[1]高珊,张凯,朱树华.桃果实PLDα基因的表达及生物信息学分析[J].江苏农业学报,2018,(02):418-424.[doi:doi:10.3969/j.issn.1000-4440.2018.02.028]
 GAO Shan,ZHANG Kai,ZHU Shu-hua.Bioinformatics analysis and expression of PLDα gene in peach fruits[J].,2018,(02):418-424.[doi:doi:10.3969/j.issn.1000-4440.2018.02.028]
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桃果实PLDα基因的表达及生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
418-424
栏目:
园艺
出版日期:
2018-04-25

文章信息/Info

Title:
Bioinformatics analysis and expression of PLDα gene in peach fruits
作者:
高珊张凯朱树华
(山东农业大学化学与材料科学学院,山东泰安271018)
Author(s):
GAO ShanZHANG KaiZHU Shu-hua
(College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, China)
关键词:
肥城桃PLDα基因表达基因表达调控冷信号
Keywords:
Feicheng peachPLDαgene expressionregulation of gene expressioncold signal
分类号:
S662.1
DOI:
doi:10.3969/j.issn.1000-4440.2018.02.028
文献标志码:
A
摘要:
磷脂酶D(PLD)能催化磷酸二酯键水解和碱基交换,其水解产物磷脂酸可以作为第二信使,参与植物信号转导过程。几乎在植物所有的生命阶段及对环境胁迫的应答中,PLD都起着重要的作用。本试验借助分子生物学手段,在体外构建了pET30a-PLDα重组表达载体,并成功地在表达菌株Transetta(DE3)中进行表达。研究发现PLDα蛋白氨基酸序列存在2个HKD基序,两基序间隔320个氨基酸,亲水性平衡系数为-0.417,表明PLDα蛋白为疏水性蛋白。系统进化树构建结果显示,桃果实PLDα蛋白与草莓PLDα蛋白二者亲缘关系最近;三维结构模拟存在的“洞穴”结构可以很好地解释该蛋白的疏水性。实时荧光定量PCR结果表明,低温能提高PLDα基因mRNA基因表达水平。
Abstract:
Phospholipase D (PLD) can catalyze the hydrolysis and base exchange of phosphodiester bonds, its hydrolyzate phosphatidic acid (PA) can be used as a second messenger to participate in plant signal transduction processes. PLD plays an important role in almost all stages of plant life and responses to environmental stress. In this study, the recombinant PLDα protein was expressed in Escherichia coli by molecular biology method. There were two HKD motifs in PLDα protein, which were separated by 320 amino acids. The hydrophilic balance coefficient was -0.417, which indicated that PLDα protein was a hydrophobic protein. The results of phylogenetic tree revealed that PLDα protein of peach was more closely related to strawberry PLDα protein in evolution. The three-dimensional structure of peach PLDα protein showed a “cave” structure, this structure could explain the hydrophobicity of the protein. The results of qPCR showed that PLDα mRNA of peach fruit was induced in the low temperature.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-08-28 基金项目:国家自然科学基金项目(31470686) 作者简介:高珊(1990-),女,山东潍坊人,硕士研究生,主要从事植物分子生物学研究。(E-mail)18205480669@163.com 通讯作者:朱树华,(E-mail)shuhua@sdau.edu.cn
更新日期/Last Update: 2018-05-04