[1]张蕾,张响英,章敬旗,等.高、低产双黄蛋高邮鸭卵巢结构及生殖激素和相关基因差异[J].江苏农业学报,2018,(02):385-389.[doi:doi:10.3969/j.issn.1000-4440.2018.02.023]
　ZHANG Lei,ZHANG Xiang-ying,ZHANG Jing-qi,et al.Differentiation of ovary structure, reproductive hormones and related genes expression between high and low double-yolk egg laying Gaoyou duck[J].,2018,(02):385-389.[doi:doi:10.3969/j.issn.1000-4440.2018.02.023]
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高、低产双黄蛋高邮鸭卵巢结构及生殖激素和相关基因差异()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
385-389
栏目:
畜牧兽医·水产养殖
出版日期:
2018-04-25

文章信息/Info

Title:
Differentiation of ovary structure, reproductive hormones and related genes expression between high and low double-yolk egg laying Gaoyou duck
作者:
张蕾1; 张响英1; 章敬旗1; 张扬2; 常国斌2; 陈国宏2; 董飚1; 吴炳鑫1; 王健1
（1.江苏农牧科技职业学院，江苏泰州225300;2.扬州大学动物科学与技术学院，江苏扬州225009）
Author(s):
ZHANG Lei1; ZHANG Xiang-ying1; ZHANG Jing-qi1; ZHANG Yang2; CHANG Guo-bin2; CHEN Guo-hong2; DONG Biao1; WU Bing-xin1; WANG Jian1
（1.Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;2.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China）
关键词:
高邮鸭; 双黄蛋; 卵巢形态; 基因表达; 生殖激素
Keywords:
Gaoyou duck; double-yolk egg; ovary structure; gene expression; reproductive hormone
分类号:
S834.2
DOI:
doi:10.3969/j.issn.1000-4440.2018.02.023
文献标志码:
A
摘要:
为从组织学、生化指标及分子水平研究双黄蛋高、低产高邮鸭卵巢结构、生殖激素和繁殖关键基因的差异性，选择双黄蛋高、低产高邮鸭各6只，测定静脉血中相关激素含量，采用qRT-PCR技术检测下丘脑、垂体、肝脏以及卵巢组织中相关基因的表达，并对卵巢组织切片进行HE染色和FSHR免疫组化染色，观察卵巢形态结构。结果表明，高邮鸭双黄蛋高、低产组卵巢形态结构有显著差异，高产组卵巢卵泡发育良好，内外膜分界明显，颗粒细胞排列紧密；低产组卵巢卵泡发育不良，颗粒细胞松散。高产组血清中促卵泡素（FSH）、雌二醇（E2）和孕酮（P）水平显著高于低产组（P<0.01）。qRT-PCR结果显示，高、低产高邮鸭下丘脑、垂体及肝脏中生殖激素受体基因FSHR、ERβ和GnRHR的mRNA表达存在极显著性差异（P<0.01），而在卵巢中差异不显著。可见，双黄蛋高、低产高邮鸭的卵巢结构存在显著差异，下丘脑、垂体以及肝脏是调控高邮鸭双黄蛋产蛋性能的重要因素。
Abstract:
High and low double-yolk egg laying Gaoyou ducks were used as the experiment material to explore the differences of ovary structure, reproductive hormones and related genes expression from the morphology, biochemical indicators and molecular level. Total 12 Gaoyou ducks were chosen from high and low double-yolk egg laying groups, and venous blood was collected to determine the amounts of hormones. Real time PCR (qRT-PCR) method was used to detect the related genes expression in hypothalamus, hypophysis, liver and ovary tissues. Ovary tissues slices were made with HE staining and FSHR immunohistochemical staining to observe the morphological structure. The results showed that there were significant differences in ovary structure between high and low double-yolk egg laying ducks, high laying Gaoyou ducks had more developed follicles, and the granulosa cells arranged much closely than low laying Gaoyou ducks. The expression levels of follicle-stimulating hormone (FSH), estradiol (E2) and progesterone (P) were all higher in high laying ducks than those in low laying ducks (P<0.01). qRT-PCR results showed that the expression of FSHR, ERβ and GnRHR in hypothalamus, hypophysis and liver of high laying ducks were significantly higher than those of low-laying ducks (P<0.01), but these genes’ expression had no significant difference in ovary between low and high laying ducks. In conclusion, the ovary structures of high and low double-yolk egg laying ducks have significant differences, the comprehensive regulation of hypothalamus, hypophysis and liver can affect double-yolk egg laying performance of Gaoyou duck.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2017-12-14 基金项目：江苏高校品牌专业建设工程资助项目（PPZY2015A083）；大学生创新创业训练计划项目（201712806003Y）；江苏农牧科技职业学院院级课题（NSF201601、NSF201702）；江苏省六大人才高峰项目（2014-NY-003）；江苏省青蓝工程项目[苏教师(2016)15号]；江苏省农业三新工程项目[SXGC(2017)248] 作者简介：张蕾（1987-），女，江苏常州人，博士研究生，讲师，主要从事动物遗传育种与繁殖研究。(E-mail)2506357124@qq.com 通讯作者：王健，(E-mail)568418583@qq.com
更新日期/Last Update: 2018-05-04