[1]王旭堂,刁志祥,张培杨,等.超高液相色谱荧光检测法测定鹌鹑蛋和鸽蛋中甲砜霉素残留量[J].江苏农业学报,2020,(01):206-211.[doi:doi:10.3969/j.issn.1000-4440.2020.01.029]
　WANG Xu-tang,DIAO Zhi-xiang,ZHANG Pei-yang,et al.Determination of thiamphenicol residues in quail eggs and pigeon eggs by ultra-high performance liquid chromatography with fluorescence detection[J].,2020,(01):206-211.[doi:doi:10.3969/j.issn.1000-4440.2020.01.029]
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超高液相色谱荧光检测法测定鹌鹑蛋和鸽蛋中甲砜霉素残留量()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年01期
页码:
206-211
栏目:
加工贮藏·质量安全
出版日期:
2020-02-29

文章信息/Info

Title:
Determination of thiamphenicol residues in quail eggs and pigeon eggs by ultra-high performance liquid chromatography with fluorescence detection
作者:
王旭堂1; 2; 刁志祥1; 2; 张培杨1; 2; 谢恺舟1; 2; 王波2; 3; 刘楚君1; 2; 张跟喜1; 2; 张涛1; 2; 刘学忠2; 3; 戴国俊1; 2
（1.扬州大学动物科学与技术学院，江苏扬州225009；2.教育部农业与农产品安全国际合作联合实验室，江苏扬州225009；3.扬州大学兽医学院，江苏扬州225009）
Author(s):
WANG Xu-tang1; 2; DIAO Zhi-xiang1; 2; ZHANG Pei-yang1; 2; XIE Kai-zhou1; 2; WANG Bo2; 3; LIU Chu-jun1; 2; ZHANG Gen-xi1; 2; ZHANG Tao1; 2; LIU Xue-zhong2; 3; DAI Guo-jun
(1.College of Animal Science and Technology， Yangzhou University， Yangzhou 225009， China；2.Joint InternationalReearch Laboratory of Agriculture & Agri-Product Safety， Ministry of Education， Yangzhou 225009， China；3.College of Veterinary Medicine， Yangzhou University， Yangzhou 225009， China)
关键词:
鹌鹑蛋; 鸽蛋; 甲砜霉素; 加速溶剂萃取; 超高效液相色谱-荧光检测法
Keywords:
quail eggs; pigeon eggs; thiamphenicol; accelerated solvent extraction; ultra-high performance liquid chromatography with fluorescence detection
分类号:
S859.84
DOI:
doi:10.3969/j.issn.1000-4440.2020.01.029
文献标志码:
A
摘要:
本研究旨在建立鹌鹑蛋和鸽蛋中甲砜霉素（TAP）残留的超高效液相色谱-荧光检测（UPLC-FLD）方法。以鹌鹑蛋和鸽蛋为试验材料，采用加速溶剂萃取（ASE）技术提取目标物，以乙腈饱和的正己烷去脂，净化后用UPLC-FLD法进行检测分析。结果表明，在鹌鹑蛋和鸽蛋中TAP的添加质量比在定量限（LOQ）至250.0 μg/kg内，线性关系良好，决定系数均大于0.999 2。TAP添加质量比为LOQ、0.5 MRL、1.0 MRL和2.0 MRL时，在鹌鹑蛋和鸽蛋中的回收率分别为83.23%～95.72%、84.37%～97.12%，检测限（LOD）分别为3.4 μg/kg、3.3 μg/kg，LOQ分别为9.9 μg/kg、9.7 μg/kg。检测方法快速、高效、灵敏度高，为鹌鹑蛋和鸽蛋中TAP残留检测提供了新方法。
Abstract:
This study aimed to establish an ultra-high performance liquid chromatography-fluorescence detection (UPLC-FLD) method for the determination of thiamphenicol (TAP) residues in quail eggs and pigeon eggs. Using quail eggs and pigeon eggs as test materials, the target compound was extracted by accelerated solvent extraction (ASE) technique, and degreased by n-hexane saturated with acetonitrile. After purification, it was detected and analyzed by UPLC-FLD method. The results showed that the mass ratio of TAP in quail eggs and pigeon eggs was in the range of the limit of quantification (LOQ) to 250.0 μg/kg, and the linear relationship was good. The coefficients of determination were greater than 0.999 2. When TAP was added at mass ratios of LOQ, 0.5 maximum residue limit (MRL), 1.0 MRL and 2.0 MRL, the recoveries in quail eggs and pigeon eggs were 83.23%-95.72%, 84.37%-97.12%, the detection limits were 3.4 μg/kg and 3.3 μg/kg, and the quantitative limits were 9.9 μg/kgand 9.7 μg/kg, respectively. The detection method used in this study is rapid, efficient and sensitive, which provides a new method for the detection of TAP residues in quail eggs and pigeon eggs.

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备注/Memo

备注/Memo:
收稿日期：2019-06-26基金项目：国家现代农业产业技术体系项目（CARS-41-G23）；扬州大学“高端人才支持计划”项目(PAPD); 江苏省农业科技自主创新基金项目[CX（18）3022]作者简介：王旭堂（1994-），男，甘肃武威人，硕士研究生，主要从事畜产品安全与检测。( E-mail)2916502606@qq.com通讯作者：谢恺舟， ( E-mail)yzxkz168@163.com
更新日期/Last Update: 2020-03-13