[1]陈丽,邰如倩,史宝,等.水稻果糖1,6-二磷酸酶调控对水稻条纹病毒和水稻黑条矮缩病毒的抗性[J].江苏农业学报,2026,42(08):1554-1563.[doi:doi:10.3969/j.issn.1000-4440.2026.08.005]
　CHEN Li,TAI Ruqian,SHI Bao,et al.Resistance to rice stripe virus and rice black-streaked dwarf virus regulated by rice fructose-1,6-biphosphatase[J].,2026,42(08):1554-1563.[doi:doi:10.3969/j.issn.1000-4440.2026.08.005]
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水稻果糖1,6-二磷酸酶调控对水稻条纹病毒和水稻黑条矮缩病毒的抗性()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年08期
页码:
1554-1563
栏目:
植物保护
出版日期:
2026-08-31

文章信息/Info

Title:
Resistance to rice stripe virus and rice black-streaked dwarf virus regulated by rice fructose-1,6-biphosphatase
作者:
陈丽1; 2; 邰如倩1; 2; 史宝1; 3; 李硕1; 邹保红2; 孙枫1; 季英华1; 3
(1.江苏省农业科学院植物保护研究所,江苏南京210014;2.南京农业大学农学院,江苏南京210095;3.青海大学农林科学院,青海西宁810016)
Author(s):
CHEN Li1; 2; TAI Ruqian1; 2; SHI Bao1; 3; LI Shuo1; ZOU Baohong2; SUN Feng1; JI Yinghua1; 3
(1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;3.Academy of Agriculture and Forestry Sciences, Qinhai University, Xining 810016, China)
关键词:
果糖1; 6-二磷酸酶; 水稻条纹病毒; 水稻黑条矮缩病毒; 病毒抗性
Keywords:
fructose-1; 6-biphosphatase; rice stripe virus; rice black-streaked dwarf virus; virus resistance
分类号:
Q945.8
DOI:
doi:10.3969/j.issn.1000-4440.2026.08.005
文献标志码:
A
摘要:
水稻条纹叶枯病和水稻黑条矮缩病分别由水稻条纹病毒(Rice stripe virus,RSV)和水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV)引起,是水稻生产过程中的重要病毒性病害,严重威胁着水稻的高产、稳产。利用植物抗性相关基因培育抗性水稻品种是防治水稻病毒病的有效措施,然而关于水稻抗病毒的机制还有待进一步解析。植物果糖1,6-二磷酸酶(FBPase)在糖异生代谢路径和蔗糖生物合成过程中起着关键作用。实时荧光定量逆转录聚合酶链式反应（qRT-PCR）检测结果表明,受到RBSDV、RSV侵染后,水稻植株中果糖1,6-二磷酸酶(OscFBP3,基因登录号:LOC_Os03g16050)的表达受到抑制。烟草叶片的瞬时表达试验结果表明,OscFBP3-YFP融合蛋白定位于细胞质,OscFBP3编码细胞质FBPase。本研究利用成簇规律间隔短回文重复序列及其相关蛋白9（CRISPR-cas9）技术构建了水稻oscfbp3突变体植株,分子鉴定结果表明,分别在oscfbp3 #5、oscfbp3 #6的OscFBP3靶点插入1个“A”或“T”,导致蛋白质翻译移码并提前终止。对水稻oscfbp3突变体接种RSV、RBSDV后,病毒的抗性鉴定结果表明,突变体植株对病毒的抗性减弱,并且其发病率显著高于野生型水稻植株。RNA和蛋白质分子检测结果进一步表明,突变体植株中的病毒积累量显著高于野生型水稻植株。田间农艺性状调查结果表明,与野生型水稻植株相比,水稻oscfbp3突变体植株表现出株高、单株分蘖数显著减少的生长发育表型。以上结果表明,水稻OscFBP3是调控水稻植株生长发育、病毒抗性的关键基因。本研究结果可为进一步了解水稻的抗病毒机制提供试验数据,并可为利用病毒抗性相关基因提高水稻品种的抗病性提供潜在靶标。
Abstract:
Rice stripe disease, caused by rice stripe virus (RSV), and rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (RBSDV), are major viral diseases in rice production, posing a serious threat to high and stable yield. Breeding resistant rice varieties using plant resistance-related genes is an effective strategy for controlling rice viral diseases; however, the mechanism underlying rice antiviral resistance remains to be further elucidated. Plant fructose-1,6-bisphosphatase (FBPase) plays a crucial role in the gluconeogenesis pathway and sucrose biosynthesis. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that the expression of OscFBP3 (gene accession number: LOC_Os03g16050) was suppressed in rice plants after RBSDV and RSV infection. Transient expression assay results in tobacco leaves revealed that the OscFBP3-YFP fusion protein was localized to the cytoplasm, confirming that OscFBP3 encoded a cytoplasmic FBPase. In this study, oscfbp3 mutant plants were generated using clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 （CRISPR-Cas9） technology. Molecular identification results showed that oscfbp3 #5 and oscfbp3 #6 carried a single-nucleotide insertion of “A” or “T” at the OscFBP3 target site (A in #5, T in #6), resulting in a translational frameshift and premature termination of protein translation. After inoculation of oscfbp3 mutants with RSV and RBSDV, virus resistance identification results showed that the mutant plants exhibited decreased resistance to the viruses, with a significantly higher disease incidence than wild-type plants. RNA and protein molecular detection results further demonstrated that virus accumulation levels were significantly higher in oscfbp3 mutant plants than in wild-type plants. Field agronomic trait survey results showed that, compared with wild-type plants, the plant height and tiller number per plant of oscfbp3 mutant plants were significantly reduced. These findings indicate that OscFBP3 is a key gene regulating plant growth, development, and virus resistance. The findings of this study provide experimental data for further understanding antiviral mechanisms in rice and offer potential targets for enhancing rice variety resistance through the use of virus resistance-related genes.

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备注/Memo

备注/Memo:
收稿日期：2026-02-02基金项目：国家重点研发计划项目(2023YFD1400300);江苏省自然科学基金项目(BK20231386)作者简介：陈丽(1996-),女,江西抚州人,硕士研究生,主要从事作物病毒防控技术研究。(E-mail)1509476226@qq.com通讯作者：孙枫,(E-mail)sunfeng1201@126.com;季英华,(E-mail)jiyinghua@jaas.ac.cn
更新日期/Last Update: 2026-09-16