[1]唐璇,吕雪莹,揭红东,等.辣椒CaCHI与CaCHS2基因的分子克隆及生物信息学、异源表达功能分析[J].江苏农业学报,2026,42(08):1637-1644.[doi:doi:10.3969/j.issn.1000-4440.2026.08.012]
 TANG Xuan,LYU Xueying,JIE Hongdong,et al.Molecular cloning, bioinformatics and heterologous expression functional analysis of CaCHI and CaCHS2 genes from Capsicum annuum L.[J].,2026,42(08):1637-1644.[doi:doi:10.3969/j.issn.1000-4440.2026.08.012]
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辣椒CaCHI与CaCHS2基因的分子克隆及生物信息学、异源表达功能分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年08期
页码:
1637-1644
栏目:
园艺
出版日期:
2026-08-31

文章信息/Info

Title:
Molecular cloning, bioinformatics and heterologous expression functional analysis of CaCHI and CaCHS2 genes from Capsicum annuum L.
作者:
唐璇1; 2; 吕雪莹1; 3; 揭红东1; 4; 谢志玉1; 2; 何鹏亮1; 2; 尹伟丹5; 揭雨成1; 2
(1.湖南农业大学苎麻研究所,湖南长沙410128;2.湖南省草类作物种质创新与利用工程技术研究中心,湖南长沙410128;3.曲阜远东职业技术学院,山东济宁273100;4.湖南省作物研究所,湖南长沙410128;5.怀化市农业农村局,湖南怀化418000)
Author(s):
TANG Xuan1; 2; LYU Xueying1; 3; JIE Hongdong1; 4; XIE Zhiyu1; 2; HE Pengliang1; 2; YIN Weidan5; JIE Yucheng1; 2
(1.Institute of Ramie, Hunan Agricultural University, Changsha 410128, China;2.Hunan Engineering Technology Research Center for Germplasm Innovation and Utilization of Grass Crops, Changsha 410128, China;3.Qufu Fareast Vocational and Technical College, Jining 273100, China;4.Crop Research Institute of Hunan Province, Changsha 410128, China;5.Huaihua Bureau of Agriculture and Rural Affairs, Huaihua 418000, China)
关键词:
辣椒; 查尔酮异构酶基因(CHI); 查尔酮合酶基因(CHS); 异源表达; 类黄酮; 干旱胁迫
Keywords:
Capsicum annuum L.; chalcone isomerase gene (CHI); chalcone synthase gene (CHS); heterologous expression; flavonoids; drought stress
分类号:
S641.3
DOI:
doi:10.3969/j.issn.1000-4440.2026.08.012
文献标志码:
A
摘要:
查尔酮合酶(CHS)和查尔酮异构酶(CHI)是类黄酮生物合成途径中的关键酶。本研究克隆了辣椒CaCHS2与CaCHI基因,进行生物信息学分析,并构建过表达载体转化拟南芥。结果显示,分别过表达CaCHS2与CaCHI基因的拟南芥株系叶片面积增大、颜色加深,种皮颜色深于野生型。干旱条件下,分别过表达CaCHS2与CaCHI基因的拟南芥株系的类黄酮含量和鲜质量均显著高于野生型(P<0.05)。而CHI基因缺失拟南芥株系在干旱胁迫下死亡。表明CaCHS2与CaCHI可通过促进类黄酮积累增强植株抗旱性。本研究结果为辣椒类黄酮代谢调控机制解析及抗逆分子育种提供了理论参考。
Abstract:
Chalcone synthase (CHS) and chalcone isomerase (CHI) are key enzymes in the flavonoid biosynthetic pathway. In this study, the pepper genes CaCHS2 and CaCHI were cloned and subjected to bioinformatic analysis, and overexpression vectors were constructed for transformation into Arabidopsis thaliana. The results showed that the Arabidopsis lines overexpressing CaCHS2 and CaCHI exhibited larger and darker green leaves, as well as darker seed coats compared with the wild type. Under drought conditions, the flavonoid content and fresh weight of transgenic lines were significantly higher than those of the wild type (P<0.05). In contrast, the Arabidopsis lines lacking the CHI gene could not survive under drought stress. These findings indicate that CaCHS2 and CaCHI improve plant drought resistance by promoting flavonoid accumulation. This study provides a theoretical basis for elucidating the regulatory mechanism of flavonoid metabolism and molecular breeding for stress tolerance in pepper.

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备注/Memo

备注/Memo:
收稿日期:2026-03-13基金项目:国家重点研发计划项目(2019YFD1002205-3)作者简介:唐璇(2003-),男,湖南怀化人,硕士研究生,主要从事辣椒生长和次生代谢产物相关研究工作。(E-mail)3162186963@qq.com通讯作者:揭雨成,(E-mail)ibfcjyc@vip.sina.com
更新日期/Last Update: 2026-09-16