[1]胡嘉琦,张兰,张凡刘莎莎,等.牦牛肠道血管细胞黏附分子-1(VCAM-1)、低氧诱导因子-1α(HIF-1α)及白细胞介素-4(IL-4)的增龄性差异[J].江苏农业学报,2026,42(08):1624-1636.[doi:doi:10.3969/j.issn.1000-4440.2026.08.011]
　HU Jiaqi,ZHANG Lan,ZHANG Fan,et al.Age-related differences in vascular cell adhesion molecule-1 （VCAM-1）, hypoxia-inducible factor-1α (HIF-1α) and interleukin-4 (IL-4) in the intestine of yaks[J].,2026,42(08):1624-1636.[doi:doi:10.3969/j.issn.1000-4440.2026.08.011]
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牦牛肠道血管细胞黏附分子-1(VCAM-1)、低氧诱导因子-1α(HIF-1α)及白细胞介素-4(IL-4)的增龄性差异()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年08期
页码:
1624-1636
栏目:
畜牧兽医·水产养殖
出版日期:
2026-08-31

文章信息/Info

Title:
Age-related differences in vascular cell adhesion molecule-1 （VCAM-1）, hypoxia-inducible factor-1α (HIF-1α) and interleukin-4 (IL-4) in the intestine of yaks
作者:
胡嘉琦1; 张兰2; 张凡1刘莎莎1; 王军1; 谈笑1; 蓝雯琳1; 陈伟基1; 杨琨1
(1.西北民族大学生命科学与工程学院,甘肃兰州730030；2.甘肃民族师范学院化学与生命科学系,甘肃合作747000)
Author(s):
HU Jiaqi1; ZHANG Lan2; ZHANG Fan1; LIU Shasha1; WANG Jun1; TAN Xiao1; LAN Wenlin1; CHEN Weiji1; YANG Kun1
(1.School of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China；2.Department of Chemistry and Life Science, Gansu Normal University for Nationalities, Hezuo 747000, China)
关键词:
牦牛; 小肠; 增龄性; 低氧; 血管细胞黏附分子-1(VCAM-1); 低氧诱导因子-1α(HIF-1α); 白细胞介素-4
Keywords:
yak; small intestine; aging; hypoxia; vascular cell adhesion molecule-1 (VCAM-1); hypoxia-inducible factor-1α (HIF-1α); interleukin-4 (IL-4)
分类号:
S823.85
DOI:
doi:10.3969/j.issn.1000-4440.2026.08.011
文献标志码:
A
摘要:
为明确牦牛肠道血管细胞黏附分子-1(VCAM-1)、低氧诱导因子-1α(HIF-1α)和白细胞介素-4(IL-4)的增龄性差异,本研究选取新生、五月龄及成年3个年龄组的健康牦牛小肠组织(回肠、空肠和十二指肠),采用苏木精-伊红(H&E)染色法、马松(Masson)三色染色法、过碘酸-雪夫(PAS)染色法以及免疫组织化学分析方法探究血管细胞黏附分子-1、低氧诱导因子-1α和白细胞介素-4在牦牛肠道的表达与分布特征及增龄性差异。结果表明,新生期牦牛十二指肠绒毛高度与隐窝深度比值(V/C)显著高于空肠和回肠；胶原纤维主要分布于十二指肠腺区域及固有层,新生期牦牛十二指肠胶原纤维占比显著高于五月龄和成年期牦牛,且新生期牦牛十二指肠胶原纤维占比显著高于回肠和空肠；潘氏细胞PAS染色平均光密度(AOD)较高,呈强阳性，黏液细胞、杯状细胞及纹状缘PAS染色呈弱阳性。VCAM-1主要分布在牦牛小肠绒毛黏膜层的吸收细胞、淋巴细胞与纹状缘中,牦牛小肠多部位HIF-1α呈阳性表达,IL-4富集于牦牛小肠基膜、肌纤维以及淋巴细胞中。随着年龄增大,牦牛回肠VCAM-1阳性区域AOD、HIF-1α阳性区域AOD和IL-4阳性区域AOD均呈显著减少趋势,空肠和十二指肠VCAM-1阳性区域AOD和HIF-1α阳性区域AOD均呈先增加后减少趋势,而新生期和五月龄牦牛空肠和十二指肠IL-4阳性区域AOD无显著差异,成年期牦牛空肠IL-4阳性区域AOD显著增加,成年期牦牛十二指肠IL-4阳性区域AOD显著减少。本研究结果揭示了牦牛生长过程中VCAM-1、HIF-α和IL-4的表达与分布规律,为进一步探明牦牛肠道中低氧相关因子对环境的适应性提供了依据。
Abstract:
To clarify the differences in factors such as vascular cell adhesion molecule-1 (VCAM-1), hypoxia-inducible factor-1α (HIF-1α) and interleukin-4 (IL-4) in the intestinal tract of yaks of different age groups, the intestinal tissues ( ileum, jejunum and duodenum ) of healthy yaks in the three age groups of newborn, five-month-old and adult were selected in this study. Hematoxylin-Eosin (H&E) staining, Masson trichrome stain-ing, periodic acid-Schiff (PAS) staining and immunohistochemistry were used to explore the expression and distribution differences and age-related changes of vascular cell adhesion molecule-1 (VCAM-1), hypoxia-inducible factor-1α (HIF-1α) and interleukin-4 (IL-4) in the small intestine of yak. The results showed that, for newborn yak, the ratio of villus height to crypt depth (V/C) in duodenum was significantly higher than those in jejunum and ileum. Collagen fibers were mainly distributed in the duodenal gland area and lamina propria. The proportion of collagen fibers in the duodenum of newborn yaks was significantly higher than those of five-month-old and adult yaks, and the proportion of collagen fibers in the duodenum of newborn yaks was significantly higher than those of ileum and jejunum. The average optical density (AOD) of PAS staining of Paneth cells showed strong positive expression, while the positive expression of mucous cells, goblet cells and striated border was relatively weak. VCAM-1 was mainly distributed in the absorptive cells, lymphocytes, and striated border of the villus mucosa of yak small intestine. HIF-1α was positively expressed in many parts of yak small intestine, and IL-4 was enriched in the basement membrane, muscle fibers and lymphocytes of yak small intestine. With the increase of age, VCAM-1 AOD, HIF-1α AOD and IL-4 AOD in ileum of yak showed a significant decreasing trend, while VCAM-1 AOD and HIF-1α AOD in jejunum and duodenum increased first and then decreased. There was no significant difference in IL-4 AOD between jejunum and duodenum of newborn and five-month-old yak, while IL-4 AOD in jejunum of adult yak increased significantly, and IL-4 AOD in duodenum of adult yak decreased significantly. The results of this study revealed the expression and distribution of VCAM-1, HIF-α and IL-4 during the growth of yak, and provided a basis for further exploring the adaptability of hypoxia-related factors in the intestinal tract of yak to the environment.

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备注/Memo

备注/Memo:
收稿日期:2025-11-10基金项目:中央高校基本科研业务费专项资金项目(31920250002、31920250051、31920250071)；国家自然科学基金项目(31860687)；甘肃省青年人才项目(2025QNGR63)；甘肃省教育科学与技术创新项目(2023B-055)；甘肃省自然科学基金项目(24JRRA155)；兰州青年科技人才创新项目(2023-QN-70)作者简介:胡嘉琦(2002-),女,重庆人,硕士研究生,主要从事临床兽医学研究。(E-mail)3288423450@qq.com通讯作者:陈伟基,(E-mail)80191041@qq.com；杨琨,(E-mail)186152592@xbmu.edu.cn
更新日期/Last Update: 2026-09-16