[1]赵冬兰,肖世卓,周志林,等.甘薯抗旱QTL的鉴定与候选基因分析[J].江苏农业学报,2026,42(03):485-491.[doi:doi:10.3969/j.issn.1000-4440.2026.03.006]
 ZHAO Donglan,XIAO Shizhuo,ZHOU Zhilin,et al.Identification of drought-resistant QTLs and analysis of candidate genes in sweetpotato[J].,2026,42(03):485-491.[doi:doi:10.3969/j.issn.1000-4440.2026.03.006]
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甘薯抗旱QTL的鉴定与候选基因分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
485-491
栏目:
遗传育种·生理生化
出版日期:
2026-03-31

文章信息/Info

Title:
Identification of drought-resistant QTLs and analysis of candidate genes in sweetpotato
作者:
赵冬兰肖世卓周志林赵凌霄戴习彬张安王洁袁蕊李青莲王珧曹清河
(江苏徐淮地区徐州农业科学研究所,江苏徐州221131)
Author(s):
ZHAO DonglanXIAO ShizhuoZHOU ZhilinZHAO LingxiaoDAI XibinZHANG AnWANG JieYUAN RuiLI QinglianWANG YaoCAO Qinghe
(Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Xuzhou 221131, China)
关键词:
甘薯抗旱性数量性状位点候选基因
Keywords:
sweetpotatodrought resistancequantitative trait locuscandidate gene
分类号:
S531
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.006
文献标志码:
A
摘要:
干旱是限制作物生产最严重的非生物胁迫之一,解析抗旱遗传基础、发掘关键基因与分子标记对甘薯抗旱品种选育具有重要意义。本研究以包含212个子代的F1群体为试验材料,在新疆干旱环境下进行抗旱表型鉴定。基于甘薯单核苷酸多态性(SNP)遗传图谱,采用复合区间作图法(CIM)鉴定到1个与甘薯抗旱性相关的数量性状基因座(QTL)qDR04-1,其表型贡献率为11.1%。进一步对qDR04-1置信区间进行基因预测与功能注释,筛选出10个抗旱候选基因,功能涉及离子转运、脂质代谢、糖基化修饰、氧化应激响应及WRKY转录调控等逆境适应通路。本研究首次在甘薯中定位到抗旱QTL并系统挖掘了其候选基因,为抗旱分子机制解析与分子育种提供了理论依据。
Abstract:
Drought is one of the most severe abiotic stresses limiting crop production. Dissecting the genetic basis of drought resistance and identifying key genes and molecular markers are of great significance for breeding drought-resistant sweetpotato varieties. In this study, an F1 population consisting of 212 progenies was used as experimental material for the identification of drought-resistant phenotypes under the arid environment in Xinjiang. Based on the sweetpotato single nucleotide polymorphism (SNP) genetic map, the composite interval mapping (CIM) method was adopted to detect one quantitative trait locus (QTL) qDR04-1 associated with drought resistance in sweetpotato, with a phenotypic contribution rate of 11.1%. Further gene prediction and functional annotation were performed on the confidence interval of qDR04-1, and ten drought-resistant candidate genes were screened out, whose functions were involved in stress adaptation pathways such as ion transport, lipid metabolism, glycosylation modification, oxidative stress response and WRKY transcriptional regulation. This study is the first to map the drought-resistant QTLs in sweetpotato and systematically identify its candidate genes, which provides a theoretical basis for the dissection of drought-resistant molecular mechanisms and molecular breeding.

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备注/Memo

备注/Memo:
收稿日期:2025-07-09基金项目:国家重点研发计划项目(2023YFD1202703);国家现代农业产业技术体系项目(CARS-10-GW01);江苏省重点研发计划项目(BE2021384);国家科技资源共享服务平台项目(NCGRC-43)作者简介:赵冬兰(1972-),女,陕西泾阳人,硕士,研究员,主要从事甘薯种质资源研究。(E-mail)19991006@jaas.ac.cn
更新日期/Last Update: 2026-04-17