[1]杨建帮,姚文孔,杨丽,等.枸杞中的褪黑素含量及其积累特征[J].江苏农业学报,2025,(01):169-174.[doi:doi:10.3969/j.issn.1000-4440.2025.01.019]
　YANG Jianbang,YAO Wenkong,YANG Li,et al.The content and accumulation characteristics of melatonin in Lycium barbarum[J].,2025,(01):169-174.[doi:doi:10.3969/j.issn.1000-4440.2025.01.019]
点击复制

枸杞中的褪黑素含量及其积累特征()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年01期
页码:
169-174
栏目:
园艺
出版日期:
2025-01-31

文章信息/Info

Title:
The content and accumulation characteristics of melatonin in Lycium barbarum
作者:
杨建帮1; 姚文孔1; 2; 杨丽1; 冯美1; 2
(1.宁夏大学葡萄酒与园艺学院,宁夏银川750021；2.宁夏现代设施园艺工程技术研究中心,宁夏银川750021)
Author(s):
YANG Jianbang1; YAO Wenkong1; 2; YANG Li1; FENG Mei1; 2
(1.School of Enology and Horticulture, Ningxia University, Yinchuan 750021, China;2.Ningxia Modern Facility Horticulture Engineering Technology Research Center, Yinchuan 750021, China)
关键词:
枸杞; 褪黑素; 积累特征; 盐胁迫; 果实发育
Keywords:
Lycium barbarum L.; melatonin; accumulating characteristics; salt stress; fruit development
分类号:
S663.9
DOI:
doi:10.3969/j.issn.1000-4440.2025.01.019
文献标志码:
A
摘要:
为探究枸杞中的褪黑素含量及其积累特征,使用宁夏6个枸杞材料,通过高效液相色谱法测定褪黑素含量,分析枸杞不同器官、果实不同发育期、不同成熟期、不同材料、不同立地条件下的褪黑素含量,并分析土壤理化因子与枸杞不同器官中褪黑素含量间的关系。结果表明,枸杞不同器官中的褪黑素含量存在一定差异,其中褪黑素含量大小通常遵循花>叶>茎>根。在春果青果期与变色期的果实中检测到褪黑素,在黄熟期与红熟期的春果果实中未检测到褪黑素；在秋果不同果实发育时期的果实中均检测到褪黑素,在青果期果实中的褪黑素含量最高,之后随着果实的发育,果实中的褪黑素含量逐渐下降,到果实成熟时又有所上升。宁杞菜1号各器官中的褪黑素含量显著高于宁杞1号,在不同材料的枸杞叶片中,褪黑素含量排序为宁杞菜1号>宁杞7号>黄果枸杞>黑果枸杞>宁杞10号>宁杞1号。在盐分含量高的立地条件下,枸杞各器官中的褪黑素含量显著增加。以上结果表明,枸杞中含有丰富的褪黑素,对于同一枸杞材料而言,各器官中褪黑素含量的排序为花>叶>茎>根,褪黑素含量在果实发育过程中的青果期、变色期较高,秋果中各器官的褪黑素含量大于春果,枸杞在高盐环境中有利于体内褪黑素的积累。
Abstract:
To explore the content and accumulation characteristics of melatonin in Lycium barbarum, six Lycium barbarum materials in Ningxia were used, and the content of melatonin was determined by high performance liquid chromatography. The melatonin content in different organs of Lycium barbarum, at different developmental stages of the fruits, at different maturity stages, in different materials and under different site conditions was analyzed. Moreover, the relationship between soil physical and chemical factors and the melatonin content in different organs of Lycium barbarum was analyzed. The results showed that there were some differences in the content of melatonin in different organs of Lycium barbarum, and the content of melatonin usually followed the order of flower > leaf > stem > root. Melatonin was detected in spring fruits at green fruit stage and discoloration stage, and melatonin was not detected in fruits at yellow ripening stage and red ripening stage. Melatonin was detected in autumn fruits at different developmental stages. Melatonin content was highest in fruits at the green fruit stage. Subsequently, as the fruits developed, the melatonin content in the fruits gradually declined, and then increased again when the fruits ripened. The content of melatonin in each organ of Ningqicai No.1 was significantly higher than that of Ningqi No.1. In the leaves of different materials, the melatonin content followed the order of Ningqicai No.1 > Ningqi No.7 > Huangguo wolfberry > Heiguo wolfberry > Ningqi No.10 > Ningqi No.1. Under high-salt conditions, the content of melatonin in each organ of Lycium barbarum increased significantly. The above results indicated that Lycium barbarum was rich in melatonin. For the same Lycium barbarum material, the order of melatonin content in each organ was flower > leaf > stem > root. Melatonin content was higher in the green fruit period and discoloration period during fruit development. The melatonin content of each organ in autumn fruits was higher than that in spring fruits. Lycium barbarum in high salt environment was conducive to the accumulation of melatonin.

参考文献/References:

[1]LERNER A B, CASE J D, TAKAHASHI Y, et al. Isolation of melatonin,the pineal gland factor that lightens melanocytes[J]. Journal of the American Chemical Society,1958,80(10):2587.
[2]ARNAO M B, HERNNDEZ-RUIZ J. Melatonin in flowering,fruit set and fruit ripening[J]. Plant Reproduction,2020,33(2):77-87.
[3]REITER R J, MANCHESTER L C, TAN D X. Melatonin in walnuts:influence on levels of melatonin and total antioxidant capacity of blood[J]. Nutrition,2005,21(9):920-924.
[4]魏雪松,王海洋,孙智轩,等. 宁夏枸杞化学成分及其药理活性研究进展[J]. 中成药,2018,40(11):2513-2520.
[5]胡冬梅. 宁夏大力发展“六特”产业[N]. 中国食品安全报, 2022-07-26(A03).
[6]CHEN G F, HUO Y S, TAN D X, et al. Melatonin in Chinese medicinal herbs[J]. Life Sciences,2003,73(1):19-26.
[7]赵建芬,杨海贵,李静仪. HPLC-UV法检测芦荟、枸杞叶和桑叶中的褪黑素[J]. 食品工业,2015,36(2):266-270.
[8]刘定. 芍药褪黑素积累规律及其合成基因TDC功能的初步研究[D]. 扬州:扬州大学,2017.
[9]王旭,任琳,邹晓莉,等. 同时测定保健食品中褪黑素和维生素B6的高效液相色谱方法研究[J]. 现代预防医学,2012,39(23):6245-6247,6250.
[10]鲍士旦. 土壤农化分析[M]. 3版. 北京:中国农业出版社,2000:189-193.
[11]王蕊,杨小龙,须晖,等. 高等植物褪黑素的合成和代谢研究进展[J]. 植物生理学报,2016,52(5):615-627.
[12]刘定,赵大球,陆春良,等. 采用高效液相色谱-串联质谱法检测芍药中褪黑素含量[J]. 分子植物育种,2017,15(4):1527-1534.
[13]ERLAND L A E, CHATTOPADHYAY A, JONES A M P, et al. Melatonin in plants and plant culture systems:variability,stability and efficient quantification[J]. Frontiers in Plant Science,2016,7:1721.
[14]BYEON Y, PARK S, KIM Y S, et al. Light-regulated melatonin biosynthesis in rice during the senescence process in detached leaves[J]. Journal of Pineal Research,2012,53(1):107-111.
[15]吴艳迪. 葡萄褪黑素含量变化及其合成基因SNAT原核表达分析[D]. 北京:中国农业科学院,2018.
[16]KORKMAZ A, DEGER , CUCI Y. Profiling the melatonin content in organs of the pepper plant during different growth stages[J]. Scientia Horticulturae,2014,172:242-247.
[17]郑莎. 桑树褪黑素及其异构体生物合成机制研究[D]. 重庆:西南大学,2021.
[18]卞凤娥. 葡萄中的褪黑素及外源褪黑素的抗逆功能[D]. 泰安:山东农业大学,2017.
[19]STRTZ M, CEREZO A B, CANTOS-VILLAR E, et al. Determination of the melatonin content of different varieties of tomatoes (Lycopersicon esculentum) and strawberries (Fragaria ananassa)[J]. Food Chemistry,2011,127(3):1329-1334.
[20]RIGA P, MEDINA S, GARCA-FLORES L A, et al. Melatonin content of pepper and tomato fruits:effects of cultivar and solar radiation[J]. Food Chemistry,2014,156:347-352.
[21]WANG C, YIN L Y, SHI X Y, et al. Effect of cultivar,temperature,and environmental conditions on the dynamic change of melatonin in mulberry fruit development and wine fermentation[J]. Journal of Food Science,2016,81(4):M958-M967.
[22]王博美. 外施褪黑素对低氮下小麦生长的调控效应及其机理[D]. 杨凌:西北农林科技大学,2018.
[23]徐宁莉. 非生物胁迫对青稞褪黑素等功能成分影响及代谢组学分析[D]. 芜湖:安徽工程大学,2023.
[24]李帜奇,袁月,苗荣庆,等. 盐胁迫盐芥和拟南芥褪黑素含量及合成相关基因表达模式分析[J]. 生物技术通报,2023,39(5):142-151.
[25]吴鹏, 吕剑, 郁继华, 等. 褪黑素对盐碱复合胁迫下黄瓜幼苗光合特性和渗透调节物质含量的影响[J]. 应用生态学报, 2022,33(7):1901-1910.

相似文献/References:

[1]王淑娟,刘文举,庞训胜,等.褪黑素对牛卵母细胞体外成熟及孤雌胚胎发育的影响[J].江苏农业学报,2017,(02):361.[doi:doi:10.3969/j.issn.1000-4440.2017.02.019]
　WANG Shu-juan,LIU Wen-ju,PANG Xun-sheng,et al.Influence of melatonin on oocyte in vitro maturation and parthenogenetic embryo development in bovine[J].,2017,(01):361.[doi:doi:10.3969/j.issn.1000-4440.2017.02.019]
[2]赵建华,李浩霞,尹跃,等.枸杞酸性转化酶基因的克隆与表达[J].江苏农业学报,2015,(05):1140.[doi:doi:10.3969/j.issn.1000-4440.2015.05.031]
　ZHAO Jian-hua,LI Hao-xia,YIN Yue,et al.Cloning and expression of acid invertase gene(LbAI) in wolfberry (Lycium barbarum L.)[J].,2015,(01):1140.[doi:doi:10.3969/j.issn.1000-4440.2015.05.031]
[3]沈婧丽,杨建国.滴灌条件下不同改良模式对碱化土壤性质和枸杞产量的影响[J].江苏农业学报,2020,(02):343.[doi:doi:10.3969/j.issn.1000-4440.2020.02.013]
　SHEN Jing-li,YANG Jian-guo.Effects of different improvement modes on the properties of alkalized soil and the yield of Chinese wolfberry under drip irrigation[J].,2020,(01):343.[doi:doi:10.3969/j.issn.1000-4440.2020.02.013]
[4]千春录,朱芹,高姗,等.外源褪黑素处理对采后水蜜桃冷藏品质及冷害发生的影响[J].江苏农业学报,2020,(03):702.[doi:doi:10.3969/j.issn.1000-4440.2020.03.024]
　QIAN Chun-lu,ZHU Qin,GAO Shan,et al.Effects of exogenous melatonin treatment on cold storage quality and chilling injury of postharvest peach fruit[J].,2020,(01):702.[doi:doi:10.3969/j.issn.1000-4440.2020.03.024]
[5]魏茜雅,林欣琪,梁腊梅,等.褪黑素引发处理提高朝天椒种子萌发及幼苗耐盐性的生理机制[J].江苏农业学报,2022,38(06):1637.[doi:doi:10.3969/j.issn.1000-4440.2022.06.023]
　WEI Xi-ya,LIN Xin-qi,LIANG La-mei,et al.Physiological mechanism of melatonin soaking on improving seed germination and seedling salt tolerance of pepper[J].,2022,38(01):1637.[doi:doi:10.3969/j.issn.1000-4440.2022.06.023]
[6]张雪莲,罗德旭,杨红,等.外源褪黑素和硒对高温胁迫下辣椒生理特性和抗氧化系统的影响[J].江苏农业学报,2023,(08):1729.[doi:doi:10.3969/j.issn.1000-4440.2023.08.013]
　ZHANG Xue-lian,LUO De-xu,YANG Hong,et al.Effects of exogenous melatonin and selenium on physiological properties and antioxidant systems of chilies under high temperature stress[J].,2023,(01):1729.[doi:doi:10.3969/j.issn.1000-4440.2023.08.013]
[7]宋燕芳,彭彤,马少兰,等.枸杞叶片酚酸类物质的自毒作用分析[J].江苏农业学报,2024,(02):213.[doi:doi:10.3969/j.issn.1000-4440.2024.02.003]
　SONG Yan-fang,PENG Tong,MA Shao-lan,et al.Analyzing the autotoxicity of phenolic acids from Lycium barbarum L. leaves[J].,2024,(01):213.[doi:doi:10.3969/j.issn.1000-4440.2024.02.003]
[8]任元龙,马蓉,王晓卓,等.叶面喷施褪黑素对盐胁迫下西瓜幼苗的影响[J].江苏农业学报,2024,(11):2140.[doi:doi:10.3969/j.issn.1000-4440.2024.11.018]
　REN Yuanlong,MA Rong,WANG Xiaozhuo,et al.Effects of foliar melatonin spray on watermelon seedlings under salt stress[J].,2024,(01):2140.[doi:doi:10.3969/j.issn.1000-4440.2024.11.018]

备注/Memo

备注/Memo:
收稿日期：2023-12-16基金项目：宁夏回族自治区自然科学基金项目(2022AAC03095)作者简介：杨建帮(1998-),男,宁夏同心人,硕士研究生,主要从事经济林栽培与生理等方面的研究。(E-mail)2225911860@qq.com通讯作者：冯美,(E-mail)feng_m@nxu.edu.cn
更新日期/Last Update: 2025-02-28