[1]李文静,高鑫博,吴晶慧,等.番茄根特异性基因SlRSP3启动子的鉴定及功能解析[J].江苏农业学报,2026,42(06):1231-1238.[doi:doi:10.3969/j.issn.1000-4440.2026.06.015]
　LI Wenjing,GAO Xinbo,WU Jinghui,et al.Identification and functional analysis of the promoter of the tomato root-specific gene SlRSP3[J].,2026,42(06):1231-1238.[doi:doi:10.3969/j.issn.1000-4440.2026.06.015]
点击复制

番茄根特异性基因SlRSP3启动子的鉴定及功能解析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年06期
页码:
1231-1238
栏目:
园艺
出版日期:
2026-06-30

文章信息/Info

Title:
Identification and functional analysis of the promoter of the tomato root-specific gene SlRSP3
作者:
李文静1; 2; 高鑫博1; 吴晶慧1; 霍明杉1; 赵楠楠1; 雷佳1; 王聪艳1; 张新业1; 朱姝1
(1.廊坊师范学院生命科学学院,河北廊坊065000；2.廊坊市生物样品分析及农残检测重点实验室,河北廊坊065000)
Author(s):
LI Wenjing1; 2; GAO Xinbo1; WU Jinghui1; HUO Mingshan1; ZHAO Nannan1; LEI Jia1; WANG Congyan1; ZHANG Xinye1; ZHU Shu1
(1.College of Life Sciences, Langfang Normal University, Langfang 065000, China;2.Langfang Key Laboratory of Biological Sample Analysis and Pesticide Residue Detection, Langfang 065000, China)
关键词:
番茄; 根特异性基因启动子; 顺式作用元件
Keywords:
tomato; root-specific gene promoter; cis-acting elements
分类号:
Q75；Q78
DOI:
doi:10.3969/j.issn.1000-4440.2026.06.015
文献标志码:
A
摘要:
根是植物重要的营养器官,其生长发育直接影响作物抗逆能力与产量形成,因此了解根特异性表达基因及其启动子对作物改良具有重要意义。本研究基于番茄不同组织部位(根、茎、叶、花、果实)的转录组数据,筛选出根中优势表达基因Solyc03g096420,命名为SlRSP3。利用荧光定量PCR分析SlRSP3基因的表达模式,克隆SlRSP3基因的启动子,根据顺式作用元件的分布位置采用5′端缺失法对启动子进行分析,并通过同源重组法构建SlRSP3基因的全长及截短启动子融合β-葡萄糖醛酸苷酶报告基因(GUS)的表达载体,转化拟南芥进行组织表达模式分析。荧光定量PCR分析结果表明,SlRSP3基因在番茄根部特异表达。顺式作用元件分析结果表明,SlRSP3基因启动子内部含有根部特异性表达元件；GUS组织化学染色结果表明,2 000 bp和1 300 bp SlRSP3基因启动子特异性在拟南芥根部表达,而700 bp及300 bp SlRSP3基因启动子呈组成型表达。综上,本研究中SlRSP3基因启动子为根特异性基因启动子,1 300 bp SlRSP3基因启动子可以取代2 000 bp 启动子行使功能,在番茄抗逆遗传改良中具有重要意义。
Abstract:
Roots are important vegetative organs, and their growth and development directly affect crop stress resistance and yield formation. Therefore, understanding root-specific expressed genes and their promoters is of great significance for crop improvement. Based on transcriptome data from different tissues (root, stem, leaf， flower and fruit) of tomato, a root-predominant gene, Solyc03g096420, was identified and designated as SlRSP3. The expression pattern of the SlRSP3 gene was analyzed by quantitative real-time PCR (qRT-PCR). The promoter of the SlRSP3 gene was cloned, and a 5′-deletion analysis was performed based on the distribution of cis-acting elements. Subsequently, expression vectors carrying the full-length and truncated SlRSP3 promoters fused with the β-glucuronidase (GUS) reporter gene were constructed via homologous recombination and transformed into Arabidopsis thaliana for tissue-specific expression pattern analysis. The quantitative real-time PCR (qRT-PCR) results showed that the SlRSP3 gene was specifically expressed in tomato roots. Cis-acting element analysis revealed that the promoter of the SlRSP3 gene contained root-specific expression elements. GUS histochemical staining results revealed that the 2 000 bp and 1 300 bp SlRSP3 gene promoter fragments drove specific expression in Arabidopsis roots, whereas the 700 bp and 300 bp SlRSP3 gene promoter fragments exhibited constitutive expression. In summary, the SlRSP3 gene promoter identified in this study is a root-specific promoter, and the 1 300 bp SlRSP3 promoter can replace the 2 000 bp promoter in function, which is of great significance for the genetic improvement of stress tolerance in tomato.

参考文献/References:

[1]CAI T C, CHEN H, YAN L M, et al. The root-specific NtR12 promoter-based expression of RIP increased the resistance against bacterial wilt disease in tobacco[J]. Molecular Biology Reports,2022,49(11):503-514.
[2]闫强,薛冬,胡亚群,等. 大豆根特异性GmPR1-9启动子的鉴定及其在根腐病抗性中的应用[J]. 中国农业科学,2022,55(20):3885-3896.
[3]BOROUJENI N A, KHATOUNI S B, MOTAMEDI M J, et al. Root-preferential expression of newcastle virus glycoproteins driven by NtREL1 promoter in tobacco hairy roots and evaluation of oral delivery in mice[J]. Transgenic Research,2022,31(2):201-213.
[4]ZHANG C, PAN S F, CHEN H, et al. Characterization of NtREL1,a novel root-specific gene from tobacco,and upstream promoter activity analysis in homologous and heterologous hosts[J]. Plant Cell Reports,2016,35(4):757-769.
[5]CHEN L, JIANG B J, WU C X, et al. GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots[J]. BMC Plant Biology,2014,14:245.
[6]CHEN L, JIANG B J, WU C X, et al. The characterization of GmTIP,a root-specific gene from soybean,and the expression analysis of its promoter[J]. Plant Cell,Tissue and Organ Culture (PCTOC),2015,121(2):259-274.
[7]李文静,李甜饴,乔洁,等. 番茄SlTIP-1基因的克隆、表达及启动子活性分析[J]. 植物生理学报,2020,56(6):1221-1230.
[8]冯婵莹,李涛,黎振兴,等. 番茄根特异基因的表达分析[J]. 植物生理学报,2015,51(6):921-934.
[9]VAUGHAN S P, JAMES D J, LINDSEY K, et al. Characterization of FaRB7,a near root-specific gene from strawberry (Fragariaxananassa Duch.) and promoter activity analysis in homologous and heterologous hosts[J]. Journal of Experimental Botany,2006,57(14):3901-3910.
[10]XUN H W, ZHANG X, YU J M, et al. Analysis of expression characteristics of soybean leaf and root tissue-specific promoters in Arabidopsis and soybean[J]. Transgenic Research,2021,30(6):799-810.
[11]LIU J J, EKRAMODDOULLAH A K. Root-specific expression of a western white pine PR10 gene is mediated by different promoter region in transgenic tobacco[J]. Plant Molecular Biology,2003,52(1):103-120.
[12]王佳音,张云逸,薛婷婷,等. 番茄磷酸盐转运蛋白SlPT2基因启动子克隆及 功能分析[J]. 河南农业科学,2024,53(6):120-127.
[13]李文静,张新业,张智研,等. 番茄SlNRT2基因家族鉴定、启动子克隆及表达分析[J]. 植物生理学报,2024,60(1):97-107.
[14]JENNY P, SAKURE A A, YADAV A, et al. Molecular cloning and characterisation of root-specific SlREO promoter of the Indian tomato (Solanum lycopersicum L.) cultivar[J]. Functional Plant Biology,2024,51:24063.
[15]MOISSEYEV G, PARK K, CUI A, et al. RGPDB database of root-associated genes and promoters in maize,soybean,and Sorghum[J]. Database,2020,2020:38.
[16]GAO S, CHAI Y, ZHOU X Y, et al. Mining of root-specific expression genes and their core cis-regulatory elements in plants[J]. International Journal of Molecular Sciences,2025,26(4):1720.
[17]LI Y, CHEN Y, ZHOU L, et al. MicroTom metabolic network:rewiring tomato metabolic regulatory network throughout the growth cycle[J]. Molecular Plant,2020,13(8):1203-1218.
[18]SONG X G, MENG X B, GUO H Y, et al. Targeting a gene regulatory element enhances rice grain yield by decoupling panicle number and size[J]. Nature Biotechnology,2022,40:1403-1411.
[19]LESCOT M, DHAIS P, THIJS G, et al. A database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences[J]. Nucleic Acids Research,2002,30(4):325-327.
[20]HIGO K, UGAWA Y, IWAMOTO M, et al. Plant cis-acting regulatory DNA elements (PLACE) database:1999[J]. Nucleic Acids Research,1999,27(1):297-300.
[21]KHANDAL H, GUPTA S K, DWIVEDI V, et al. Root-specific expression of chickpea cytokinin oxidase/dehydrogenase 6 leads to enhanced root growth,drought tolerance and yield without compromising nodulation[J]. Plant Biotechnology Journal,2020,18(11):2225-2240.
[22]HUANG L Y, ZHANG F, QIN Q, et al. Identification and validation of root-specific promoters in rice[J]. Journal of Integrative Agriculture,2015,14(1):1-10.
[23]JEONG H J, JUNG K H. Rice tissue-specific promoters and condition-dependent promoters for effective translational application[J]. Journal of Integrative Plant Biology,2015,57(11):913-924.
[24]LI Y, LIU X Q, CHEN R M, et al. Genome-scale mining of root-preferential genes from maize and characterization of their promoter activity[J]. BMC Plant Biology,2019,19(1):584.
[25]薛冬,闫强,胡亚群,等. 大豆根系特异表达基因全基因组水平挖掘及其启动子活性鉴定[J]. 植物生理学报,2023,59(1):55-66.
[26]YU L L, ZHANG H, GUAN R X, et al. Genome-wide tissue-specific genes identification for novel tissue-specific promoters discovery in soybean[J]. Genes,2023,14(6):1150.
[27]李文静,孙艳香. 水稻谷蛋白基因GluC启动子的克隆及表达分析[J]. 植物研究,2018,38(6):921-930.
[28]DONG Q, JIANG H Y, XU Q Q, et al. Cloning and characterization of a multifunctional promoter from maize (Zea mays L.)[J]. Applied Biochemistry and Biotechnology,2015,175(3):1344-1357.
[29]TAKAIWA F. Influence on accumulation levels and subcellular localization of prolamins by fusion with the functional peptide in transgenic rice seeds[J]. Molecular Biotechnology,2023,65(11):1869-1886.

相似文献/References:

[1]赵丽萍,李永灿,赵统敏,等.灰霉菌毒素诱导番茄抗性突变体及相关防御酶活性[J].江苏农业学报,2016,(03):631.[doi:10.3969/j.issn.1000-4440.2016.03.023]
　ZHAO Li-ping,LI Yong-can,ZHAO Tong-min,et al.Resistant mutant induced by toxin of Botrytis cinerea in tomato and the activities of related defensive enzymes[J].,2016,(06):631.[doi:10.3969/j.issn.1000-4440.2016.03.023]
[2]李亚茹,王银磊,赵丽萍,等.番茄ty-5和 Mi-1基因多重 PCR 体系的建立[J].江苏农业学报,2016,(04):869.[doi:10.3969/j.issn.100-4440.2016.04.024]
　LI Ya-ru,WANG Yin-lei,ZHAO Li-ping,et al.Identification of ty-5 gene and Mi-1 gene by multiplex PCR in tomato[J].,2016,(06):869.[doi:10.3969/j.issn.100-4440.2016.04.024]
[3]吕敏,苏建坤,白和盛,等.桃蚜取食和机械损伤对番茄和辣椒 PAL、LOX 和 PPO 活性的诱导作用[J].江苏农业学报,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
　Lv?Min,SU Jian-kun,BAI He-sheng,et al.The activities of PAL, LOX and PPO in tomato and pepper plants induced by aphid herbivory and mechanical damage[J].,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
[4]姜静,王银磊,赵丽萍,等.番茄qRT-PCR内参基因的筛选[J].江苏农业学报,2017,(02):389.[doi:doi:10.3969/j.issn.1000-4440.2017.02.024]
　JIANG Jing,WANG Yin-lei,ZHAO Li-ping,et al.Selection of tomato reference genes for qRT-PCR[J].,2017,(06):389.[doi:doi:10.3969/j.issn.1000-4440.2017.02.024]
[5]罗伟君,唐琳,周佳丽,等.纳米锌肥对番茄果实锌含量与品质的强化[J].江苏农业学报,2016,(01):184.[doi:10.3969/j.issn.1000-4440.2016.01.028 ]
　LUO Wei-jun,TANG Lin,ZHOU Jia-li,et al.Improvement of zinc concentration and quality of tomato fruit by nano zinc fertilizer[J].,2016,(06):184.[doi:10.3969/j.issn.1000-4440.2016.01.028 ]
[6]乔俊卿,陈志谊,梁雪杰,等.枯草芽孢杆菌Bs916在番茄根部的定殖[J].江苏农业学报,2015,(06):1278.[doi:doi:10.3969/j.issn.1000-4440.2015.06.013]
　QIAO Jun-qing,CHEN Zhi-yi,LIANG Xue-jie,et al.Colonization of Bacillus subtilis Bs916 on tomato root[J].,2015,(06):1278.[doi:doi:10.3969/j.issn.1000-4440.2015.06.013]
[7]翟亚明,魏丽萍,杨倩.不同调控方式对设施盐渍化土壤特性和番茄产量及品质的影响[J].江苏农业学报,2015,(04):871.[doi:10.3969/j.issn.1000-4440.2015.04.025]
　ZHAI Ya-ming,WEI Li-ping,YANG Qian.Effects of regulatory measures on characters of greenhouse saline soil and tomato yield and quality[J].,2015,(06):871.[doi:10.3969/j.issn.1000-4440.2015.04.025]
[8]杨玛丽,赵统敏,余文贵,等.转基因 RNAi 技术在番茄研究中的应用[J].江苏农业学报,2015,(01):217.[doi:10.3969/j.issn.1000-4440.2015.01.034]
　YANG Ma-li,ZHAO Tong-min,YU Wen-gui,et al.Progress in RNA interference technique applied in tomato[J].,2015,(06):217.[doi:10.3969/j.issn.1000-4440.2015.01.034]
[9]唐玉新,曲萍,陆岱鹏,等.适合机械化移栽的番茄穴盘育苗基质配方筛选[J].江苏农业学报,2017,(06):1342.[doi:doi:10.3969/j.issn.1000-4440.2017.06.021]
　TANG Yu-xin,QU Ping,LU Dai-peng,et al.Screening of tomato plug seedling substrates proportion suitable for mechanized transplanting[J].,2017,(06):1342.[doi:doi:10.3969/j.issn.1000-4440.2017.06.021]
[10]姜静,王银磊,李亚茹,等.江苏省及其他地区番茄黄化曲叶病毒的分子鉴定及序列分析[J].江苏农业学报,2018,(01):238.[doi:doi:10.3969/j.issn.1000-4440.2018.01.035]
　JIANG Jing,WANG Yin-lei,LI Ya-ru,et al.Molecular identification and sequence analysis of tomato yellow leaf curl virus in Jiangsu province and its peripheral area[J].,2018,(06):238.[doi:doi:10.3969/j.issn.1000-4440.2018.01.035]

备注/Memo

备注/Memo:
收稿日期：2025-06-30基金项目：河北省省属高等学校基本科研业务费研究项目(JYQ202101)作者简介：李文静(1982-),女,山东德州人,博士,副教授,主要从事分子生物学研究。(E-mail)liwenjing@lfnu.edu.cn通讯作者：朱姝,(E-mail)zhushu@lfnu.edu.cn;张新业,(E-mail)zhigancao@126.com
更新日期/Last Update: 2026-07-15