[1]宋志姣,李志强,李建珊,等.红泡刺藤不同部位总黄酮提取及其抗氧化性[J].江苏农业学报,2020,(03):727-732.[doi:doi:10.3969/j.issn.1000-4440.2020.03.027]
 SONG Zhi-jiao,LI Zhi-qiang,LI Jian-shan,et al.Extraction of total flavonoids from different parts of Rubus niveus and evaluation of antioxidant activity[J].,2020,(03):727-732.[doi:doi:10.3969/j.issn.1000-4440.2020.03.027]
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红泡刺藤不同部位总黄酮提取及其抗氧化性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年03期
页码:
727-732
栏目:
加工贮藏·质量安全
出版日期:
2020-06-30

文章信息/Info

Title:
Extraction of total flavonoids from different parts of Rubus niveus and evaluation of antioxidant activity
作者:
宋志姣李志强李建珊李悦杨丽华章金龙李晓娇
(保山学院资源环境学院,云南保山678000)
Author(s):
SONG Zhi-jiaoLI Zhi-qiangLI Jian-shanLI YueYANG Li-huaZHANG Jin-longLI Xiao-jiao
(College of Resources and Environmental Sciences, Baoshan University, Baoshan 678000, China)
关键词:
红泡刺藤总黄酮提取工艺抗氧化活性
Keywords:
Rubus niveus Thunb.total flavonoidsextraction processantioxidant activity
分类号:
S567.9
DOI:
doi:10.3969/j.issn.1000-4440.2020.03.027
文献标志码:
A
摘要:
以红泡刺藤叶片为材料,优化超声波辅助乙醇提取总黄酮的提取工艺;在最优提取条件下探讨红泡刺藤不同器官总黄酮含量和抗氧化性差异。通过正交试验,确定了红泡刺藤叶总黄酮最佳提取工艺:乙醇体积分数40%,料液比1∶30 (g/ml),超声波提取功率175 W,超声波提取时间45 min。在此条件下红泡刺藤根、茎、叶和果实总黄酮含量分别为24.66 mg/g、19.06 mg/g、28.07 mg/g和4.05 mg/g。红泡刺藤根、茎、叶和果实的总黄酮具有较强的抗氧化活性,对羟基自由基有很好的清除效果,其IC50值分别为1.620 mg/L、0.537 mg/L、3.655 mg/L和1.499 mg/L,清除能力排序为茎总黄酮>果实总黄酮>根总黄酮>叶总黄酮;其DPPH自由基的IC50值分别为7.856 mg/L、3.559 mg/L、5.481 mg/L和5.574 mg/L,清除能力大小为茎总黄酮>叶总黄酮>果实总黄酮>根总黄酮,抗氧化性均显著高于VC。
Abstract:
In this study, the leaves of Rubus niveus were used as test materials, and the extraction process of total flavonoids by ultrasound-assisted extraction(UAE) with ethanol was optimized. Herein, the comparison of the total flavonoids contents and antioxidant activities in different organs of R. niveus was carried out under the optimal conditions. Four factors (ethanol concentration, ratio of material to ethanol, ultrasonic power and extraction time) were evaluated to determine the optimal extraction conditions by using orthogonal design. The optimal extraction conditions were as follows: concentration of ethanol was 40%, material to ethanol ratio was 1∶30 (g/ml), ultrasonic power was 175 W, and extraction time was 45 min. Under the optimal extraction conditions, the total flavonoids contents in roots, stems, leaves and fruits of R. niveus were 24.66 mg/g, 19.06 mg/g, 28.07 mg/g and 4.05 mg/g, respectively. The total flavonoids extracted from roots, stems, leaves and fruits showed strong antioxidant activity and high hydroxyl radical scavenging activity, and IC50 values were 1.620 mg/L, 0.537 mg/L, 3.655 mg/L and 1.499 mg/L, respectively. Thus, the hydroxyl radical scavenging abilities of extracts followed the order of stems>fruits>roots>leaves. In the DPPH free radical scavenging assay, the IC50 values of these organs were 7.856 mg/L, 3.559 mg/L, 5.481 mg/L and 5.574 mg/L. The DPPH free radical scavenging abilities of extracts followed the order of stems>leaves>fruits>roots. The results also showed that the antioxidant activities of total flavonoids in these different organs were significantly higher than VC.

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备注/Memo

备注/Memo:
收稿日期:2019-10-11基金项目:云南省科技厅项目(2018FD097);2017年中央财政支持地方高校发展专项资金保山学院资源环境学院高原特色农业创新团队建设项目;保山市“第八批中青年学术和技术带头人”项目(201903);保山市第八批创新团队项目(201911)作者简介:宋志姣(1988-),女,云南保山人,博士,副教授,主要从事植物资源利用研究。(E-mail)janeolo@163.com通讯作者:李晓娇,(E-mail)lixiaojiao0622@163.com
更新日期/Last Update: 2020-07-14