[1]张茹艳,徐银萍,王炜,等.低氮条件下外源褪黑素调控青稞幼苗生长和氮素吸收利用的生理与分子机制[J].江苏农业学报,2026,42(08):1534-1541.[doi:doi:10.3969/j.issn.1000-4440.2026.08.003]
　ZHANG Ruyan,XU Yinping,WANG Wei,et al.Physiological and molecular mechanisms of exogenous melatonin in regulating growth and nitrogen uptake and utilization of highland barley seedlings under low nitrogen conditions[J].,2026,42(08):1534-1541.[doi:doi:10.3969/j.issn.1000-4440.2026.08.003]
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低氮条件下外源褪黑素调控青稞幼苗生长和氮素吸收利用的生理与分子机制()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年08期
页码:
1534-1541
栏目:
遗传育种·生理生化
出版日期:
2026-08-31

文章信息/Info

Title:
Physiological and molecular mechanisms of exogenous melatonin in regulating growth and nitrogen uptake and utilization of highland barley seedlings under low nitrogen conditions
作者:
张茹艳1; 徐银萍2; 王炜1; 郭小花1; 陈军1; 孙夏玲1; 陈子萱1
(1.甘肃省农业科学院生物技术研究所,甘肃兰州730070；2.甘肃省农业科学院经济作物与啤酒原料研究所,甘肃兰州730070)
Author(s):
ZHANG Ruyan1; XU Yinping2; WANG Wei1; GUO Xiaohua1; CHEN Jun1; SUN Xialing1; CHEN Zixuan1
(1.Institute of Biotechnology, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China；2.Institute of Cash Crops and Malting Raw Materials, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China)
关键词:
青稞; 褪黑素; 低氮胁迫; 氮素吸收
Keywords:
highland barley; melatonin; low-nitrogen stress; nitrogen uptake
分类号:
S512.3
DOI:
doi:10.3969/j.issn.1000-4440.2026.08.003
文献标志码:
A
摘要:
本研究以青稞品种陇青1号为试验材料,探究外源施用褪黑素对不同氮素水平下青稞幼苗生长、氮素吸收及相关基因表达的影响。结果表明,褪黑素处理能够显著促进低氮条件下青稞幼苗地上部与地下部的生长,并改善根系形态(包括根长、根体积、根表面积、根直径及根尖数),同时提高青稞幼苗地上部与地下部的全氮含量与氮素吸收量。在低氮条件下,褪黑素处理青稞幼苗地上部谷氨酰胺合成酶基因(HvGS2)、硝酸盐转运蛋白基因(HvNRT3.1和HvNRT3.3)相对表达量显著高于对照(P＜0.05),褪黑素处理青稞幼苗地下部硝酸盐转运蛋白基因(HvNRT3.1)相对表达量显著高于对照(P＜0.05)，表明褪黑素能够系统性地增强青稞地上部和地下部的氮还原与同化能力,从而协同提升植株对氮素的吸收与利用效率。综上,外源褪黑素可通过优化根系构型、增强氮素吸收与氮代谢基因的表达,有效缓解低氮胁迫,促进青稞幼苗生长。本研究结果为低氮环境下青稞的高产栽培提供了理论依据。
Abstract:
In this study, the highland barley cultivar Longqing No.1 was used as experimental material to investigate the effects of exogenous melatonin application on the growth, nitrogen uptake and expression of related genes in highland barley seedlings under different nitrogen levels. The results showed that melatonin treatment significantly promoted the growth of both aboveground and underground parts of highland barley seedlings under low nitrogen conditions, and improved root morphology, including root length, root volume, root surface area, root diameter and root tip number. Meanwhile, the total nitrogen content and nitrogen uptake in the aboveground and underground parts of seedlings were increased. Under low nitrogen conditions, the relative expression levels of glutamine synthetase gene (HvGS2) and nitrate transporter genes (HvNRT3.1 and HvNRT3.3) in the aboveground parts of melatonin-treated seedlings were significantly higher than those in the control group (P＜0.05). Similarly, the relative expression levels of HvNRT3.1 in underground parts were also markedly upregulated compared with the control (P＜0.05). These findings indicate that melatonin can systematically enhance nitrogen reduction and assimilation capacity in both aboveground and underground tissues, thereby jointly improving nitrogen uptake and utilization efficiency of plants. In conclusion, exogenous melatonin can effectively alleviate low-nitrogen stress and promote the growth of highland barley seedlings by optimizing root architecture and upregulating the expression of genes involved in nitrogen uptake and metabolism. This study provides a theoretical basis for high-yield cultivation of highland barley under low nitrogen conditions.

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备注/Memo

备注/Memo:
收稿日期：2025-12-08基金项目：国家自然科学基金项目(32260499)；甘肃省农业科学院现代生物育种项目(2023GAAS13)作者简介：张茹艳(1995-),女,甘肃华池人,博士,助理研究员,主要从事作物细胞工程与分子育种研究。(E-mail)zhangruyan1031@163.com。徐银萍为共同第一作者。通讯作者：王炜,(E-mail)sjswangwei@gsagr.cn
更新日期/Last Update: 2026-09-16