[1]叶子安,田原,马辰昊,等.江苏常州地区PRRSV、PCV2、PCV3的流行特征及混合感染分析[J].江苏农业学报,2026,42(08):1608-1623.[doi:doi:10.3969/j.issn.1000-4440.2026.08.010]
　YE Zian,TIAN Yuan,MA Chenhao,et al.Analysis of epidemiological characteristics and co-infection of PRRSV, PCV2 and PCV3 in Changzhou, Jiangsu province[J].,2026,42(08):1608-1623.[doi:doi:10.3969/j.issn.1000-4440.2026.08.010]
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江苏常州地区PRRSV、PCV2、PCV3的流行特征及混合感染分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年08期
页码:
1608-1623
栏目:
畜牧兽医·水产养殖
出版日期:
2026-08-31

文章信息/Info

Title:
Analysis of epidemiological characteristics and co-infection of PRRSV, PCV2 and PCV3 in Changzhou, Jiangsu province
作者:
叶子安1; 田原2; 3; 马辰昊4; 罗坚强4
(1.中国农业大学动物医学院,北京100193；2.常州市武进区畜牧兽医管理服务中心,江苏常州213100；3.常州市武进区动物疫病预防控制中心,江苏常州213100；4.常州市动物疫病预防控制中心,江苏常州213000)
Author(s):
YE Zian1; TIAN Yuan2; 3; MA Chenhao4; LUO Jianqiang4
(1.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;2.Wujin Animal Husbandry and Veterinary Management Service Center, Changzhou 213100, China;3.Wujin Animal Disease Control Center, Changzhou 213100, China；4.Changzhou Animal Disease Control Center, Changzhou 213000, China)
关键词:
猪繁殖与呼吸综合征病毒; 猪圆环病毒2型; 猪圆环病毒3型; 混合感染; 流行病学; 净化
Keywords:
porcine reproductive and respiratory syndrome virus; porcine circovirus type 2; porcine circovirus type 3; co-infection; epidemiology; eradication
分类号:
S858.28
DOI:
doi:10.3969/j.issn.1000-4440.2026.08.010
文献标志码:
A
摘要:
为评估江苏常州地区猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型(PCV2)和猪圆环病毒3型(PCV3)的流行特征,本研究于2025年采集常州地区237份猪血清及对应拭子样本,使用商品化试剂盒检测抗体和病原,并对病原阳性样本进行目标基因扩增、测序及遗传进化分析。结果显示,PRRSV抗体阳性率为29.11%,场阳性率为16.67%；病原阳性率为15.61%,场阳性率为25.00%；8条基因序列均属PRRSV-2谱系1,与NADC30同源性最高。PCV2抗体阳性率为73.84%,场阳性率为83.33%；病原阳性率为86.92%,场阳性率为100.00%；7条ORF2基因序列均属PCV2d亚型。PCV3抗体阳性率为24.05%,场阳性率为66.67%；病原阳性率为56.12%,场阳性率为91.67%,10条ORF2基因序列均属PCV3a亚型。混合感染情况分析发现,以PCV2与PCV3共感染为主,阳性率为53.16%,场阳性率为91.67%；PRRSV与PCV2共感染阳性率为12.24%,场阳性率为25.00%；PRRSV与PCV3共感染以及三重感染阳性率均为8.44%,场阳性率均为16.67%。本研究结果表明,常州地区PRRSV呈现点状低流行特征,且毒株类型单一,多数非免疫场达到稳定控制状态,具备区域性净化基础；PCV2与PCV3处于高流行状态,共感染情况普遍,且现有PCV2疫苗难以阻断野毒株传播,建议优化防控策略。本研究结果为区域内动物疫病防控策略由免疫预防向精准控制与净化消灭转型提供了科学依据。
Abstract:
To assess the epidemiological characteristics of porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), and porcine circovirus type 3 (PCV3) in Changzhou, Jiangsu province, this study collected 237 porcine serum samples and corresponding swab samples from the region in 2025. Commercial kits were used to detect antibodies and pathogens. For pathogen-positive samples, target genes were amplified, sequenced, and subjected to phylogenetic analysis. The results showed that the PRRSV antibody positive rate was 29.11%, with a farm-level positive rate of 16.67%; the pathogen positive rate was 15.61%, with a farm-level positive rate of 25.00%. All eight obtained gene sequences belonged to PRRSV-2 lineage 1, sharing the highest homology with NADC30. For PCV2, the antibody positive rate was 73.84%, with a farm-level positive rate of 83.33%; the pathogen positive rate was 86.92%, with a farm-level positive rate of 100.00%. All seven ORF2 gene sequences belonged to the PCV2d subtype. For PCV3, the antibody positive rate was 24.05%, with a farm-level positive rate of 66.67%; the pathogen positive rate was 56.12%, with a farm-level positive rate of 91.67%. All ten ORF2 gene sequences belonged to the PCV3a subtype. Analysis of mixed infections revealed that co-infection of PCV2 and PCV3 was predominant, with a positive rate of 53.16% and a farm-level positive rate of 91.67%; the positive rate for PRRSV and PCV2 co-infection was 12.24%, with a farm-level positive rate of 25.00%; the positive rates for PRRSV and PCV3 co-infection and triple infection were both 8.44%, with farm-level positive rates of 16.67% for both. This study indicates that PRRSV in Changzhou exhibits a sporadic, low-prevalence pattern with a single virus strain type. Most non-vaccinated farms have achieved stable control, establishing a foundation for regional eradication. PCV2 and PCV3 are highly prevalent, with widespread co-infections. Current PCV2 vaccines struggle to block the transmission of field virus strains, suggesting the need for optimized prevention and control strategies. This research provides a scientific basis for transitioning regional animal disease prevention and control strategies from immunoprophylaxis to precision prevention, control, and eradication.

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备注/Memo

备注/Memo:
收稿日期：2026-03-23基金项目：常州市农业科技创新和示范推广项目[KCSF(2024)02]作者简介：叶子安(1999-),男,广东茂名人,硕士研究生,研究方向为动物分子病毒学与免疫学。(E-mail)zian_vet@163.com通讯作者：田原,(E-mail)missarah@126.com
更新日期/Last Update: 2026-09-16