[1]卢倩倩,张蓉,高永生,等.高丹草SsNR基因的克隆、分子特征与表达分析[J].江苏农业学报,2026,42(08):1654-1663.[doi:doi:10.3969/j.issn.1000-4440.2026.08.014]
　LU Qianqian,ZHANG Rong,GAO Yongsheng,et al.Cloning, molecular characterization, and expression pattern analysis of the SsNR gene in Sorghum bicolor×sudanense[J].,2026,42(08):1654-1663.[doi:doi:10.3969/j.issn.1000-4440.2026.08.014]
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高丹草SsNR基因的克隆、分子特征与表达分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年08期
页码:
1654-1663
栏目:
园艺
出版日期:
2026-08-31

文章信息/Info

Title:
Cloning, molecular characterization, and expression pattern analysis of the SsNR gene in Sorghum bicolor×sudanense
作者:
卢倩倩1; 2; 张蓉1; 高永生1; 2; 杨东升1; 2; 李雨芯1; 白皓月1; 张舒1
(1.河套学院农牧与食品学院,内蒙古巴彦淖尔015000；2.河套地区绿色农产品安全生产与预警控制实验室,内蒙古巴彦淖尔015000)
Author(s):
LU Qianqian1; 2; ZHANG Rong1; GAO Yongsheng1; 2; YANG Dongsheng1; 2; LI Yuxin1; BAI Haoyue1; ZHANG Shu1
(1.College of Agriculture, Animal Husbandry and Food, Hetao College, Bayannur 015000, China;2.Laboratory of Safety Production and Early Warning Control for Green Agricultural Products in Hetao Region, Bayannur 015000, China)
关键词:
高丹草; SsNR基因; 生物信息学; 基因克隆
Keywords:
Sorghum bicolor×sudanense; SsNR gene; bioinformatics; gene cloning
分类号:
S544+.9
DOI:
doi:10.3969/j.issn.1000-4440.2026.08.014
文献标志码:
A
摘要:
硝酸还原酶(NR)作为植物从土壤中摄取NO-3的关键转运蛋白,在植物的生长发育及生殖阶段发挥着至关重要的作用。为深入探究高丹草氮元素转运机制,本研究依托不同蛋白质含量高丹草的转录组数据,筛选得到NR基因,将其命名为SsRN,并对该基因的分子特征、结构以及系统进化树展开分析,剖析了该基因在不同高丹草材料叶片中的表达情况,并对其不同生育期、不同器官进行荧光定量分析。研究结果显示,高丹草NR基因序列全长2 679 bp,编码892个氨基酸,该基因不含信号肽且不存在跨膜区,SsNR蛋白主要定位在过氧化物酶体和叶绿体中。系统进化分析结果表明,高丹草SsNR基因与玉米的亲缘关系最近。SsNR基因在高丹草拔节期各器官中的相对表达量显著高于三叶期,拔节期在叶片中的相对表达量最高,三叶期在根中的相对表达量最高。本研究结果为揭示高丹草SsNR基因的功能及相关调控机制提供了理论依据,对高丹草氮素利用效率的遗传改良具有重要指导意义。
Abstract:
Nitrate reductase (NR) serves as a key transport protein for plants to absorb NO-3 from the soil and plays a crucial role in the growth, development, and reproductive stages of plants. To deeply explore the nitrogen element transport mechanism of Sorghum bicolor×sudanense, this study relied on the transcriptomic data of different protein content Sorghum bicolor×sudanense from previous research to screen out the NR gene, which was named SsNR. The molecular characteristics, structure, and phylogenetic tree of this gene were analyzed, and the expression profile of the gene in the leaves of different Sorghum bicolor×sudanense varieties was dissected. Fluorescence quantitative analysis was also conducted on different tissues at different growth stages. The research results showed that the full-length sequence of the NR gene in Sorghum bicolor×sudanense was 2 679 bp, encoding 892 amino acids. The gene did not contain a signal peptide and had no transmembrane region. The preliminary subcellular localization was mainly in the peroxisome and chloroplast. The results of the phylogenetic analysis indicated that the SsNR gene of Sorghum bicolor×sudanense was the most closely related to that of corn. The expression level of the gene in various organs of Sorghum bicolor×sudanense at the jointing stage was significantly higher than that at the three-leaf stage, with the highest relative expression in the leaves at the jointing stage and in the roots at the three-leaf stage. This study provides a theoretical basis for revealing the function and related regulatory mechanism of the SsNR gene in Sorghum bicolor×sudanense and has significant guiding significance for the genetic improvement of nitrogen use efficiency in Sorghum bicolor×sudanense.

参考文献/References:

[1]王慧婷,徐文锋,田振平,等. 高丹草营养品质性状的QTL定位及候选基因筛选[J]. 植物遗传资源学报,2025,26(5):1031-1041.
[2]Lu Q Q, Yu X X, Wang H T, et al. Quantitative trait locus mapping for important yield traits of a sorghum-sudangrass hybrid using a high-density single nucleotide polymorphism map[J]. Frontiers in Plant Science,2022,13:1098605.
[3]洪森荣,刘佳凝,袁昕,等. 苏丹草和高丹草转录组测序及其差异基因表达分析[J]. 草地学报,2024,32(3):714-725.
[4]董婧,逯晓萍,米福贵,等. 高丹草杂种和亲本叶片基因差异表达研究[J]. 植物遗传资源学报,2016,17(4):738-747.
[5]李源,赵海明,游永亮,等. 高丹草“土-草-畜”界面的氮素运移规律[J]. 中国生态农业学报(中英文),2022,30(8):1249-1257.
[6]钟楚,简少芬. 氮素营养调控植物逆境适应的机理研究[J]. 华北农学报,2020,35(增刊1):424-432.
[7]白文钦,胡明瑜,王春萍,等. 氮素在植物中的利用综述[J]. 江苏农业科学,2020,48(4):1-11.
[8]于铁峰,刘晓静,郝凤,等. NO-3-N/NH+4-N配比对紫花苜蓿营养品质及饲用价值的影响研究[J]. 草业学报,2016,25(12):102-110.
[9]王照兰,杜建材,李青丰. 12份苜蓿种质材料的氨基酸分析[J]. 中国草地,2004,48(1):28-33.
[10]卢倩倩. 高丹草超高密度遗传图谱构建及营养品质性状QTL定位与蛋白质相关候选基因挖掘[D]. 呼和浩特:内蒙古农业大学,2023.
[11]Solomon C M, Collier J L, Berg G M, et al. Role of urea in microbial metabolism in aquatic systems:a biochemical and molecular review[J]. Aquatic Microbial Ecology,2010,59:67-88.
[12]马晓霞,秘一先,陈宏亮,等. 14个引进紫花苜蓿品种在宁夏引黄灌区的生产性能和营养价值综合分析[J]. 草业科学,2022,39(2):328-342.
[13]何金环,李凤玲,王延方. 牧草叶绿素含量与蛋白质含量的相关性研究[J]. 河南农业科学,2015,44(2):139-141.
[14]方嘉琪,赵妍,周青平,等. 低氮胁迫下作物谷氨酰胺合成酶—谷氨酸合成酶循环的研究进展[J]. 草业科学,2025,42(4):1036-1050.
[15]段永康,杨海燕,吴文龙,等. 植物氮素吸收、转运和同化的分子机制[J]. 福建农业学报,2022,37(4):547-554.
[16]Konishi M, Yanagisawa S. Roles of the transcriptional regulation mediated by the nitrate-responsive cis-element in higher plants[J]. Biochemical and Biophysical Research Communications,2011,411(4):708-713.
[17]Kaur B, Asthir B, Bains N S. Enzymatic efficiency and genotypic differences for nitrogen assimilation in wheat[J]. Proceedings of the National Academy of Sciences,India Section B:Biological Sciences,2017,87(3):985-995.
[18]Sharma P K, Gurjar R, Kumar M, et al. Combined effect of nitrogen and phosphorus fertilizer on the activity of the nitrate reductase enzyme in different wheat cultivars[J]. International Journal of Environment and Climate Change,2023，13（1）:196-204.
[19]Gao Z Y, Wang Y F, Chen G, et al. The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency[J]. Nature Communications,2019,10:5207.
[20]Zhang Z H, Chu C C. Nitrogen-use divergence between indica and Japonica rice:variation at nitrate assimilation[J]. Molecular Plant,2020,13(1):6-7.
[21]Han M L, Lü Q Y, Zhang J, et al. Decreasing nitrogen assimilation under drought stress by suppressing DST-mediated activation of Nitrate Reductase 1.2 in rice[J]. Molecular Plant,2022,15(1):167-178.
[22]马桂香,门中华. 氮素对大豆硝酸还原酶及根系活力的影响[J]. 山东农业大学学报(自然科学版),2014,45(5):656-659.
[23]张瑛,李浩,张雨萱,等. 水稻氯酸钾抗性与硝酸还原酶NR、亚硝酸还原酶NIR的关联性研究[J]. 中国农学通报,2019,35(14):1-7.
[24]卢倩倩,于卓,王慧婷,等. 基于RNA-seq技术挖掘与高丹草粗蛋白合成相关的基因[J]. 基因组学与应用生物学,2022,41(11):2182-2194.
[25]牛亚青青. 13个高丹草系列品种产量品质性状及细胞学和SSR分析[D]. 呼和浩特:内蒙古农业大学,2020.
[26]赵永,王帅奇,何禹鑫,等. 蓖麻RcCPK26基因克隆、生物信息学及组织表达分析[J/OL]. 分子植物育种,2025:1-13. https://link.cnki.net/urlid/46.1068.S.20241223.1434.006.
[27]栾鑫超,岳文俊,李连杰,等. 大豆GmMYB73基因的克隆及生物信息学分析[J/OL]. 吉林农业大学学报,2025:1-7. http://kns.cnki.net/kcms/detail/22.1100.s.
[28]刘春梅,董曦文,严跃辉,等. 叶面喷硒对不同氮水平下寒地水稻氮代谢和产量的影响[J]. 农业环境科学学报,2025,44(9):2253-2263.
[29]孙淑斌,罗金葵,徐国华,等. 小白菜硝酸还原酶基因的克隆与初步鉴定[J]. 植物营养与肥料学报,2006,12(4):592-596.
[30]晏娟,沈其荣,尹斌,等. 应用15N示踪技术研究水稻对氮肥的吸收和分配[J]. 核农学报,2009,23(3):487-491,496.
[31]徐明杰,张琳,汪新颖,等. 不同管理方式对夏玉米氮素吸收、分配及去向的影响[J]. 植物营养与肥料学报,2015,21(1):36-45.
[32]马兴华,于振文,梁晓芳,等. 施氮量和底追比例对小麦氮素利用和土壤硝态氮含量的影响[J]. 水土保持学报,2006,20(5):95-98.

备注/Memo

备注/Memo:
收稿日期：2025-10-24基金项目：内蒙古青年自然科学基金项目(2024QN03056)；巴彦淖尔区域创新重点联合基金项目(2025QYCX01)；河套学院高层次人才引进科研启动经费资助项目(HYRC202311)作者简介：卢倩倩(1994-),女,内蒙古兴和人,博士,讲师,主要从事饲用作物遗传育种研究。(E-mail)lqq15847755064@163.com通讯作者：高永生,(E-mail)yongshenggao@aliyun.com
更新日期/Last Update: 2026-09-16