[1]苏文英,纪伟,刘晓梅,等.羊肚菌响应白霉病菌侵染的转录组分析及关键基因克隆[J].江苏农业学报,2026,42(06):1239-1250.[doi:doi:10.3969/j.issn.1000-4440.2026.06.016]
　SU Wenying,JI Wei,LIU Xiaomei,et al.Transcriptome analysis and key gene cloning of Morchella esculenta in response to white mold pathogen infection[J].,2026,42(06):1239-1250.[doi:doi:10.3969/j.issn.1000-4440.2026.06.016]
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羊肚菌响应白霉病菌侵染的转录组分析及关键基因克隆()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年06期
页码:
1239-1250
栏目:
园艺
出版日期:
2026-06-30

文章信息/Info

Title:
Transcriptome analysis and key gene cloning of Morchella esculenta in response to white mold pathogen infection
作者:
苏文英; 纪伟; 刘晓梅; 王一璞; 任立凯
(连云港市农业科学院,江苏连云港222006)
Author(s):
SU Wenying; JI Wei; LIU Xiaomei; WANG Yipu; REN Likai
(Lianyungang Academy of Agricultural Sciences, Lianyungang 222006, China)
关键词:
转录组; 白霉病; 羊肚菌; 基因克隆
Keywords:
transcriptome; white mold; Morchella esculenta; gene cloning
分类号:
S436.46+1
DOI:
doi:10.3969/j.issn.1000-4440.2026.06.016
文献标志码:
A
摘要:
羊肚菌白霉病是由长孢假单隔孢(Pseudodiplospora longispora)侵染引起的羊肚菌常见病害,常导致羊肚菌产量减少和品质降低。为明确长孢假单隔孢对羊肚菌的致病机理,挖掘羊肚菌响应白霉病病原菌侵染的关键基因,本研究通过转录组测序技术对白霉病病原菌侵染前(M1)与侵染3 d(M2)和侵染5 d(M3)的差异表达基因进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析,并对关键基因进行克隆验证。结果表明，侵染前与侵染3 d比较组、侵染前与侵染5 d比较组分别筛选出差异表达基因1 008个和2 955个,羊肚菌中更多基因随侵染时间延长被诱导表达。根据GO分类结果，侵染前与侵染3 d比较组差异表达基因主要富集在DNA整合、DNA代谢过程等生物学功能,侵染前与侵染5 d比较组差异表达基因主要富集在碳水化合物代谢过程、膜部分及氧化还原过程等生物学功能。随着侵染时间延长,羊肚菌抗氧化能力及物质代谢活动显著增强。根据KEGG信号通路富集分析结果，侵染前与侵染5 d比较组差异表达基因在次级代谢产物的生物合成、半乳糖代谢、氨基酸的生物合成、糖酵解/糖异生、丝裂原活化蛋白激酶（MAPK）信号通路-酵母等通路中的富集程度较高,这些通路可能与羊肚菌响应白霉病菌侵染密切相关。长孢假单隔孢侵染能激活羊肚菌高渗甘油-MAPK（HOG-MAPK）信号通路,编码Ste11、Ssk2和Ssk22、HOG1基因表达量上调,细胞通过积累甘油来维持渗透压平衡。糖酵解途径中,磷酸甘油醛脱氢酶基因、磷酸甘油酸激酶基因、磷酸甘油酸变位酶基因、烯醇化酶基因、丙酮酸激酶基因等多个基因表达水平下调,表明白霉病病原菌侵染能抑制丙酮酸生成,进而影响羊肚菌生长发育。克隆获得的MAPK信号通路基因Mshog1 cDNA序列全长1 050 bp,MSHOG1蛋白与真菌HOG1蛋白家族有较高的同源性,且与真菌HOG1同源蛋白都具有“TGY”双重磷酸化位点。本研究结果为羊肚菌抗病育种提供了理论基础。
Abstract:
Morchella esculenta white mold is a common disease caused by Pseudodiplospora longispora, which often leads to the decrease of yield and quality of Morchella esculenta. In order to clarify the pathogenic mechanism of P.longispora on Morchella esculenta and explore the key genes of M. esculenta in response to the infection of pathogen of white mold, this study used transcriptome sequencing technology to perform gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis on differentially expressed genes before (M1) and three days (M2), five days (M3) after infection by pathogen of white mold, and the key gene was cloned and verified. The results showed that 1 008 and 2 955 differentially expressed genes were screened in the comparison group before infection and three days after infection, and the comparison group before infection and five days after infection, respectively. More genes were induced with the extension of infection time. According to the GO classification results, the differentially expressed genes in the comparison group before infection and three days after infection were mainly enriched in biological functions such as DNA integration and DNA metabolism. The differentially expressed genes in the comparison group before infection and five days after infection were mainly enriched in biological functions such as carbohydrate metabolism, membrane part and redox process. As the infection time was prolonged, the antioxidant capacity and metabolic activity of M. esculenta were significantly enhanced. According to the KEGG pathway enrichment analysis results, the differentially expressed genes in the comparison group before infection and five days after infection were highly enriched in the biosynthesis of secondary metabolites, galactose metabolism, biosynthesis of amino acids, glycolysis/gluconeogenesis, mitogen-activated protein kinase (MAPK) signaling pathway-yeast and other pathways. These pathways may be closely related to the response of M. esculenta to P.longispora infection. The infection of P. longispora could activate the high osmolarity glycerol-MAPK (HOG-MAPK) signaling pathway of M. esculenta, and the expression levels of genes encoding Ste11, Ssk2, Ssk22 and HOG1 were up-regulated. The cells maintained osmotic pressure balance by accumulating glycerol. In the glycolysis pathway, the expression levels of glyceraldehyde phosphate dehydrogenase gene, phosphoglycerate kinase gene, phosphoglycerate mutase gene, enolase gene, pyruvate kinase gene and other genes were down-regulated, indicating that the infection of pathogen of white mold could inhibit the production of pyruvate, which in turn affected the growth and development of M. esculenta. The full-length cDNA sequence of MAPK signaling pathway gene Mshog1 obtained by cloning was 1 050 bp. The MSHOG1 protein had high homology with the fungal HOG1 protein family, and had the same “TGY” double phosphorylation site as the fungal HOG1 homologous proteins. The results of this study provide a theoretical basis for disease resistance breeding of M. esculenta.

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备注/Memo

备注/Memo:
收稿日期：2025-09-16基金项目：连云港市财政专项(QNJJ2402)；连云港市“521工程”项目(LYG065212024113)作者简介：苏文英(1992-),女,河南驻马店人,硕士,助理研究员,主要从事食用菌遗传育种工作。(E-mail)18004425758@163.com通讯作者：任立凯,(E-mail)2949823@qq.com
更新日期/Last Update: 2026-07-15