi:doi:10.3969/j.issn.1000-4440.2018.04.022]
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一株携带犬细小病毒VP2融合基因的重组狂犬病毒的构建()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
866-870
栏目:
畜牧兽医·水产养殖
出版日期:
2018-08-25

文章信息/Info

Title:
Construction of a recombinant rabies virus carrying canine parvovirus VP2 fusion gene
作者:
谭业平1; 赵静2; 郭霄峰2; 何孔旺1
（1.江苏省农业科学院兽医研究所，农业部兽用生物制品工程技术重点实验室，国家兽用生物制品工程技术研究中心，江苏南京210014；2.华南农业大学兽医学院微生物学与免疫学教研室，广东广州510642）
Author(s):
TAN Ye-ping1; ZHAO Jing2; GUO Xiao-feng2; HE Kong-wang1
(1.Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biologicals Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 21001
关键词:
狂犬病毒; 犬细小病毒VP2基因; 反向遗传技术
Keywords:
rabies virus; canine parvovirus VP2 gene; reverse genetic technology
分类号:
S852.65+5
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.022
文献标志码:
A
摘要:
为构建预防狂犬病毒和犬细小病毒的疫苗候选毒株，将编码狂犬病毒G蛋白的中和抗原表位的碱基序列插入犬细小病毒VP2基因中，再将VP2融合基因插入狂犬病毒全基因组的G和L基因之间，然后应用反向遗传操作技术构建重组狂犬病毒。直接免疫荧光染色结果显示成功获得重组狂犬病毒rHEP-rVP2；生物学特性研究结果显示重组病毒随着在BHK-21细胞上传代次数的增加，病毒滴度逐渐升高，呈现良好繁殖特性；RT-PCR结果显示携带VP2融合基因的重组病毒具有良好遗传稳定性，重组病毒免疫小鼠可诱导产生保护性抗狂犬病毒和犬细小病毒抗体。
Abstract:
To build the vaccine candidate strain of rabies virus and canine parvovirus, the canine parvovirus VP2 gene which contained the rabies virus G protein neutralizing epitope was inserted into the rabies virus genome between the G and L gene. And then a recombinant rabies virus carrying canine parvovirus VP2 fusion gene was rescued using reverse genetic technology. The successful restructuring of rabies virus rHEP-rVP2 was confirmed by indirect immunofluorescence assay with antibody against RV-N protein. The results of biological characteristics showed that as the number of passages of recombinant strains in BHK-21 increased, virus titers gradually increased, showing good reproductive characteristics. Genetic stability of recombinant virus carrying VP2 fusion gene was confirmed by RT-PCR. Immunogenicity of recombinant rabies virus rHEP-rVP2 was tested in Kunming mice. Recombinant viruses rHEP-rVP2 induced high level of rabies antibody compared with HEP-Flury and high level of antibodies against VP2, which could protect against canine parvovirus infection.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2017-10-30 基金项目：江苏省自然科学青年基金项目 (BK20130705) 作者简介：谭业平(1980-)，男，山东莱阳人，博士，副研究员，主要从事动物疫病风险评估与防控研究，(E-mail)yepingtan@163.com
更新日期/Last Update: 2018-09-04