[1]姜聖姬,王淑安,张恩亮,等.紫薇遗传图谱构建及株型性状的QTL定位[J].江苏农业学报,2023,(09):1818-1826.[doi:doi:10.3969/j.issn.1000-4440.2023.09.003]
　JIANG Sheng-ji,WANG Shu-an,ZHANG En-liang,et al.Construction of genetic linkage map and QTL mapping of plant architecture in Lagerstroemia indica[J].,2023,(09):1818-1826.[doi:doi:10.3969/j.issn.1000-4440.2023.09.003]
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紫薇遗传图谱构建及株型性状的QTL定位()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年09期
页码:
1818-1826
栏目:
遗传育种·生理生化
出版日期:
2023-12-31

文章信息/Info

Title:
Construction of genetic linkage map and QTL mapping of plant architecture in Lagerstroemia indica
作者:
姜聖姬; 王淑安; 张恩亮; 李林芳; 高露璐; 杨如同; 王鹏
(江苏省中国科学院植物研究所,江苏南京210014)
Author(s):
JIANG Sheng-ji; WANG Shu-an; ZHANG En-liang; LI Lin-fang; GAO Lu-lu; YANG Ru-tong; WANG Peng
(Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China)
关键词:
紫薇; 简单重复序列(SSR)标记; 遗传图谱; 株型; 数量性状座(QTL)定位
Keywords:
Lagerstroemia indica; simple sequence repeat (SSR) markers; genetic linkage map; plant architecture; quantitative trait locus (QTL) mapping
分类号:
S685.99
DOI:
doi:10.3969/j.issn.1000-4440.2023.09.003
文献标志码:
A
摘要:
紫薇是中国重要的木本观花植物,株型是其重要的观赏性状,但是目前尚未见关于其株型数量性状基因座(QTL)定位的研究。以紫薇金幌、堇秀构建的181个F1代分离群体为研究材料,构建1张由36个连锁群组成的包含429个SSR标记位点的遗传连锁图谱,总遗传距离为1 998.81 cM,位点间平均距离为4.63 cM；LG1连锁群的遗传距离最大,为177.60 cM,LG27连锁群覆盖的遗传距离最小,为19.32 cM。基于区间作图法共检测到7个株型的QTL位点,其中控制株高的QTL位点有4个,解释4.32%～56.93%的表型变异；控制地径的QTL位点有2个,分别解释56.43%和59.02%的表型变异；控制分枝数的QTL位点有1个,解释54.60%的表型变异。本研究率先构建了基于功能分子标记的紫薇种内遗传连锁图谱,并定位了7个紫薇株型的QTL位点,可以解释4.32%～59.02%的表型变异。研究结果可为紫薇基因定位、基因克隆及分子标记辅助选择奠定基础。
Abstract:
Lagerstroemia indica is an important woody ornamental plant in China, and plant type is an important ornamental trait. However, the research on quantitative trait locus (QTL) mapping of plant type has not been reported. In this study, 181 F1 segregation populations of Jinhuang and Jinxiu were used as research materials, and a genetic linkage map consisting of 36 linkage groups and 429 SSR marker loci was constructed. The total genetic distance was 1 998.81 cM, and the average distance between loci was 4.63 cM. The genetic distance of LG1 linkage group was the largest, which was 177.60 cM, and the total genetic distance covered by LG27 linkage group was the smallest, which was 19.32 cM. Based on the interval mapping method, a total of seven QTLs for plant type were detected, including four QTLs for plant height, explaining 4.32%-56.93% of the phenotypic variation. There were two QTLs controlling ground diameter, explaining 56.43% and 59.02% of phenotypic variation. There was one QTL locus controlling the number of branches, explaining 54.60% of the phenotypic variation. In this study, an intraspecific genetic linkage map of L.indica based on functional molecular markers was first constructed, and seven QTLs of L.indica plant type were mapped, explaining 4.32%-59.02% of phenotypic variation. In conclusion, these results can lay a foundation for gene mapping, gene cloning and molecular marker-assisted selection of Lagerstroemia indica.

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备注/Memo

备注/Memo:
收稿日期:2022-12-02基金项目:中央财政林业科技推广示范资金项目[苏(2021)TG07]；国家自然科学基金面上项目(31770745)作者简介:姜聖姬(1996-),女,辽宁东港人,硕士研究生,主要从事植物遗传学研究。(E-mail)15295776171@163.com。王淑安为共同第一作者。通讯作者:王鹏,(E-mail)wp280018@163.com
更新日期/Last Update: 2024-01-15