[1]谢少和,陈锦文,陈惠清,等.基于绿色基因指纹的优质稻品种古早占的选育及应用评价[J].江苏农业学报,2026,42(08):1513-1522.[doi:doi:10.3969/j.issn.1000-4440.2026.08.001]
　XIE Shaohe,CHEN Jinwen,CHEN Huiqing,et al.Breeding and application evaluation of the high-quality rice variety Guzaozhan based on green gene fingerprinting[J].,2026,42(08):1513-1522.[doi:doi:10.3969/j.issn.1000-4440.2026.08.001]
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基于绿色基因指纹的优质稻品种古早占的选育及应用评价()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年08期
页码:
1513-1522
栏目:
遗传育种·生理生化
出版日期:
2026-08-31

文章信息/Info

Title:
Breeding and application evaluation of the high-quality rice variety Guzaozhan based on green gene fingerprinting
作者:
谢少和1; 陈锦文1; 陈惠清1; 余文昌1; 侯凯强1; 谢旺有1; 王天生1; 曾红英1; 田大刚2
(1.泉州市农业科学研究所,福建晋江362212；2.福建省农业科学院生物技术研究所,福建福州350003)
Author(s):
XIE Shaohe1; CHEN Jinwen1; CHEN Huiqing1; YU Wenchang1; HOU Kaiqiang1; XIE Wangyou1; WANG Tiansheng1; ZENG Hongying1; TIAN Dagang2
(1.Quanzhou Institute of Agricultural Sciences, Jinjiang 362212, China；2.Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China)
关键词:
优质稻; 绿色性状有利等位基因; 40 K绿色基因芯片; 配合力
Keywords:
high-quality rice; favorable alleles for green traits; 40 K green gene chip; combining ability
分类号:
S511
DOI:
doi:10.3969/j.issn.1000-4440.2026.08.001
文献标志码:
A
摘要:
为选育资源节约、环境友好型绿色超级稻品种,本研究利用包含115个功能位点的水稻40 K绿色基因芯片,对53份高代株系开展基因型鉴定,筛选得到聚合大量绿色功能基因的籼稻品种古早占。以古早占、华占、佳禾165为父本,与7份不育系开展不完全双列杂交,配制21个杂交组合,系统测定各组合农艺性状并解析配合力效应。结果表明,古早占携带48个绿色性状有利等位基因,其中47个可追溯至3个亲本；相关功能基因呈功能关联区段、模块式成簇分布,赋予该种质多重逆境抗性潜力。配合力分析结果显示,古早占产量性状一般配合力表现中等(一般配合力为-0.61),但与元亨S配组时特殊配合力高达19.56,该组合单株产量达152.53 g,穗长、千粒质量优势突出。方差分析结果证实，不育系、恢复系基因型及其互作均显著或极显著调控杂交后代产量。综上,本研究选育了聚合48个绿色功能基因的优异种质古早占,该种质与特定遗传背景不育系配组可充分发挥高产杂种优势,为南方绿色超级稻分子设计育种提供了核心种质与理论支撑。
Abstract:
To breed resource-saving and environment-friendly green super rice varieties, this study employed a rice 40 K green gene chip, covering 115 functional loci, for precise genotyping of 53 advanced lines. A line aggregating the highest number of green functional genes was selected and named Guzaozhan (GZZ). An incomplete diallel cross was carried out between seven sterile lines and three male parents (GZZ, Huazhan and Jiahe 165), yielding 21 hybrid combinations. Agronomic traits and combining ability were systematically evaluated. Haplotype analysis revealed that the conventional indica variety GZZ carried 48 favorable alleles for green traits, 47 of which could be traced back to three parents. These genes were distributed in functionally related clusters or modular patterns within the genome, indicating potential for multiple stress resis-tance. Combining ability analysis showed that GZZ exhibited moderate general combining ability (GCA=-0.61) for yield traits, but its specific combining ability with Yuanheng S reached 19.56. This combination achieved a grain yield per plant of 152.53 g and showed significant advantages in panicle length and 1 000-grain weight. Analysis of variance confirmed that the genotypes of sterile lines and restorer lines, as well as their interaction, had highly significant or significant effects on hybrid progeny yield. This study successfully identified and developed a new elite germplasm, GZZ, which aggregates 48 green functional genes. When crossed with sterile lines carrying specific genetic backgrounds, this germplasm can fully exert high-yield heterosis, providing core germplasm and theoretical support for molecular design breeding of green super rice in southern China.

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备注/Memo

备注/Memo:
收稿日期：2026-02-05基金项目：福建省自然科学基金重点项目(2022J02010)；福建省科技计划引导性项目(2023N0052、2025N0037)；福建省科技计划星火项目(2024S0029、2025S0030)；2025年福建省特色现代农业发展专项(设区市农科院所建设)[闽财农指(2025)86号]；2024-2025年福建省现代水稻产业技术体系建设项目[闽农科教(2024)20号]；“十四五”福建省种业创新与产业化工程项目(zycxny2021001)作者简介：谢少和(1973-),男,福建屏南人,学士,副研究员,从事水稻遗传育种与栽培技术研究。(E-mail)360328296@qq.com通讯作者：田大刚,(E-mail)tdg@fjage.org
更新日期/Last Update: 2026-09-16