[1]沙洁,杨涛,王琼,等.变形菌门复合菌剂与厚壁菌门复合菌剂叶面喷施对当归生理代谢、产量及抗根腐病的影响[J].江苏农业学报,2026,42(08):1575-1583.[doi:doi:10.3969/j.issn.1000-4440.2026.08.007]
　SHA Jie,YANG Tao,WANG Qiong,et al.Effects of foliar spraying of Proteobacteria compound bacterial inoculant and Firmicutes compound bacterial inoculant on physiological metabolism, yield and root rot resistance of Angelica sinensis[J].,2026,42(08):1575-1583.[doi:doi:10.3969/j.issn.1000-4440.2026.08.007]
点击复制

变形菌门复合菌剂与厚壁菌门复合菌剂叶面喷施对当归生理代谢、产量及抗根腐病的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年08期
页码:
1575-1583
栏目:
耕作栽培·资源环境
出版日期:
2026-08-31

文章信息/Info

Title:
Effects of foliar spraying of Proteobacteria compound bacterial inoculant and Firmicutes compound bacterial inoculant on physiological metabolism, yield and root rot resistance of Angelica sinensis
作者:
沙洁1; 杨涛1; 王琼2; 师立伟3; 赵东红4; 唐湛文5; 赵疆1; 党淑钟1
(1.甘肃省科学院生物研究所/甘肃省微生物资源开发利用重点实验室,甘肃兰州730000;2.岷县园艺指导站,甘肃岷县748400;3.定西市经济作物技术推广站,甘肃定西743000;4.岷县劲康中药材有限公司,甘肃岷县748400;5.重庆市药物种植研究所/重庆市道地药材规范化生产工程技术研究中心,重庆408435)
Author(s):
SHA Jie1; YANG Tao1; WANG Qiong2; SHI Liwei3; ZHAO Donghong4; TANG Zhanwen5; ZHAO Jiang1; DANG Shuzhong1
(1.Institute of Biology, Gansu Academy of Sciences/Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province, Lanzhou 730000, China;2.Minxian Horticultural Guidance Station, Minxian 748400, China;3.Dingxi Cash Crop Technology Extension Station, Dingxi 743000, China;4.Minxian Jinkang Chinese Medicinal Herbs Co., Ltd., Minxian 748400, China;5.Chongqing Institute of Medicinal Plant Cultivation/Chongqing Engineering Research Center for Standardized Cultivation of Genuine Medicinal Materials, Chongqing 408435, China)
关键词:
当归; 复合菌剂; 内源激素; 光合氮素利用效率; 产量; 品质
Keywords:
Angelica sinensis; compound bacterial agent; endogenous hormone; photosynthetic nitrogen use efficiency; yield; quality
分类号:
Q939.96
DOI:
doi:10.3969/j.issn.1000-4440.2026.08.007
文献标志码:
A
摘要:
本研究设置变形菌门复合菌剂(BL)、厚壁菌门复合菌剂(HH)及马铃薯葡萄糖肉汤(PDB)培养基(CK)3个处理,通过叶面喷施后测定当归植株的光合特性、抗氧化酶活性、氮素代谢、激素水平、产量及药效成分等指标,系统评估两种复合菌剂的应用效果。研究结果表明,BL处理和HH处理当归净光合速率、光合氮素利用效率显著高于对照(P＜0.05)。在抗氧化系统方面,BL处理当归的2,2′-联氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)和1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、过氧化物酶活性及总酚含量均显著高于对照(P＜0.05)。BL处理当归丙二醛含量显著低于对照(P＜0.05)。BL处理当归中茉莉酸类物质、细胞分裂素类物质积累量较高。HH处理当归中生长素、水杨酸类物质积累量较高。BL处理和HH处理当归中阿魏酸、阿魏酸松柏酯和Z-藁本内酯含量均显著高于对照(P＜0.05)。BL处理和HH处理当归不定根数、芦头直径、主根长、单株干质量显著高于对照(P＜0.05)。 BL处理和HH处理当归根腐病发病率均显著低于对照(P＜0.05)。两种菌剂处理均能够促进当归植株的生长发育,有效增强当归的抗病性,实现促进生长提高质量的良好效果。
Abstract:
In this study, three treatments were set, including the Proteobacteria compound bacterial agent (BL), the Firmicutes compound bacterial agent (HH), and the potato dextrose broth (PDB) medium control (CK). After foliar spraying, the photosynthetic characteristics, antioxidant enzyme activities, nitrogen metabolism, hormone levels, yield and medicinal components of Angelica sinensis plants were determined to systematically evaluate the application effects of the two compound bacterial agents. The results showed that the net photosynthetic rate and photosynthetic nitrogen use efficiency of Angelica sinensis under BL and HH treatments were significantly higher than those of the control (P＜0.05). In terms of the antioxidant system, the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacities, peroxidase activity and total phenol content of Angelica sinensis under BL treatment were significantly higher than those of the control (P＜0.05). The malondialdehyde content of Angelica sinensis under BL treatment was significantly lower than that of the control (P＜0.05). The accumulation of jasmonates and cytokinins in Angelica sinensis was higher under BL treatment, while the accumulation of auxins and salicylic acids was higher under HH treatment. The contents of ferulic acid, coniferyl ferulate and Z-ligustilide in Angelica sinensis under BL and HH treatments were significantly higher than those of the control (P＜0.05). The number of adventitious roots, rhizome diameter, main root length and dry weight per plant of Angelica sinensis under BL and HH treatments were significantly higher than those of the control (P＜0.05). The root rot incidence of Angelica sinensis under BL and HH treatments was significantly lower than that of the control (P＜0.05). Both bacterial agent treatments could promote the growth and development of Angelica sinensis plants, effectively enhance the disease resistance of Angelica sinensis, and achieve favorable effects of growth promotion and quality improvement.

参考文献/References:

[1]国家药典委员会. 中华人民共和国药典2020版:一部[M]. 北京:中国医药科技出版社,2020.
[2]Zhang H Y, Bi W G, Yu Y, et al. Angelica sinensis (Oliv.) Diels in China:distribution, cultivation,utilization and variation[J]. Genetic Resources and Crop Evolution,2012,59(4):607-613.
[3]张瑞福,沈其荣. 微生物肥料新型功能作用机理与根际定殖增强策略[J]. 微生物学杂志,2024,1(44):1-11.
[4]王建武,相微微,陈花,等. 沙地柏根际促生耐寒短杆菌SDB5的分离和功能鉴定[J]. 西北农业学报,2022,31(9):1201-1210.
[5]张萌,王逾涵,刘振宇,等. 接种根际促生菌对白花前胡根部品质与根际土壤磷、钾形态的影响[J]. 中草药,2025,56(3):987-997.
[6]罗守杨,田春杰,张加凡,等. 根际微生物对药材道地性的影响及应用前景展望[J]. 时珍国医国药,2022,33(4):948-950.
[7]孟彤彤,朱宁,崔兴帅,等. 党参内生细菌荧光假单胞菌RHBA17生物学特性及其对宿主的促生作用[J]. 甘肃农业大学学报,2023,4(58):146-155.
[8]Tsuda K, Tsuji G, Higashiyama M, et al. Biological control of bacterial soft rot in Chinese cabbage by Lactobacillus plantarum strain BY under field conditions[J]. Biological Control,2016,100:63-69.
[9]陈飘雪,陈飞,袁洁,等. 内生菌复合接种对茅苍术生长和倍半萜积累的影响[J]. 生态学杂志,2020,39(9):2944-2952.
[10]王香,温瑞琦,杨甜,等. 不同微生物菌剂对西瓜生长及根结线虫防治的影响[J]. 西北农业学报,2025,34(9):1658-1664.
[11]赵玲玉,索升州,赵祺,等. 梭梭根际促生菌(PGPR)菌肥对番茄产量、品质和土壤特性的影响[J]. 甘肃农业大学学报,2022,57(3):42-51.
[12]张文丽,万雨欣,徐伟慧,等. 促生菌种组合提高玉米根际土壤有益微生物基因丰度及促生效应研究[J]. 植物营养与肥料学报,2024,30(2):394-405.
[13]Gyaneshwar P, Kumar G N, Parekh L J, et al. Role of soil microorganisms in improving P nutrition of plants[J]. Plant and Soil,2002,245(1):83-93.
[14]Klironomos J N. Variation in plant response to native and exotic arbuscular mycorrhizal fungi[J]. Ecology,2003,84(9):2292-2301.
[15]任泉静. 接种外源微生物菌群对植物生长的影响及作用机制[D]. 郑州:河南农业大学,2025.
[16]梅惠敏,阮亚男,张家欣,等. 木霉对盐渍逆境下枸杞根系氮素吸收和氮素利用效率的影响[J]. 应用生态学报,2022,33(9):2539-2546.
[17]Chiappero J, Cappellari L D R, Alderete L G S, et al. Plant growth promoting rhizobacteria improve the antioxidant status in Mentha piperita grown under drought stress leading to an enhancement of plant growth and total phenolic content[J]. Industrial Crops and Products,2019,139:111553-111561.
[18]Meena M, Swapnil P, Divyanshu K, et al. PGPR-mediated induction of systemic resistance and physiochemical alterations in plants against the pathogens:current perspectives[J]. Journal of Basic Microbiology,2020,60(10):828-861.
[19]山雨思,代欢欢,何潇,等. 外源茉莉酸甲酯和水杨酸对盐胁迫下颠茄生理特性和次生代谢的影响[J]. 植物生理学报,2019,55(9):1335-1346.
[20]Yuan H M, Liu W C, Lu Y T. CATALASE2 coordinates SA-mediated repression of both auxin accumulation and JA biosynthesis in plant defenses[J]. Cell Host Microbe,2017,21(2):143-155.
[21]官玲亮,白琳,庞玉新,等. 外源水杨酸对艾纳香活性成分、抗氧化酶活力及内源激素含量的影响[J]. 中药材,2019,42(3):490-494.
[22]Wei X, Cao P, Wang G, et al. Microbial inoculant and garbage enzyme reduced cadmium (Cd) uptake in Salvia miltiorrhiza Bge. under Cd stress[J]. Ecotoxicology and Environmental Safety,2020,192:110311.
[23]Arzanesh H M, Alikhani A H, Khavazi K, et al. Wheat (Triticum aestivum L.) growth enhancement by Azospirillum sp. under drought stress[J]. World Journal of Microbiology and Biotechnology,2011,27(2):197-205.
[24]Kong X P, Zhang C L, Zheng H H. Antagonistic interaction between auxin and SA signaling pathways regulates bacterial infection through lateral root in Arabidopsis[J]. Cell Reports,2020,32(8):108060.

备注/Memo

备注/Memo:
收稿日期：2026-01-26基金项目：甘肃省科学院重点研发项目(2025QYXQ-02);甘肃省自然科学基金项目(23JRRA1657);甘肃省科技特派员(团)专项(25CXNJ007);平凉市科技计划项目(PL-STK-2024A-025)作者简介：沙洁(1984-),女,甘肃兰州人,硕士,助理研究员,主要从事微生物-植物互作研究。(E-mail)shajie_sj@163.com通讯作者：杨涛,(E-mail)yangtao8383@163.com
更新日期/Last Update: 2026-09-16