[1]段昱,胥红,吴雪儿,等.马蓝水培扦插生根技术优化及其生理生化响应机制[J].江苏农业学报,2026,42(08):1664-1670.[doi:doi:10.3969/j.issn.1000-4440.2026.08.015]
　DUAN Yu,XU Hong,WU Xueer,et al.Optimization of hydroponic cutting rooting technology for Baphicacanthus cusia (Nees) Bremek. and its physiological and biochemical response mechanisms[J].,2026,42(08):1664-1670.[doi:doi:10.3969/j.issn.1000-4440.2026.08.015]
点击复制

马蓝水培扦插生根技术优化及其生理生化响应机制()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年08期
页码:
1664-1670
栏目:
园艺
出版日期:
2026-08-31

文章信息/Info

Title:
Optimization of hydroponic cutting rooting technology for Baphicacanthus cusia (Nees) Bremek. and its physiological and biochemical response mechanisms
作者:
段昱1; 胥红1; 吴雪儿1; 秦英1; 张祎晨1; 高志波2; 陈洋尔1
(1.四川农业大学生命科学学院,四川雅安625014；2.芦山县益明药业有限公司,四川雅安625600)
Author(s):
DUAN Yu1; XU Hong1; WU Xue’er1; QIN Ying1; ZHANG Yichen1; GAO Zhibo2; CHEN Yanger1
(1.College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China；2.Yiming Pharmaceutical Co., Ltd. of Lushan County, Ya’an 625600, China)
关键词:
马蓝; 扦插; 水培; 外源激素; 生根效果; 抗氧化酶
Keywords:
Baphicacanthus cusia (Nees) Bremek.; cutting; hydroponic culture; exogenous hormone; rooting effect; antioxidant enzyme
分类号:
S339.4
DOI:
doi:10.3969/j.issn.1000-4440.2026.08.015
文献标志码:
A
摘要:
本研究以云南引种的马蓝栽培品种为材料,系统分析插条部位、长度以及外源激素种类、质量浓度和处理时间对水培马蓝插条生根效果、营养物质含量、抗氧化酶活性及内源激素水平的影响。结果表明,取自马蓝枝条顶端、长度为15 cm的插条生根能力最强,200 mg/L吲哚丁酸处理2 h的马蓝插条生根效果最佳。生理响应方面,在马蓝繁殖初期,插条内可溶性糖、可溶性淀粉含量均大幅下降,其中可溶性淀粉含量降幅最大,表明淀粉作为主要能量来源被大量降解为可溶性糖。吲哚丁酸处理后,马蓝插条超氧化物歧化酶活性较低,表明插条内活性氧水平较低,内部保持氧化还原稳态平衡。在不定根伸长期（扦插后21～28 d）,吲哚丁酸处理的马蓝插条赤霉素含量显著低于CK(P＜0.05),有利于不定根的快速伸长。上述营养代谢、酶活性及激素水平的协同变化有效促进了马蓝插条不定根的形成。本研究为建立马蓝高效水培扦插繁殖技术体系提供了理论依据。
Abstract:
This study systematically investigated the effects of cutting position, cutting length, and exogenous hormone type, mass concentration, as well as treatment duration on rooting performance, nutrient content, antioxidant enzyme activities, and endogenous hormone levels in hydroponically cultivated Baphicacanthus cusia (Nees) Bremek. cuttings, using a cultivar introduced from Yunnan province as the experimental material. The results showed that cuttings taken from the apical part of the shoot with a length of 15 cm exhibited the strongest rooting ability, and the optimal rooting effect was achieved by treating the cuttings with 200 mg/L indole-3-butyric acid (IBA) for two hours. Regarding the physiological responses, the contents of soluble sugar and soluble starch in the cuttings decreased significantly during the early stage of propagation, among which soluble starch content showed the greatest decrease, indicating that starch served as the primary energy source and was substantially degraded into soluble sugars. Following IBA treatment, the cuttings of Baphicacanthus cusia (Nees) Bremek. exhibited low superoxide dismutase activity, suggesting a low level of reactive oxygen species and the maintenance of a redox homeostasis within the cuttings. During the adventitious root elongation stage (21-28 days after cutting), the gibberellin content in IBA-treated cuttings was significantly lower than that in the control(P＜0.05), which facilitated rapid adventitious root elongation. The synergistic changes in nutrient metabolism, enzyme activities, and hormone levels effectively promoted adventitious root formation in Baphicacanthus cusia (Nees) Bremek. cuttings. This study provides a theoretical basis for establishing an efficient hydroponic cutting propagation system for Baphicacanthus cusia (Nees) Bremek.

参考文献/References:

[1]南京中医药大学. 中药大辞典[M]. 上海:上海科学技术出版社,2006.
[2]代邦元. 板蓝根种植把“五关”[J]. 农家科技,2002(5):32.
[3]国家药典委员会. 中华人民共和国药典2020年版一部[M]. 北京:中国医药科技出版社,2020.
[4]杜沛欣. 马蓝(南板蓝根)的生物学特性研究[D]. 广州:广州中医药大学,2008.
[5]孙翠萍. 南板蓝根品质评价研究[D]. 成都:成都中医药大学,2012.
[6]蔡鹏,谢强,樊湘宁,等. 木槿插条的悬空水培研究[J]. 黑龙江农业科学,2015(2):66-67.
[7]刘燕花,陈福生,邓莹,等. 薄荷扦插水培研究[J]. 安徽农业科学,2025,53(11):43-46,50.
[8]Schuler J L, McCarthy W. Development of eastern cottonwood cuttings as modified by cutting length and surface area available for rooting[J]. New Forests,2015,46:547-559.
[9]袁利利. 华北五角枫扦插繁殖技术及生根机理的研究[D]. 泰安:山东农业大学,2012.
[10]刘式超,周再知,张金浩,等. 裸花紫珠嫩枝扦插生根影响因子研究[J]. 植物研究,2016,36(5):739-746.
[11]令狐昱慰,黎斌,张莹,等. 不同处理对鸡麻扦插繁殖的影响[J]. 西北农业学报,2013,22(9):163-166.
[12]Dubois M, Gilles K, Hamilton J K, et al. A colorimetric method for the determination of sugars[J]. Nature,1951,168:167.
[13]Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976,72(1/2):248-254.
[14]Beyer W J, Fridovich I. Assaying for superoxide dismutase activity:some large consequences of minor changes in conditions[J]. Analytical Biochemistry,1987,161(2):559-566.
[15]蒋虹,张健,王景明,等. 《植物生理学实验指导》(第5版)中的几个不足之处[J]. 植物生理学报,2020,56(1):11-15.
[16]Zhu Y C, Liao W B. The metabolic constituent and rooting-related enzymes responses of marigold explants to hydrogen gas during adventitious root development[J]. Theoretical and Experimental Plant Physiology,2017,29:77-85.
[17]史树德,孙亚卿,魏磊,等. 植物生理学实验指导[J]. 北京:中国林业出版社,2012.
[18]Zeng H, Yang W H, Lu C Z, et al. Effect of CPPU on carbohydrate and endogenous hormone levels in young macadamia fruit[J]. PLoS One,2016,11(7):e0158705.
[19]廖林正,周友兵,吴涤,等. 生长调节物质及木质化程度对金银花扦插繁殖的影响[J]. 北方园艺,2012(4):161-163.
[20]姚耸峰. ‘高山朝霞’月季嫩枝扦插繁殖技术及生根机理研究[D]. 长春:吉林农业大学,2022.
[21]王青. 麻楝嫩枝扦插繁殖技术及其生根机理研究[D]. 哈尔滨:东北林业大学,2019.
[22]曾庆钱,杨红梅,黄珊珊,等. 南板蓝扦插繁殖技术研究[J]. 亚热带植物科学,2011,40(4):71-74.
[23]闫文涛. 蒙古栎嫩枝扦插繁殖技术及其生根机理的研究[D]. 沈阳:沈阳农业大学,2017.
[24]张金浩. 裸花紫珠扦插繁殖技术及生根机理研究[D]. 北京:中国林业科学研究院,2014.

相似文献/References:

[1]李敏,王朴,路喆,等.不同激素、处理时间及基质对两种大马士革玫瑰扦插生根率的影响[J].江苏农业学报,2016,(01):207.[doi:10.3969/j.issn.1000-4440.2016.01.032 ]
　LI Min,WANG Pu,LU Zhe,et al.Effects of hormones, treating time and substrates on cutting rooting rates in two varieties of Rosa damascena Mill.[J].,2016,(08):207.[doi:10.3969/j.issn.1000-4440.2016.01.032 ]

备注/Memo

备注/Memo:
收稿日期：2025-12-30基金项目：国家自然科学基金面上项目(32572246)；四川省中医药管理局科学技术研究专项课题(2023MS344)；芦山县校地合作项目作者简介：段昱(2001-),男,四川自贡人,硕士研究生,研究方向为中药材马蓝繁育和抗逆机制。(E-mail)1325551978@qq.com通讯作者：陈洋尔,(E-mail)anty9826@163.com
更新日期/Last Update: 2026-09-16