[1]刘念析,钱雪艳,邱红梅,等.大豆主要生育期基因型鉴定与分析[J].江苏农业学报,2024,(11):1992-2000.[doi:doi:10.3969/j.issn.1000-4440.2024.11.002]
　LIU Nianxi,QIAN Xueyan,QIU Hongmei,et al.Identification and analysis of major maturity genotypes in soybean[J].,2024,(11):1992-2000.[doi:doi:10.3969/j.issn.1000-4440.2024.11.002]
点击复制

大豆主要生育期基因型鉴定与分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
1992-2000
栏目:
遗传育种·生理生化
出版日期:
2024-11-30

文章信息/Info

Title:
Identification and analysis of major maturity genotypes in soybean
作者:
刘念析; 钱雪艳; 邱红梅; 刘月; 厉志; 刘佳; 王英男; 谭化; 刘晓冬; 王玉民; 董英山; 李玉秋
(吉林省农业科学院<中国农业科技东北创新中心>,吉林长春130033)
Author(s):
LIU Nianxi; QIAN Xueyan; QIU Hongmei; LIU Yue; LI Zhi; LIU Jia; WANG Yingnan; TAN Hua; LIU Xiaodong; WANG Yumin; DONG Yingshan; LI Yuqiu
(Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, China)
关键词:
大豆; 生育期; 基因型; 分子育种模型
Keywords:
soybean; growth period; genotype; molecular breeding model
分类号:
S565.101
DOI:
doi:10.3969/j.issn.1000-4440.2024.11.002
文献标志码:
A
摘要:
大豆是光周期敏感作物,大豆的生育期除了受到环境条件的影响,还受到遗传因素的调控。目前的研究发现,调控大豆开花和成熟的位点包括E1～E12和J基因,其中E1～E4是调控大豆开花和成熟的主要基因。本研究鉴定了250份大豆材料的E1～E4基因型及开花、成熟表型,通过关联分析发现,E1、E3基因与始花期、成熟期间的关系最密切,显性的E1、E3基因均有延迟开花的作用,隐性变异由于功能部分或完全丧失,导致开花提前。结果显示,大豆基因型组合可分为10组,分别为e1-nle2e4、e1-nlE2E3e4、e1-nle2E4、e1-nlE2E4、e1-asE2E4、e2e3e4、e1-ase2E4、E1e2E4、E1E2e3e4、E1E2E4,不同基因型组合决定了大豆生育期的多样化和对不同生态区的适应性。本研究结果为分子设计育种提供了分子模型,对于培育特定生态区的大豆品种具有重要意义。
Abstract:
Soybean is a photoperiod sensitive crop. The maturity of soybean is not only affected by environmental conditions, but also regulated by genetic factors. At present, E1-E12 and J genes were found to regulate the flowering and ripening of soybean, among which E1-E4 were the main genes regulating the flowering and ripening of soybean. In this study, 250 soybean materials with E1-E4 genotypes and flowering and maturity phenotypes were identified. Association analysis showed that E1 and E3 genes were most closely related to the first flowering and maturity stages. Dominant E1 and E3 genes delayed flowering, while recessive variation caused early flowering due to partial or complete loss of function. In this study, soybean genotype combinations could be divided into 10 groups, namely e1-nle2e4, e1-nlE2E3e4, e1-nle2E4, e1-nlE2E4, e1-asE2E4, e2e3e4, e1-ase2E4, E1e2E4, E1E2e3e4, E1E2E4. Different genotype combinations determined the diversification of soybean growth period and its adaptability to different ecological zones. The results of this study provide a molecular prediction model for molecular design breeding, which is important for breeding soybean varieties in specific ecological zones.

参考文献/References:

[1]杨如萍,宋雯雯,韦瑛,等. 甘肃省大豆品种生育期组的划分及地理分布研究[J]. 植物遗传资源学报,2021,22(2):349-360.
[2]LIN X Y, LIU B H, WELLER J L, et al. Molecular mechanisms for the photoperiodic regulation of flowering in soybean[J]. Journal of Integrative Plant Biology,2021,63(6):981-994.
[3]WU T T, LU S J, CAI Y P, et al. Molecular breeding for improvement of photothermal adaptability in soybean[J]. Molecular Breeding,2023,43(8):60.
[4]XIA Z J, WATANABE S, YAMADA T, et al. Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering[J]. Proceedings of the National Academy of Sciences of the United States of America,2012,109(32):E2155-E2164.
[5]WATANABE S, HIDESHIMA R, XIA Z J, et al. Map-based cloning of the gene associated with the soybean maturity locus E3[J]. Genetics,2009,182(4):1251-1262.
[6]WATANABE S, XIA Z J, HIDESHIMA R, et al. A map-based cloning strategy employing a residual heterozygous line reveals that the GIGANTEA gene is involved in soybean maturity and flowering[J]. Genetics,2011,188(2):395-407.
[7]LIU B H, KANAZAWA A, MATSUMURA H, et al. Genetic redundancy in soybean photoresponses associated with duplication of the phytochrome A gene[J]. Genetics,2008,180(2):995-1007.
[8]DISSANAYAKA A, RODRIGUEZ T O, DI S K, et al. Quantitative trait locus mapping of soybean maturity gene E5[J]. Breeding Science,2016,66(3):407-415.
[9]NISSAN N, COBER E R, SADOWSKI M, et al. Identifying new variation at the J locus,previously identified as e6,in long juvenile ‘Paranagoiana’ soybean[J]. Theoretical and Applied Genetics,2021,134(4):1007-1014.
[10]COBER E R, VOLDENG H D. A new soybean maturity and photoperiod-sensitivity locus linked to E1 and T[J]. Crop Science,2001,41(3):698-701.
[11]COBER E R, MOLNAR S J, CHARETTE M, et al. A new locus for early maturity in soybean[J]. Crop Science,2010,50(2):524-527.
[12]KONG F J, NAN H Y, CAO D, et al. A new dominant gene E9 conditions early flowering and maturity in soybean[J]. Crop Science,2014,54(6):2529-2535.
[13]SAMANFAR B, MOLNAR S J, CHARETTE M, et al. Mapping and identification of a potential candidate gene for a novel maturity locus,E10,in soybean[J]. Theoretical and Applied Genetics,2017,130(2):377-390.
[14]WANG F F, NAN H Y, CHEN L Y, et al. A new dominant locus,E11,controls early flowering time and maturity in soybean[J]. Molecular Breeding,2019,39(5):70.
[15]LU S J, ZHAO X H, HU Y L, et al. Natural variation at the soybean J locus improves adaptation to the tropics and enhances yield[J]. Nature Genetics,2017,49:773-779.
[16]TANG Y, LU S J, FANG C, et al. Diverse flowering responses subjecting to ambient high temperature in soybean under short-day conditions[J]. Plant Biotechnology Journal,2023,21(4):782-791.
[17]WANG L S, LI H Y, HE M L, et al. GIGANTEA orthologs,E2 members,redundantly determine photoperiodic flowering and yield in soybean[J]. Journal of Integrative Plant Biology,2023,65(1):188-202.
[18]TSUBOKURA Y, WATANABE S, XIA Z J, et al. Natural variation in the genes responsible for maturity loci E1,E2,E3 and E4 in soybean[J]. Annals of Botany,2014,113(3):429-441.
[19]TSUBOKURA Y, MATSUMURA H, XU M L, et al. Genetic variation in soybean at the maturity locus E4 is involved in adaptation to long days at high latitudes[J]. Agronomy,2013,3(1):117-134.
[20]TARDIVEL A, SONAH H, BELZILE F, et al. Rapid identification of alleles at the soybean maturity gene E3 using genotyping by sequencing and a haplotype-based approach[J]. The Plant Genome,2014,7(2):1-9.
[21]ZHAI H, LYU S X, WANG Y Q, et al. Allelic variations at four major maturity E genes and transcriptional abundance of the E1 gene are associated with flowering time and maturity of soybean cultivars[J]. PLoS One,2014,9(5):e97636.
[22]LI J C, WANG X B, SONG W W, et al. Genetic variation of maturity groups and four E genes in the Chinese soybean mini core collection[J]. PLoS One,2017,12(2):e0172106.
[23]JIANG B J, NAN H Y, GAO Y F, et al. Allelic combinations of soybean maturity Loci E1,E2,E3 and E4 result in diversity of maturity and adaptation to different latitudes[J]. PLoS One,2014,9(8):e106042.
[24]KURASCH A K, HAHN V, LEISER W L, et al. Identification of mega-environments in Europe and effect of allelic variation at maturity E loci on adaptation of European soybean[J]. Plant,Cell & Environment,2017,40(5):765-778.
[25]ABUGALIEVA S, DIDORENKO S, ANUARBEK S, et al. Assessment of soybean flowering and seed maturation time in different latitude regions of Kazakhstan[J]. PLoS One,2016,11(12):e0166894.
[26]LANGEWISCH T, LENIS J, JIANG G L, et al. The development and use of a molecular model for soybean maturity groups[J]. BMC Plant Biology,2017,17(1):91.
[27]YERZHEBAYEVA R, DIDORENKO S, AMANGELDIYEVA A, et al. Marker-assisted selection for early maturing E loci in soybean yielded prospective breeding lines for high latitudes of northern Kazakhstan[J]. Biomolecules,2023,13(7):1146.
[28]刘月,齐广勋,桑永生,等. 山东省3个野生大豆居群生育期基因遗传多样性分析[J]. 植物遗传资源学报,2020,21(3):560-567.

相似文献/References:

[1]刘朝茂,李成云.玉米与大豆间作对玉米叶片衰老的影响[J].江苏农业学报,2017,(02):322.[doi:doi:10.3969/j.issn.1000-4440.2017.02.013]
　LIU Chao-mao,LI Cheng-yun.Effects of maize/soybean intercropping on maize leaf senescence[J].,2017,(11):322.[doi:doi:10.3969/j.issn.1000-4440.2017.02.013]
[2]张令瑄,谢婷婷,王瑾,等.大田条件下ＵＶ-Ｂ 辐射增强对大豆根际土壤相关指标的影响[J].江苏农业学报,2016,(01):118.[doi:10.3969/j.issn.1000-4440.2016.01.018]
　ZHANG Ling-xuan,XIE Ting-ting,WANG Jin,et al.Soybean rhizosphere soil parameters in response to enhanced UV-B radiation under field condition[J].,2016,(11):118.[doi:10.3969/j.issn.1000-4440.2016.01.018]
[3]宁丽华,何晓兰,张大勇.大豆耐盐相关基因GmNcl1功能标记的开发及验证[J].江苏农业学报,2017,(06):1227.[doi:doi:10.3969/j.issn.1000-4440.2017.06.005]
　NING Li-hua,HE Xiao-lan,ZHANG Da-yong.Development and validation of the function marker of soybean salt tolerance gene GmNcl1[J].,2017,(11):1227.[doi:doi:10.3969/j.issn.1000-4440.2017.06.005]
[4]杨艳丽,杨勇,李大红,等.转桃PpCuZnSOD基因大豆的耐旱性[J].江苏农业学报,2018,(05):978.[doi:doi:10.3969/j.issn.1000-4440.2018.05.003]
　YANG Yan-li,YANG Yong,LI Da-hong,et al.Drought tolerance of transgenic soybean with PpCuZnSOD gene[J].,2018,(11):978.[doi:doi:10.3969/j.issn.1000-4440.2018.05.003]
[5]闫会,张允刚,刘亚菊,等.生育期对徐紫薯8号品质及结薯性的影响[J].江苏农业学报,2019,(01):9.[doi:doi:10.3969/j.issn.1000-4440.2019.01.002]
　YAN Hui,ZHANG Yun-gang,LIU Ya-ju,et al.Effects of growth stage on quality and tuber traits of new sweet potato cultivar Xuzishu8[J].,2019,(11):9.[doi:doi:10.3969/j.issn.1000-4440.2019.01.002]
[6]孙彦坤,陈睿,李静,等.不同降雨年型下反枝苋和大豆光合特征的比较[J].江苏农业学报,2019,(03):554.[doi:doi:10.3969/j.issn.1000-4440.2019.03.008]
　SUN Yan-kun,CHEN Rui,LI Jing,et al.Comparison of photosynthetic characteristics between Amaranthus retroexus and Glycine max under different annual rainfall pattern[J].,2019,(11):554.[doi:doi:10.3969/j.issn.1000-4440.2019.03.008]
[7]曹媛媛,陈春,郭婷婷,等.亲和性促生菌DW12-L的定殖及其对大豆生长的影响[J].江苏农业学报,2019,(04):776.[doi:doi:10.3969/j.issn.1000-4440.2019.04.004]
　CAO Yuan yuan,CHEN Chun,GUO Ting ting,et al.Colonization of soybean affinity rhizobacteria strain DW12-L and its effects on soybean growth[J].,2019,(11):776.[doi:doi:10.3969/j.issn.1000-4440.2019.04.004]
[8]丁俊男,于少鹏,李鑫,等.生物炭对大豆生理指标和农艺性状的影响[J].江苏农业学报,2019,(04):784.[doi:doi:10.3969/j.issn.1000-4440.2019.04.005]
　DING Jun nan,YU Shao peng,LI Xin,et al.Effects of biochar application on soybean physiological indices and agronomic traits[J].,2019,(11):784.[doi:doi:10.3969/j.issn.1000-4440.2019.04.005]
[9]单捷,孙玲,王志明,等.GF-1影像遥感监测指标与冬小麦长势参数的关系[J].江苏农业学报,2019,(06):1323.[doi:doi:10.3969/j.issn.1000-4440.2019.06.008]
　SHAN Jie,SUN Ling,WANG Zhi-ming,et al.Relationship between remote sensing monitoring indices and growth parameters in winter wheat based on GF-1 images[J].,2019,(11):1323.[doi:doi:10.3969/j.issn.1000-4440.2019.06.008]
[10]曹帅,杜仲阳,刘鹏,等.碱胁迫对大豆光合特性及内源激素含量的影响[J].江苏农业学报,2020,(02):284.[doi:doi:10.3969/j.issn.1000-4440.2020.02.005]
　CAO Shuai,DU Zhong-yang,LIU Peng,et al.Effects of alkaline stress on photosynthetic characteristics and endogenous hormone contents of soybean[J].,2020,(11):284.[doi:doi:10.3969/j.issn.1000-4440.2020.02.005]

备注/Memo

备注/Memo:
收稿日期:2024-01-30 基金项目:国家重点研发计划政府间国际合作项目(2022YFE0130300) 作者简介:刘念析(1987-),女,山东高密人,博士研究生,副研究员,主要从事大豆遗传育种及抗病鉴定研究。(E-mail)lnx69@126.com。钱雪燕为共同第一作者。通讯作者:李玉秋,(E-mail)lyqhb1@126.com；董英山,(E-mail)ysdong@cjaas.com
更新日期/Last Update: 2025-01-20