[1]孙计平,王亚乐,李雪君,等.基于转录组测序筛选烟草响应疫霉侵染的候选基因[J].江苏农业学报,2025,(03):457-467.[doi:doi:10.3969/j.issn.1000-4440.2025.03.004]
　SUN Jiping,WANG Yale,LI Xuejun,et al.Screening of candidate genes in response of tobacco to Phytophthora nicotianae infection based on transcriptome sequencing[J].,2025,(03):457-467.[doi:doi:10.3969/j.issn.1000-4440.2025.03.004]
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基于转录组测序筛选烟草响应疫霉侵染的候选基因()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年03期
页码:
457-467
栏目:
遗传育种·生理生化
出版日期:
2025-03-31

文章信息/Info

Title:
Screening of candidate genes in response of tobacco to Phytophthora nicotianae infection based on transcriptome sequencing
作者:
孙计平; 王亚乐; 李雪君; 平文丽; 孙焕
(河南省农业科学院烟草研究所,河南许昌461000)
Author(s):
SUN Jiping; WANG Yale; LI Xuejun; PING Wenli; SUN Huan
(Tobacco Research Institute, Henan Academy of Agricultural Sciences, Xuchang 461000, China)
关键词:
转录组; 疫霉; 河洛1号; 烟草
Keywords:
transcriptome; Phytophthora nicotianae; Heluo No.1; tobacco
分类号:
S572；S435.72
DOI:
doi:10.3969/j.issn.1000-4440.2025.03.004
文献标志码:
A
摘要:
疫霉属真菌是主要的植物病原菌,会对很多作物造成毁灭性伤害,为挖掘烟草响应疫霉侵染的候选基因,用烟草疫霉菌侵染河洛1号幼苗,通过转录组测序技术,对疫霉菌侵染前与侵染后24 h和48 h的差异表达基因(DEG)进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析,并利用实时定量聚合酶链反应(qRT-PCR)技术验证。结果表明,疫霉菌侵染后24 h和48 h分别筛选出DEG 4 563个和14 053个,共同变化的基因3 623个,其中1 502个上调差异表达基因被GO基因组注释,主要富集在谷胱甘肽代谢过程和蛋白质磷酸化生物学过程；787个下调差异表达基因被GO基因组注释,主要富集在转录调控。KEGG富集分析结果显示,差异表达基因主要富集在植物-丝裂原活化蛋白激酶信号通路、植物激素信号转导通路和植物-病原菌互作通路。植物-病原菌互作通路中FLS2上调促进MEKK1磷酸化,诱导蛋白激酶MPK4，引起WRKY12持续下调表达,WRKY9、WRKY24、WRKY26、WRKY31、WRKY33、WRKY40持续上调表达；诱导蛋白激酶MPK3/6表达,引起WRKY11、WRKY15、WRKY17和WRKY41上调表达,导致PR-1基因持续下调表达,河洛1号植株感病。
Abstract:
Phytophthora fungi are important plant pathogens that cause devastating damage to many crops. To identify candidate genes in tobacco that respond to Phytophthora nicotianae infection, seedlings of Heluo No.1 were infected with Phytophthora nicotianae. Differential expression genes (DEGs) before infection and 24 h, 48 h after infection were analyzed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, based on transcriptome sequencing. The results were verified by quantitative real-time polymerase chain reaction (qRT-PCR). The results indicated that 4 563 DEGs and 14 053 DEGs were identified at 24 h and 48 h post-infection, respectively, with 3 623 genes showing common changes. Among them, 1 502 up-regulated DEGs were annotated by GO genome, mainly enriched in glutathione metabolism process and protein phosphorylation biological process. A total of 787 down-regulated DEGs were annotated by GO genome, mainly enriched in transcriptional regulation. KEGG enrichment analysis showed that DEGs were primarily enriched in the plant-mitogen activated protein kinase signaling pathway, plant hormone signal transduction pathway and plant-pathogen interaction pathway. In the plant-pathogen interaction pathway, the up-regulation of FLS2 promoted the phosphorylation of MEKK1, which induced the expression of protein kinase MPK4, caused the continuous down-regulation of WRKY12 and the continuous up-regulation of WRKY9, WRKY24, WRKY26, WRKY31, WRKY33 and WRKY40. Besides, the expression of protein kinases MPK3/6 was induced, leading to the up-regulation of WRKY11, WRKY15, WRKY17 and WRKY41, and the continuous down-regulation of the PR-1 gene. As a result, Heluo No.1 plants were more susceptible to Phytophthora nicotianae infection.

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备注/Memo

备注/Memo:
收稿日期:2024-05-22基金项目:河南省烟草公司洛阳市公司科技项目(2022410300200053)；河南省农业科学院基础性科研工作项目(2024JC09)作者简介:孙计平(1978-),女,河北玉田人,博士,副研究员,研究方向为烟草遗传育种。(E-mail)sunjiping2002@126.com
更新日期/Last Update: 2025-04-27