[1]姚凯文,王成浩,逯佳琪,等.基于微创组织取样检测的番茄枯萎病预警技术[J].江苏农业学报,2026,42(06):1148-1157.[doi:doi:10.3969/j.issn.1000-4440.2026.06.007]
　YAO Kaiwen,WANG Chenghao,LU Jiaqi,et al.Tomato wilt disease early warning technology based on minimally invasive tissue sampling and detection[J].,2026,42(06):1148-1157.[doi:doi:10.3969/j.issn.1000-4440.2026.06.007]
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基于微创组织取样检测的番茄枯萎病预警技术()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年06期
页码:
1148-1157
栏目:
植物保护
出版日期:
2026-06-30

文章信息/Info

Title:
Tomato wilt disease early warning technology based on minimally invasive tissue sampling and detection
作者:
姚凯文1; 2; 王成浩2; 逯佳琪2; 褚姝频3; 卢鹏3; 乐秀虎1; 魏利辉2; 邓晟2
(1.河北工程大学园林与生态工程学院,河北邯郸056038；2.江苏省农业科学院植物保护研究所,江苏南京210014；3.江苏省植物保护植物检疫站,江苏南京210036)
Author(s):
YAO Kaiwen1; 2; WANG Chenghao2; LU Jiaqi2; CHU Shupin3; LU Peng3; LE Xiuhu1; WEI Lihui2; DENG Sheng2
(1.School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, China;2.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.Plant Protection and Quarantine Station of Jiangsu Province, Nanjing 210036, China)
关键词:
番茄枯萎病; 尖孢镰刀菌; 微创穿刺取样; 环介导核酸等温扩增（LAMP）; 重组酶介导等温扩增(RAA)
Keywords:
tomato wilt; Fusarium oxysporum; minimally invasive puncture sampling; loop-mediated isothermal amplification （LAMP）; recombinase-aided amplification (RAA)
分类号:
S641.2
DOI:
doi:10.3969/j.issn.1000-4440.2026.06.007
文献标志码:
A
摘要:
枯萎病是设施番茄生产中常见的土传性病害,传统植物保护策略以预防为主,病原菌侵染植株显症后,目前无有效防治方法。本研究创新性采用微创穿刺取样技术,结合环介导核酸等温扩增(LAMP)和重组酶介导核酸等温扩增(RAA)检测技术,构建了番茄枯萎病精准检测及防治技术体系。首先,通过无菌注射器针头在番茄茎基部穿刺获取微量维管束组织,取样后取样植株与对照植株相比,生长无显著差异；其次,在室内人工接种条件下,通过微创组织取样方法,结合LAMP和RAA检测技术,可在典型症状出现前检测到植株维管束中靶标病原菌,带菌植株阳性检出率为47.6%,为病害早期治疗提供了重要的时间窗口；在此窗口期内,及时采用三氟吡啶胺、贝莱斯芽孢杆菌JSBV63进行灌根处理,两次施药后防治效果可达到90.00%以上，甚至还有增绿和促进生长的效果。该微创取样+LAMP和RAA检测+药剂处理,实现了从病原菌侵染早期诊断到病害防治的一整套枯萎病防治技术体系。本研究结果丰富了维管束病害的预防与控制技术措施,为后续作物维管束病害预防、预警和防治提供了理论依据和技术支撑。
Abstract:
Fusarium wilt is a common soil-borne disease in protected tomato production. Traditional plant protection strategies focus primarily on prevention, and there are currently no effective curative measures once plants are infected by pathogens and show consequent symptoms. In this study, we established a precision control technology system for tomato Fusarium wilt by employing minimal-wound puncture sampling combined with loop-mediated isothermal amplification (LAMP) and recombinase-aided amplification (RAA) detection. Firstly, micro-samples of vascular bundle tissue were obtained by puncturing the stem base of tomato plants using sterile syringe needles. Compared with non-sampled plants, sampled plants showed no significant differences in growth. Secondly, under controlled artificial inoculation conditions, using the minimally invasive tissue sampling method combined with LAMP and RAA detection technologies, the target pathogen in the plant vascular tissue could be detected before the appearance of typical symptoms. The positive detection rate of infected plants was 47.6%, providing a crucial time window for early disease treatment. Within this window, timely root drenching treatments with cyclobutrifluram and Bacillus velezensis strain JSBV63 achieved a control efficacy of over 90.00% after two applications, and even exhibited greening and growth-promoting effects. The combination of minimally invasive sampling, LAMP and RAA detection, and chemical/biological treatment achieved a complete technical system for Fusarium wilt control, spanning from early pathogen infection diagnosis to disease treatment. This study enriches the control strategies for vascular diseases and provides a theoretical foundation and technical support for the future prevention, early warning, and treatment of tomato Fusarium wilt and other crop vascular diseases.

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备注/Memo

备注/Memo:
收稿日期:2025-08-13基金项目:财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-24-C-01)；江苏省农业科技自主创新基金项目[CX(23)1033]；邯郸市科学技术研究与发展计划项目(21422012329)作者简介:姚凯文(2000-),男,山东枣庄人,硕士研究生,研究方向为土传病害。(E-mail)1977814285@qq.com。王成浩为共同第一作者。通讯作者:乐秀虎,(E-mail)lexiuhu@163.com；邓晟,(E-mail)dengsheng@jaas.ac.cn
更新日期/Last Update: 2026-07-15